LI Xiaoyue,YUAN Yanwen,GU Chenran,et al.Effects of Environmental Factors and Genotypes on Cucumber Fruit Hollow Trait[J].Northern Horticulture,2023,(16):18-25.[doi:10.11937/bfyy.20224884]
不同环境因子和基因型对黄瓜空腔性状的影响
- Title:
- Effects of Environmental Factors and Genotypes on Cucumber Fruit Hollow Trait
- 文献标志码:
- A
- 摘要:
- 以6个不同基因型的黄瓜品系作为试材，采用正交实验法，研究不同灌水量、施氮量和温度条件对黄瓜果实空腔性状的影响；同时，对6份不同基因型黄瓜果实空腔的生理生化指标和显微结构进行测定分析,以期为黄瓜果实空腔性状的研究及黄瓜无空腔品种的选育提供参考依据。结果表明：3种环境因子影响黄瓜果实空腔性状的主次顺序为施氮量>温度>灌水量，施氮量和温度与黄瓜果实空腔等级呈显著正相关；不同基因型黄瓜果皮叶绿素、可溶性蛋白质与空腔等级呈显著负相关，ABA含量呈显著正相关，可溶性糖、可溶性固形物含量与空腔等级无显著相关性。其 中，果皮叶绿素和可溶性蛋白质含量可作为黄瓜空腔的鉴定和预测指标；石蜡切片观察发现，果实空腔越大的黄瓜其果肉细胞可能越小。
- Abstract:
- In this study，six different genotypes of cucumber were used as experimental materials to investigate the effects of different irrigation levels，nitrogen application rates，and temperature conditions on cucumber fruit hollow characteristics using an orthogonal experimental design.Additionally，physiologyical and biochemical indexes and microscopic were conducted and analyzed on the fruit cavities of the six genotypes,in order to provide reference for the study of cucumber fruit hollow traits and the breeding of hollow-free cucumber varieties.The results showed that among the three environmental factors，nitrogen application rate had the greatest influence on cucumber fruit hollow characteristics，followed by temperature and irrigation level.Both nitrogen application rate and temperature showed a significant positive correlation with cucumber fruit hollow grade.Furthermore，there was a significant negative correlation between chlorophyll and soluble protein content of cucumber fruit peel and hollow grade，while ABA content exhibited a significant positive correlation.However，soluble sugar and soluble solid content showed no significant correlation with hollow grade.Among these factors，chlorophyll and soluble protein content in fruit peel could be used as identification and prediction indicators for cucumber fruit hollow.Observation of paraffin sections indicated that cucumbers with larger fruit cavities may have smaller flesh cells.
参考文献/References:
[1]商庆梅，秦智伟，周秀艳.黄瓜植株衰老过程 中根系内生理生化指标变化[J].东北农业大学学报，2010，41(9)：27-30.[2]李博，秦智伟，周秀艳.黄瓜果肉颜色遗传分析及SSR分子标记[J].东北农业大学学报，2010，41(12)：21-25.[3]王敏，刘书林，张圣平，等.黄瓜RIL群体瓜长和把长的qtl定位分析[J].西北植物学报，2014，34(9)：1764-1770.[4]JIANG L，YAN S S，YANG W C，et al.Transcriptomic analysis reveals the roles of microtubulE-related genes and transcription factors in fruit length regulation in cucumber(Cucumis sativus L.)[J].Scientific Reports，2015(5):12-19.[5]巩磊，夏杜菲，王文娇，等.光钙耦合对黄瓜植株生长、叶片叶绿素荧光特性和果实品质的影响[J].北方园艺，2022(5)：1-7.[6]LIU H Q，MENG H W，PAN Y P，et al.Fine genetic mapping of the white immature fruit color gene W to a 330-Kb region in cucumber (Cucumis sativus L.)[J].Theoretical And Applied Genetics，2015,128(12)：2375-2385.[7]苍涛，宋雯，赵学平，等.氯吡脲对草莓空心与畸形的影响[J].浙江农业学报，2017,29(9)：1537-1543.[8]千春录.黄瓜果实成熟度与耐冷性的关系及其生理机制研究[D].杭州：浙江大学，2012.[9]魏珑.果蔬叶绿素降解机理研究进展[J].食品安全导刊，2016(9)：49-50.[10]JIMENZ A，CREISSEN G，KULAR B，et al.Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening[J].Planta，2002,214(5)：751-758.[11]HUANG R，XIA R，HU L，et al.Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation[J].)Scientia Horticulturae，2007，113(2)：166-172.[12]ZHOU G，CHEN C，LIU X H，et al.The formation of hollow trait in cucumber (Cucumis sativus L.) fruit is controlled by CsALMT2[J].International Journal of Molecular Sciences，2022，23(11)：6173.[13]范晨伟，田丽波，商桑，等.黄瓜果实內源激素与果实空腔的关系[J].南方农业学报,2022，53(11)：3174-3183.[14]WANG Y H，JIANG B，DYMERSKI R，et al.Quantitative trait loci for horticulturally important traits defining the sikkim cucumber，Cucumis sativus var.Sikkimensis[J].Theoretical And Applied Genetics，2021，134(1)：229-247.[15])LIU B，LIU X W，YANG S，et al.Silencing of the gibberellin receptor Homolog, CsGID1a，affects locule formation in cucumber (Cucumis sativus) fruit[J].New Phytologist，2016，210(2)：551-563.[16]ZHOU G,CHEN C,LIU X H,et al.The formation of hollow trait in cucumber (Cucumis sativus L.) fruit is controlled by CsALMT2[J].International Journal of Molecular Sciences,2022,23(11):6173.[17]蒋静静，常晓晓，胡晓辉.供氮水平对基质袋培黄瓜养分吸收分配和产量的影响[J].浙江大学学报(农业与生命科学版)，2018，44(6)：678-686.[18]马鸿艳.温室不同温度管理对黄瓜生长、产量及理化指标的影响[D].哈尔滨：东北农业大学，2004.[19]连兆煌.无土栽培原理与技术[M].北京： 中国农业出版社，1992.[20]孙嫣然，秦智伟，周秀艳，等.不同因素对黄瓜丙醇二酸含量的影响[J]. 中国农学通报，2013，29(34)：85-89.[21]赵世杰，史国安，董新纯.植物生理学实验指导[M].北京： 中国农业科学技术出版社，2004.[22]曹建康，姜微波，赵玉梅.果蔬采后生理生化实验指导[M].北京： 中国轻工业出版社，2007.[23]李合生，陈翠莲，洪玉枝，等.植物生理生化实验原理和技术[M].北京：高等教育出版社，2000.[24]张政宪，谭桂茹，黄元极，等.植物生理学实验技术[M].沈阳：辽宁技术出版社，1989.[25]KAYMAK H C，GUVENC I，GUROL A.Correlation between endogenous elements and development of hollowing in the root of radish (Raphanus sativus L.) cultivars[J].ZemdirbystE-agriculture，2010，97(3)：97-104.[26]AKOUMIANAKIS K A，KARAPANOS I C，GIAKOUMAKI M，et al.Nitrogen，season and cultivar affect radish growth，yield，sponginess and hollowness[J].International Journal Of Plant Production，2011，5(2)：111-119.[27]林建华.西瓜各个时期出现的异常现象及克服措施[J]. 中国西瓜甜瓜，2005(2)：52-53.[28]BUSSAN A J.The Canon of potato science：Brown centre and hollow heart[J].Potato Research，2007，50(3/4)：395-398.[29]介岗牛，任小勇.白芦笋空心的发生与预防[J].山西农业，2004(2)：19-20.[30]YU H F，WANG J S，ZHAO Z Q，et al.Construction of a high-density genetic map and identification of loci related to hollow stem trait in broccoli(Brassic oleracea L.Italica)[J].Frontiers in Plant Science，2019(10):45.[31]陈小鹏.黄瓜果实发育与内源激素含量关系的研究[D].泰安：山东农业大学，2005.
相似文献/References:
[1]李亚芹,郑根昌.冬棚黄瓜的氮磷钾肥配施效应分析[J].北方园艺,2014,38(12):146.
LI Ya-qin,ZHENG Gen-chang. Effect Analysis of Application of N,P and K Fertilizers on Cucumber in Winter Plastic Greenhouses [J].Northern Horticulture,2014,38(16):146.
[2]由玉卿,秦智伟,周秀艳,等.黄瓜果实中丙醇二酸含量的配合力分析[J].北方园艺,2014,38(10):19.
YOU Yu-qing,QIN Zhi-wei,ZHOU Xiu-yan,et al.Analysis on Combining Ability of Tartronic Acid Content in Cucumber[J].Northern Horticulture,2014,38(16):19.
[3]金风霞,麻冬梅,武东波,等.不同种植年限苜蓿叶片光合生理生态特性的研究[J].北方园艺,2013,37(22):70.
JIN Feng-xia,MA Dong-mei,WU Dong-bo,et al.Study on Ecophysiological Characteristics of Leaf Photosynthesis of Different Planting Years Alfalfa[J].Northern Horticulture,2013,37(16):70.
[4]张秋萍,祁红英,张 珏,等.丛枝菌根制剂对黄瓜幼苗生长的影响[J].北方园艺,2014,38(14):37.
ZHANG Qiu-ping,QI Hong-ying,ZHANG Yu,et al.Effect of Arbuscular Mycorrhizal Agent on Growth of Cucumber Seedling[J].Northern Horticulture,2014,38(16):37.
[5]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(16):1.
[6]王昶童,曹刚强,梁丛敏,等.黄瓜NBS类抗病基因类似物的生物信息学分析[J].北方园艺,2014,38(14):101.
WANG Chang-tong,CAO Gang-qiang,LIANG Cong-min,et al.Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber[J].Northern Horticulture,2014,38(16):101.
[7]张瑞芬,李红岭,李新旭,等.优比胶囊不同使用方法对黄瓜生长及产量的影响[J].北方园艺,2013,37(17):4.
ZHANG Rui-fen,LI Hong-ling,LI Xin-xu,et al.Effect of Different Usage of UB Capsule on Growth and Yield of Cucumber[J].Northern Horticulture,2013,37(16):4.
[8]张雪艳,李堃,马丽娟,等.Ca2+调控对黄瓜幼苗耐盐性生理指标的影响[J].北方园艺,2013,37(13):5.
ZHANG Xue-yan,LI Kun,MA Li-juan,et al.Effects of Calcium Regulation on the Physiological Properties of Cucumber Under Salt Stress[J].Northern Horticulture,2013,37(16):5.
[9]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination，Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(16):9.
[10]张建祥,贾利元.越冬茬黄瓜结瓜期管理技术[J].北方园艺,2013,37(13):63.
ZHANG Jian-xiang,JIA Li-yuan.Management Techniques of Overwintering Cucumber During the Fruiting Period[J].Northern Horticulture,2013,37(16):63.
备注/Memo
第一作者简介：李笑月（1999-），女，硕士研究生，研究方向为黄瓜分子遗传育种。E-mail：xiaoyue7441@163.com.责任作者：辛明（1981-），男，博士，副研究员，硕士生导师，现主要从事黄瓜分子遗传育种等研究工作。E-mail：xinmingneau@126.com.基金项目：国家自然基金面上基金资助项目（32272724）；黑龙江省自然科学基金联合引导资助项目（LH2019C033）。收稿日期：2022-11-29