CAO Xiaoyan,YANG Haitao.Optimization Extraction of Polyphenols and its Antioxidant Activity From Ophiopogon japonicus Flowers Using Response Surface Methodology[J].Northern Horticulture,2019,43(02):1-6.[doi:10.11937/bfyy.20182247]
响应面法优化麦冬花多酚提取工艺及其抗氧化活性
- Title:
- Optimization Extraction of Polyphenols and its Antioxidant Activity From Ophiopogon japonicus Flowers Using Response Surface Methodology
- Keywords:
- Ophiopogon japonicus flowers; ultrasonic-assisted extraction; polyphenols; response surface methodology; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以采集于秦巴山区的野生麦冬根、叶及花为试材，采用单因素和响应面试验的方法，研究了超声辅助提取麦冬花多酚的提取工艺并评价了麦冬不同部位的多酚含量及其抗氧化功效，以期优化麦冬不同组织类多酚提取的工艺参数。结果表明：麦冬花多酚的最佳提取工艺条件为超声时间为31 min，提取温度为57 ℃，乙醇浓度为63%，料液比为1:19 g:mL-1，其多酚提取量为32.95 ± 0.14 mg·g-1，麦冬块根，叶及须根的多酚量分别为24.64 ± 0.05、20.52 ± 0.04、17.42 ± 0.03 mg·g-1。与常规抗氧化剂Vc对比，不同部位的麦冬多酚均具有较好地清除O2-·、·OH、NaNO2的能力，其总体清除活性顺序为麦冬花＞麦冬块根＞麦冬叶＞麦冬须根≥抗坏血酸；麦冬花多酚对O2-?、·OH和NaNO2的清除IC50值分别为0.41 ± 0.01、0.097 ± 0.005、0.021 ± 0.005 mg·mL-1，而同浓度条件下Vc溶液的清除IC50值分别为0.80 ± 0.01、0.14 ± 0.01、0.012 ± 0.002 mg·mL-1，表明不同部位的麦冬多酚均具有相对较强的抗氧化活性。
- Abstract:
- With single factor and response surface method, this study investigated the optimal process parameters for ultrasonic-assisted extracting total polyphenols from Ophiopogon japonicus flowers, which was sampled from the Qinba Mountains. Meanwhile, the scavenging effects of polyphenols from different parts of Ophiopogon japonicus on superoxide anion radical(O2-?), hydroxyl free radical(?OH) and nitrite were also examined. The results showed that the optimum condition for ultrasonic-assisted extracting of polyphenols from Ophiopogon japonicus flowers were as follows: extraction temperature 57 ℃, ethanol concentration 63%, ultrasonic time 31 minutes, ratio of material to liquid 1:19 g·mL-1). Under this condition, the content of polyphenols from Ophiopogon japonicus flowers was 32.95±0.14 mg·g-1. The polyphenols extraction contents from Ophiopogon japonicus tuber roots, leaf and fibrous root were 24.64 ± 0.05 mg·g-1, 20.52 ± 0.04 mg·g-1and 17.42 ± 0.03 mg·g-1, respectively. Comparing with conventional antioxidant ascorbic acid, total polyphenols from different parts of Ophiopogon japonicas extraction could be better removing superoxide anion radical(O2-?), hydroxyl free radical(?OH) and nitrite, and the order of scavenging free radicals was as follows: Ophiopogon japonicus flowers>tuberous root>leaf>fibrous root. The polyphenols from Ophiopogon japonicus flowers had certain scavenging ability on O2-?, ·OH and NaNO2 with IC50 of 0.41 ± 0.01 mg·mL-1, 0.097 ± 0.005 mg·mL-1and 0.021 ± 0.005 mg·mL-1 respectively, and scavenging free radical IC50 of Vc at the same concentration for O2-?, ·OH and NaNO2 were 0.80 ± 0.01 mg·mL-1, 0.14 ± 0.01 mg·mL-1 and 0.012 ± 0.002 mg·mL-1, respectively. The study proved that polyphenols from different parts of Ophiopogon japonicus extraction had relatively good antioxidant activity.
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CAO Xiaoyan,YANG Haitao.Optimization Extraction of Polyphenols and Its Antioxidant Activity From Ophiopogon japonicus Flowers Using Response Surface Methodology[J].Northern Horticulture,2019,43(02):130.[doi:10.11937/bfyy.20182247]
备注/Memo
第一作者简介：曹小燕(1982-)，女，博士，讲师，研究方向为天然产物提取及仿生合成。E-mail:ruoshuizhihan@163.com.基金：陕西理工大学校级科研资助项目（SLG1811）。收稿日期：2018―09―20