YANG Weijun,YU Zishen,SUN Hao,et al.Alleviating Effect of Exogenous ALA on Seed Germination and SeedlingGrowth of Prunella vulgaris Under Acid Stress[J].Northern Horticulture,2026,(17):90-99.[doi:10.11937/bfyy.20261016]
外源ALA对酸胁迫下夏枯草种子萌发及幼苗生长的缓解效应
- Title:
- Alleviating Effect of Exogenous ALA on Seed Germination and SeedlingGrowth of Prunella vulgaris Under Acid Stress
- 文章编号:
- 1001-0009(2026)17-0090-10
- Keywords:
- Prunella vulgaris; ALA; acid stress; seed germination; seedling growth; principal component analysis
- 分类号:
- S68
- 文献标志码:
- A
- 摘要:
- 以夏枯草种子为试材，采用 pH 3.0 混合酸液模拟酸雨胁迫，采用pH 3.0混合酸液模拟酸雨胁迫，并用0.1、0.5、1.0、5.0、10.0 mg·L-1的5-氨基乙酰丙酸（ALA）进行浸种处理，研究了外源ALA对酸胁迫环境中夏枯草种子萌发与幼苗生长的影响，以期为酸雨地区中药材栽培提供参考依据。结果表明：pH 3.0酸胁迫显著抑制夏枯草种子萌发与幼苗生长，导致发芽率、活力指数下降及细胞膜透性增大；外源ALA浸种在酸胁迫下显著提高夏枯草种子萌发和幼苗生长各项指标，其萌发与色素相关指标均随ALA浓度的增加先升高后下降，而相对电导率则呈相反变化。相关性分析结果表明，萌发、生长及色素指标间多呈极显著正相关，且与相对电导率呈极显著负相关。主成分分析显示ALA缓解效应排序为1.0 mg·L-1＞0.5 mg·L-1＞5.0 mg·L-1＞0.1 mg·L-1＞10.0 mg·L-1。综上，适宜浓度（1.0 mg·L-1）的ALA浸种可通过降低细胞膜透性、促进光合色素合成以及优先保障根系生长，有效减轻酸胁迫对夏枯草种子萌发及幼苗生长的抑制效应。
- Abstract:
- Taking Prunella vulgaris seeds as test materials,acid rain stress was simulated with a mixed acid solution at pH 3.0,and the seeds were soaked in 0.1,0.5,1.0,5.0,and 10.0 mg·L-1 5-aminolevulinic acid (ALA).The effects of exogenous ALA on seed germination and seedling growth of P.vulgaris under acid stress were investigated,in order to provide a reference for the cultivation of medicinal plants in acid rain areas.The results showed that the seed germination and seedling growth of P.vulgaris were significantly inhibited by the pH 3.0 acid stress,leading to a decrease in the germination rate and vigor index,as well as an increase in cell membrane permeability.Under acid stress,various indices of seed germination and seedling growth were significantly improved by exogenous ALA seed soaking.The germination and pigment related indices were observed to initially increase and then decrease with the increment of ALA concentration,whereas an opposite trend was exhibited by the relative electrical conductivity.Based on correlation analysis,highly significant positive correlations were mostly found among the germination,growth,and pigment indices,while highly significant negative correlations were observed between these indices and the relative electrical conductivity.The alleviating effects of ALA treatments were ranked by principal component analysis in the following order:1.0 mg·L-1 > 0.5 mg·L-1 > 5.0 mg·L-1 > 0.1 mg·L-1 > 10.0 mg·L-1.In conclusion,the inhibitory effects of acid stress on the seed germination and seedling growth of P.vulgaris were effectively alleviated by seed soaking with an appropriate concentration of ALA (1.0 mg·L-1).This alleviation was achieved through the reduction of cell membrane permeability,the promotion of photosynthetic pigment synthesis,and the prioritized protection of root growth.
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备注/Memo
第一作者简介：杨卫军（1977-），男，硕士，讲师，现主要从事园艺栽培等研究工作。E-mail:hnayywj2016@163.com.责任作者：常青山（1979-），男，博士，副教授，现主要从事植物逆境生理与作物品质调控等研究工作。E-mail:hkdcqs@126.com.基金项目：国家自然科学基金资助项目（31800096）；河南省重点攻关资助项目（252102110148）；河南省自然科学基金资助项目（252300423035）；安阳工学院太行山林草资源保护与利用协同创新中心资助项目（XTCX202402）；大学生创新创业训练计划资助项目(2025463)。收稿日期：2026-03-31