[1]李艳,孙雪雨,胡体旭,等.番茄抗坏血酸合成基因SlGPP2克隆及功能分析[J].北方园艺,2026,(17):45-55.[doi:10.11937/bfyy.20260605]
　LI Yan,SUN Xueyu,HU Tixu,et al.Cloning and Functional Analysis of the Tomato Ascorbic Acid Synthesis Gene SlGPP2[J].Northern Horticulture,2026,(17):45-55.[doi:10.11937/bfyy.20260605]
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番茄抗坏血酸合成基因SlGPP2克隆及功能分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年17 页码: 45-55 栏目: 出版日期: 2026-09-15
Title:
Cloning and Functional Analysis of the Tomato Ascorbic Acid Synthesis Gene SlGPP2
文章编号:
1001-0009(2026)17-0045-11
作者:
李艳1; 孙雪雨1; 胡体旭2; 赵红星1; 陈宏1; 孙联合1
(1.驻马店市农业科学院，河南 驻马店 463000；2.西北农林科技大学 园艺学院，陕西 杨凌 712100)
Author(s):
LI Yan1; SUN Xueyu1; HU Tixu2; ZHAO Hongxing1; CHEN Hong1; SUN Lianhe1
(1.Zhumadian Agricultural Academy of Sciences,Zhumadian,Henan 463000;2.College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100)
关键词:
番茄; 抗坏血酸; 生物合成; 基因功能
Keywords:
tomato; ascorbic acid; biosynthesis; gene function
分类号:
S641.1
DOI:
10.11937/bfyy.20260605
文献标志码:
A
摘要:
以番茄品系AC为试材，采用基因克隆、生物信息学分析及遗传转化等方法，研究了SLGPP2基因对番茄抗坏血酸（AsA）合成途径相关基因表达的影响，以期为揭示SLGPP2在番茄AsA合成中的分子机制提供参考依据。结果表明：SLGPP2基因cDNA全长807 bp，编码268个氨基酸。系统进化分析显示，SLGPP2与曼陀罗L-半乳糖-1-磷酸磷酸酶（GPP）蛋白亲缘关系较近，与其他物种GPP蛋白序列相似性为60.51%。表达模式分析显示，SLGPP2在磷酸组织中均有表达，呈明显组织特异性，且花中表达量最高。功能鉴定结果表明，超量表达SLGPP2可显著提升番茄幼叶和破色期果实的AsA含量，增幅最高分别达61.01%和94.32%；RNAi沉默SLGPP2则显著降低叶片和果实中AsA含量，降幅最高分别达69.05%和42.03%，且SLGPP2通过影响AsA合成途径相关基因的表达，进而改变番茄AsA含量。
Abstract:
Taking the tomato strain AC as the test material,through methods such as gene cloning,bioinformatics analysis and genetic transformation,the influence of the SlGPP2 gene on the expression of genes related to ascorbic acid (AsA) synthesis pathway in tomato was studied,in order to provide references for revealing the molecular mechanism of SlGPP2 in tomato AsA synthesis.The results showed that the full-length cDNA of SlGPP2 was 807 bp,encoding 268 amino acids.Systematic evolutionary analysis indicated that SlGPP2 had a closer genetic relationship with the datura stramonium GPP protein,and the similarity of its sequence to GPP proteins of other species was 60.51%.Expression pattern analysis revealed that SlGPP2 was expressed in all tissues of tomato,with obvious tissue specificity,and the expression level was the highest in flowers.Functional identification results showed that overexpression of SlGPP2 could significantly increase the AsA content in young leaves and senescing fruits of tomato,with the maximum increase reaching 61.01% and 94.32% respectively;RNAi silencing of SlGPP2 significantly reduced the AsA content in leaves and fruits,with the maximum reduction reaching 69.05% and 42.03% respectively.Moreover,SlGPP2 changed the AsA content of tomato by affecting the expression of genes related to the AsA synthesis pathway.

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备注/Memo

第一作者简介：李艳（1986-），女，硕士，副研究员，现主要从事园艺作物遗传育种与栽培等研究工作。E-mail：ease1988@163.com.责任作者：孙联合（1975-），男，硕士，研究员，现主要从事作物遗传育种与技术推广等研究工作。E-mail：13938358765@163.com.基金项目：驻马店市重大科技专项资助项目（ZMDSZDZX2022005）。收稿日期：2026-02-25

更新日期/Last Update: 2026-09-17