SU Zhou,WANG Menghan,ZHAO Jinmeng,et al.Genome-wide Identification of the CNGC Gene Family and Expression Profiles Under Stress Conditions in Melon[J].Northern Horticulture,2026,(15):7-17.[doi:10.11937/bfyy.20260483]
甜瓜CNGC基因家族全基因组鉴定及胁迫表达模式分析
- Title:
- Genome-wide Identification of the CNGC Gene Family and Expression Profiles Under Stress Conditions in Melon
- 文章编号:
- 1001-0009(2026)15-0007-11
- Keywords:
- Cucumis melo L.; CNGC gene family; whole genome; abiotic stress
- 分类号:
- S652
- 文献标志码:
- A
- 摘要:
- 以甜瓜(Cucumis melo L.)为试材,采用生物信息学和分子生物学的研究方法,对甜瓜CNGC基因家族成员进行全基因组鉴定和非生物胁迫下的调控模式分析,以期为解析CNGC基因家族在甜瓜应对非生物胁迫中的功能提供参考依据。结果表明:甜瓜基因组中有16个CmCNGCs基因,不均匀分布在8条染色体上,分属为4个亚族,共包含15个Motif,其启动子区域存在多种响应逆境胁迫的作用元件,暗示CmCNGCs在响应非生物胁迫中可能存在重要调控作用。多种非生物胁迫诱导表达分析表明,大多数 CmCNGCs基因表现出明显的差异表达。
- Abstract:
- Melon (Cucumis melo L.) was used as the experimental material. Genome-wide identification of the CNGC gene family members and analysis of the regulatory patterns under abiotic stress were performed using bioinformatics and molecular biology approaches.in order to provide a reference for elucidating the functions of the CNGC gene family in melons under abiotic stress.The results showed that there were 16 CmCNGC genes unevenly distributed across 8 chromosomes in the melon genome,which were classified into four subfamilies and contain 15 conserved Motifs.The promoter regions of these genes included multiple cis-acting elements responsive to stress,suggesting that CmCNGC genes may play important regulatory roles in abiotic stress responses.Expression analysis under various abiotic stresses indicated that most CmCNGC genes exhibited distinct differential expression.
参考文献/References:
[1]HAO L,QIAO X.Genome-wide identification and analysis of the CNGC gene family in maize[J].PeerJ,2018,6:e5816.[2]SCHUURINK R C,SHARTZER S F,FATH A,et al.Characterization of a calmodulin-binding transporter from the plasma membrane of barley aleurone[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(4):1944-1949.[3]WANG J,DU B Y,ZHANG X,et al.Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity[J].Nature Plants,2025,11(3):632-642.[4]SUN Y,GAO L,HAN Y,et al.Activation of the CNGC2-CNGC4 channel complex by P2K1-mediated phosphorylation links extracellular ATP perception to calcium signaling in plant immunity[J].Molecular Plant,2025,18(7):1130-1142.[5]SAAND M A,XU Y P,LI W,et al.Cyclic nucleotide gated channel gene family in tomato:Genome-wide identification and functional analyses in disease resistance[J].Frontiers in Plant Science,2015,6:303.[6]王田田,常雪瑞,王静.辣椒CNGC基因家族鉴定及表达分析[J].农业生物技术学报,2025,33(2):271-284.[7]JIANG Z,DU L,SHEN L,et al.Genome-wide exploration and expression analysis of the CNGC gene family in eggplant (Solanum melongena L.) under cold stress,with functional characterization of SmCNGC1a[J].International Journal of Molecular Sciences,2023,24(17):13049.[8]KAKAR K U,NAWAZ Z,KAKAR K,et al.Comprehensive genomic analysis of the CNGC gene family in Brassica oleracea:Novel insights into synteny,structures,and transcript profiles[J].BMC Genomics,2017,18(1):869.[9]LI Q,YANG S,REN J,et al.Genome-wide identification and functional analysis of the cyclic nucleotide-gated channel gene family in Chinese cabbage[J].3 Biotech,2019,9(3):114.[10]常静静.西瓜褪黑素合成基因鉴定及褪黑素调控抗冷性的信号传导机制[D].杨凌:西北农林科技大学,2021.[11]LIU J,WANG Y,PENG L,et al.Genome-wide identification of the cyclic nucleotide-gated ion channel gene family and expression profiles under low-temperature stress in Luffa cylindrica L.[J].International Journal of Molecular Sciences,2024,25(20):11330.[12]WANG L,LI M,LIU Z,et al.Genome-wide identification of CNGC genes in Chinese jujube (Ziziphus jujuba Mill.) and ZjCNGC2 mediated signalling cascades in response to cold stress[J].BMC Genomics,2020,21(1):191.[13]ZHANG Y,LI Y,YANG J,et al.Genome-wide analysis and expression of cyclic nucleotide-gated ion channel (CNGC) family genes under cold stress in mango(Mangifera indica)[J].Plants,2023,12(3):592.[14]ZIA K,RAO M J,SADAQAT M,et al.Pangenome-wide analysis of cyclic nucleotide-gated channel (CNGC) gene family in Citrus Spp.Revealed their intraspecies diversity and potential roles in abiotic stress tolerance[J].Frontiers in Genetics,2022,13:1034921.[15]彭丽娟.外源钙对普通丝瓜低温胁迫的缓解作用及(Ca2+)通道相关基因CNGC分析[D].福州:福建农林大学,2025.[16]SHI J,DU X.Transcriptome analysis reveals the regulation of cyclic nucleotide-gated ion channels in response to exogenous abscisic acid and calcium treatment under drought stress in tomato[J].Frontiers in Genetics,2023,14:1139087.[17]刘玉丰,闫征南,万泽,等.盐碱胁迫下黄腐酸对甜瓜幼苗生长抑制的缓解效应[J].北方园艺,2021(24):45-50.[18]周晓健,高国正,刘星,等.甜瓜叶片芽黄自交系杂种优势及配合力分析[J].北方园艺,2025(10):1-9.[19]CHEN C,CHEN H,ZHANG Y,et al.TBtools:An integrative toolkit developed for interactive analyses of big biological data[J].Molecular Plant,2020,13(8):1194-1202.[20]黄超.不同甜瓜品种在低温胁迫下转录组分析[D].哈尔滨:东北农业大学,2018.[21]WANG L M,ZHANG L D,CHEN J B,et al.Physiological analysis and transcriptome comparison of two muskmelon (Cucumis melo L.) cultivars in response to salt stress[J].Genetics and Molecular Research,2016,15(3):15038738.[22]杨帆,邹昌利,张利超,等.甜瓜CmLEA基因家族鉴定及抗非生物胁迫基因挖掘[J].南方农业学报,2025,56(2):563-572.[23]章丽珍,韩晓云,吴菁华,等.甜瓜实时荧光定量PCR分析中内参基因的筛选[J].福建农业学报,2020,35(11):1179-1187.[24]王雪莹,王瑞琪,张洋,等.毛果杨CNGC家族全基因组鉴定及胁迫响应分析[J].植物研究,2022,42(4):613-625.[25]ZELMAN A K,DAWE A,GEHRING C,et al.Evolutionary and structural perspectives of plant cyclic nucleotide-gated cation channels[J].Frontiers in Plant Science,2012,3:95.[26]张倩,任海林,王洁,等.猕猴桃FBA基因家族的鉴定及在干旱胁迫下的表达模式分析[J].北方园艺,2025(23):20-29.[27]石雅琦,刘海双,柯瑾,等.植物环核苷酸门控离子通道研究进展[J].植物学报,2025,60(2):294-306.[28]刘琳,秦于玲,傅慧珍,等.辣椒NAM基因家族的全基因组鉴定及其在果实成熟期表达分析[J/OL].热带作物学报,2026,47(5):1132-1143.[29]李小红,王晓彤,麻旭霞,等.紫花苜蓿CNGC基因家族成员鉴定及分析[J].草地学报,2024,32(2):588-598.[30]沈悦,沈一,刘永惠,等.花生〖STBX〗AhGPAT9〖STBZ〗基因启动子克隆及其功能分析[J].中国油料作物学报,2023,45(3):533-541.[31]杨勇,闫俊峰,杨伟民,等.羽衣甘蓝〖STBX〗BoNAC02〖STBZ〗基因参与脱落酸和干旱胁迫响应的功能研究[J].北方园艺,2025(23):1-10.[32]ORANAB S,GHAFFAR A,KIRAN S,et al.Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 in Arabidopsis thaliana for their potential role in salt stress[J].Saudi Journal of Biological Sciences,2021,28(10):5800-5807.[33]CUI Y,LU S,LI Z,et al.CYCLIC NUCLEOTIDE-GATED ION CHANNELs 14 and 16 promote tolerance to heat and chilling in rice[J].Plant Physiology,2020,183(4):1794-1808.[34]WANG X,WU F,ZHANG J,et al.Identification of the CNGC gene family in rice and mining of alleles for application in rice improvement[J].Plants,2023,12(24):4089.
相似文献/References:
[1]王晓静,马文平,王芳.甜瓜采后致病菌交链菌的防治方法研究[J].北方园艺,2014,38(04):103.
WANG Xiao-jing,MA Wen-ping,WANG Fang.Study on Prevention Method of Alternariasp. After Melon Postharvest[J].Northern Horticulture,2014,38(15):103.
[2]宁雪飞,韩小路,王贤磊,等.一种快速筛选转抗霜霉病基因甜瓜种子的方法研究[J].北方园艺,2014,38(01):102.
NING Xue-fei,HAN Xiao-lu,WANG Xian-lei,et al.The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon[J].Northern Horticulture,2014,38(15):102.
[3]王晓梅,刘国宁,程丽,等.部分黄瓜和甜瓜品种对霜霉病的抗性及其组分研究[J].北方园艺,2014,38(04):106.
WANG Xiao-mei,LIU Guo-ning,CHENG Li,et al.Study on Resistance to Downy Mildew of Some Cucumber and Melon Varieties and Its Component[J].Northern Horticulture,2014,38(15):106.
[4]张君艳,罗爱玉,张君君.河西地区杂交甜瓜大田制种生产技术[J].北方园艺,2013,37(14):62.
[J].Northern Horticulture,2013,37(15):62.
[5]苏艳,王盼乔,胡建斌.十五份甜瓜种质苗期性状的聚类分析及相关分析[J].北方园艺,2014,38(07):5.
SU Yan,WANG Pan-qiao,HU Jian-bin.Clustering and Correlation Analysis of 15 Cucumis melo L.Germplasms Based on the Phenotypic Traits of the Seedling Period[J].Northern Horticulture,2014,38(15):5.
[6]赵九洲,胡立盼,徐志然,等.甜瓜幼苗耐盐碱性及缓解盐碱胁迫γ-氨基丁酸浓度的筛选[J].北方园艺,2014,38(09):1.
ZHAO Jiu-zhou,HU Li-pan,XU Zhi-ran,et al.The Screening of Melon(Cucumis melo L.)Seedling Cultivars for Salt-alkaline Tolerance and the Concentration of γ-aminobutyric Acid Alleviating Salt-alkaline Stress[J].Northern Horticulture,2014,38(15):1.
[7]王喜涛,周秀艳,辛 明,等.盐胁迫对甜瓜种子发芽的影响[J].北方园艺,2014,38(09):7.
WANG Xi-tao,ZHOU Xiu-yan,XIN Ming,et al.Effect of NaCl Stress on Seed Germination of Melon[J].Northern Horticulture,2014,38(15):7.
[8]宋 羽,卢青成,王 浩.适合新疆戈壁地区甜瓜穴盘育苗的基质相对含水量的探究[J].北方园艺,2014,38(09):53.
SONG Yu,LU Qing-cheng,WANG Hao.Research on Suitable Substrate Water Content for Melon Plug-seedling in Gobi District of Xinjiang[J].Northern Horticulture,2014,38(15):53.
[9]苏丽影,宋述尧,赵春波,等.玉米秸秆混合基质对甜瓜穴盘苗生长的影响[J].北方园艺,2013,37(12):28.
SU Li-ying,SONG Shu-yao,ZHAO Chun-bo,et al.Effect of Mixed Substrates of Corn Stalk on Plug Seeding of Muskmelon[J].Northern Horticulture,2013,37(15):28.
[10]王爱玲,张敏,廖新福,等.甜瓜“黄醉仙”下胚轴再生体系的初步建立[J].北方园艺,2013,37(12):100.
WANG Ai-ling,ZHANG Min,LIAO Xin-fu,et al.Preliminary Establishment of Melon cv.‘Huangzuixian’ Regeneration System[J].Northern Horticulture,2013,37(15):100.
备注/Memo
第一作者简介:苏洲(1999-),男,硕士研究生,研究方向为甜瓜分子遗传育种。E-mail:1612046103@qq.com.责任作者:刘宏宇(1967-),男,博士,副研究员,现主要从事甜瓜分子遗传育种等研究工作。E-mail:hyliu@neau.edu.cn.基金项目:黑龙江省自然科学基金资助项目(LH2021C034);国家西甜瓜产业技术体系资助项目(CARS-025-G9)。收稿日期:2026-02-04