[1]王鹏程,赵书雪,张恩建,等.长根菇种质抗木霉性状评价[J].北方园艺,2026,(15):115-123.[doi:10.11937/bfyy.20260331]
　WANG Pengcheng,ZHAO Shuxue,ZHANG Enjian,et al.Evaluation of Trichoderma Resistance Trait in Germplasm of Oudemansiella raphanipies[J].Northern Horticulture,2026,(15):115-123.[doi:10.11937/bfyy.20260331]
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长根菇种质抗木霉性状评价

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年15 页码: 115-123 栏目: 出版日期: 2026-08-15
Title:
Evaluation of Trichoderma Resistance Trait in Germplasm of Oudemansiella raphanipies
文章编号:
1001-0009(2026)15-0115-09
作者:
王鹏程1; 赵书雪1; 张恩建2; 孙然2; 史灵燕1; 李维焕1; 3
（1.鲁东大学 园艺学院,烟台市食药用菌技术创新中心,现代种业与绿色种养研究中心,山东 烟台 264025；2.金乡县联盛菌业科技有限公司,山东 济宁 272209；3.国家盐碱地综合利用技术创新中心，山东 东营，257345）
Author(s):
WANG Pengcheng1; ZHAO Shuxue1; ZHANG Enjian2; SUN Ran2; SHI Lingyan1; LI Weihuan1; 3
(1.College of Horticulture,Ludong University/Yantai Technology Innovation Center of Edible and Medicinal Fungi/Research Center of Modern Seed Industry and Green Crop-Livestock Farming,Yantai,Shandong 264025;2.Jinxiang Liansheng Edible Fungi Technology Co.,Ltd.,Jining,Shandong 272209;3.National Technology Innovation Center for Comprehensive Utilization of Saline-Alkali Land,Dongying,Shandong 257345)
关键词:
长根菇; 木霉; 筛选; 拮抗
Keywords:
Oudemansiella raphanipies; Trichoderma spp.; screening; antagonism
分类号:
S646
DOI:
10.11937/bfyy.20260331
文献标志码:
A
摘要:
以26株长根菇菌株为试材，采用平板对峙培养方法，研究了木霉对长根菇生长的影响，筛选出抗木霉特性优异的长根菇优良菌株，以期为长根菇栽培提供参考依据。结果表明：供试26株长根菇菌株的生长势存在显著种内差异，其中菌株3、4、5、6、10、12、13、14、15、16、20、22、24、25、26、27、28菌落形态整齐，菌丝浓密粗壮，生长势表现强劲；菌株4、15、24为生长速率最快的类群。从污染菌棒中成功分离鉴定出1株长根菇栽培中的优势污染木霉菌株，经鉴定为哈茨木霉，命名为LV1。平板对峙培养结果表明，不同长根菇菌株对哈茨木霉LV1的抗性存在显著差异，其中菌株3、5、6、9、13、14表现出优异的抗LV1能力，为高抗菌株。该研究筛选获得的长根菇高抗木霉菌株，可直接应用于长根菇规模化栽培生产，为有效解决生产实践中的木霉污染难题提供了优质菌株资源与技术支撑。
Abstract:
Taking 26 strains of Oudemansiella raphanipies as test materials,and the plate confrontation culture method was employed to investigate the effects of Trichoderma spp.on the growth of O.raphanipies,aiming to screen excellent O.raphanipies strains with superior Trichoderma spp.resistance,in order to provide a reference for its cultivation.The results showed that there were significant intraspecific differences in the growth vigor among the 26 tested O.raphanipies strains.Among them,strains 3,4,5,6,10,12,13,14,15,16,20,22,24,25,26,27,and 28 exhibited neat colony morphology,dense and robust mycelia,and strong growth vigor;strains 4,15,and 24 constituted the group with the fastest growth rate.One dominant contaminating Trichoderma spp.strain in O.raphanipies cultivation was successfully isolated and identified from the contaminated fungus sticks,which was identified as Trichoderma harzianum and designated as LV1.The results of plate confrontation culture indicated that there were significant differences in the resistance of different O.raphanipies strains to LV1,among which strains 3,5,6,9,13 and 14 showed excellent resistance to LV1 and were identified as highly resistant strains.The O.raphanipies strains with high Trichoderma spp.resistance screened in this study can be directly applied to the large-scale cultivation of O.raphanipies,which provides high-quality strain resources and technical support for effectively solving the problem of Trichoderma spp.contamination in production practice.

参考文献/References:

［1］钟楚梦,吴一凡,陈胜,等.长根菇竹林下栽培研究及其对土壤理化性质的影响［J］.食用菌,2024,46(6):40-44.［2］肖自添,何焕清,刘明,等.卵孢小奥德蘑栽培发展历程及技术要点［J］.食药用菌,2022,30(4):277-282.［3］卢彩会,郭晓萌,牟德华.黑皮鸡枞菌水提物和醇提物的主要成分及抗氧化性研究［J］.食品工程,2016(3):35-40,57.［4］崔晓,丛倩倩,王庆武,等.无机盐、维生素及生长调节剂对卵孢长根菇菌丝的影响［J］.北方园艺,2024(9):110-116.［5］万鲁长,任海霞,郭惠东,等.长根菇霉菌重度污染分析及其绿色防控关键技术［J］.食用菌,2021,43(5):66-68.［6］万鲁长,任海霞,任鹏飞,等.长根菇控温菇房周年化立体栽培关键技术［J］.食用菌,2020,42(1):49-50,53.［7］王森.原生质体单核化杂交选育卵孢小奥德蘑抗木霉新菌株［D］.保定:河北农业大学,2022.［8］程旭,隋哲,田朋姣,等.几种植物醇提物对血耳木霉病原菌抑菌试验［J］.食用菌,2024,46(1):67-70.［9］吴小平.食用菌致病木霉的鉴定、致病机理及防治研究［D］.福州:福建农林大学,2008.［10］陈艳露,周莉,黄麟淇,等.抗木霉平菇菌株的筛选及其生产性能评价［J］.北方园艺,2013(17):145-148.［11］刘昆昂,张根伟,马宏,等.抗木霉白色金针菇菌株的选育及其栽培配方［J］.北方园艺,2019(1):150-157.［12］王增术.秀珍菇烂袋原因及其预防措施［J］.食用菌,2004,26(5):43-44.［13］陈天泰,马福义.食用菌绿霉病综合防治技术［J］.食用菌,2004,26(5):44-45.［14］兰清秀,柯斌榕,卢政辉,等.不同温度和培养基对秀珍菇木霉共培养的影响［J］.福建农业学报,2022,37(2):197-202.［15］唐义恒,程单单,徐涛,等.红托竹荪腐烂病病原真菌鉴定及药剂敏感性研究［J］.北方园艺,2026(6):91-100.［16］解宜林,檀根甲.灵芝绿霉病发病原因分析及防治措施［J］.中国食用菌,2015,34(1):74-76.［17］吕骐羽,张秀停,宫玉柱,等.卵孢小奥德蘑抗哈茨木霉菌株的筛选研究［J］.北方园艺,2024(15):118-127.［18］ZHAO S,LI X,YAO X,et al.Detoxification of tetracycline and synthetic dyes by a newly characterized Lentinula edodes laccase,and safety assessment using proteomic analysis［J］.Ecotoxicology and Environmental Safety,2024,276:116324.［19］陈薪全,常康,何定坤,等.酢浆草鳞茎内生菌的多样性分析与固氮、解磷、解钾功能菌株分离鉴定［J］.微生物学通报,2025,52(10):4577-4604.［20］郭鹏豪,刘秀丽,崔颖鹏,等.真菌通用引物Its1和Its4在丝状真菌鉴定中的价值评价［J］.中国微生态学杂志,2013,25(8):922-924.［21］李晓.香菇优良杂交新菌株选育的研究［D］.长春:吉林农业大学,2002.［22］熊小龙.四川金针菇木霉病害的研究及金针菇菌株抗性分析［D］.雅安:四川农业大学,2011.［23］桑峰.木耳优良杂交菌株的初步筛选［D］.武汉:华中农业大学,2011.［24］阳丽坪.香菇种质抗木霉性状评价及抗性基因挖掘［D］.武汉:华中农业大学,2024.［25］李辉平,朱家漘,林金盛,等.42个草菇栽培菌株对绿色木霉和腐食酪螨的抗性鉴定［J］.食药用菌,2024,32(1):47-51.［26］贺黎铭,蔡凌,谢欣,等.灵芝木霉病病原菌分离鉴定及抗病种质筛选［J］.北方园艺,2025(2):59-66.

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备注/Memo

第一作者简介：王鹏程（1998-），男，硕士研究生，研究方向为食用菌遗传育种。E-mail：19861622099@163.com.责任作者：李维焕（1979-），女，博士，副教授，现主要从事食用菌遗传育种、栽培和生理等研究工作。E-mail：lwh1969@163.com.基金项目：济宁市重点研发计划资助项目（2023NYNS005）；山东省现代农业产业技术体系食用菌创新团队遗传育种岗位资助项目(SDAIT-07-03)。收稿日期：2026-01-27

更新日期/Last Update: 2026-08-14