WEN Jie,SONG Yiming,AN Huaming,et al.Analysis of Triterpenoid Monomer Contents and Screening of Superior Germplasm in 47 Accessions of Rosa roxburghii[J].Northern Horticulture,2026,(17):19-27.[doi:10.11937/bfyy.20260300]
四十七份刺梨种质资源三萜单体含量分析与优异种质筛选
- Title:
- Analysis of Triterpenoid Monomer Contents and Screening of Superior Germplasm in 47 Accessions of Rosa roxburghii
- 文章编号:
- 1001-0009(2026)17-0019-09
- 分类号:
- S661.9
- 文献标志码:
- A
- 摘要:
- 以47份刺梨种质（含10份核心种质和37份‘GN5’经60Co-γ射线辐照与EMS化学诱变获得的诱变种质）为试材，采用高效液相色谱（HPLC）法测定果实中蔷薇酸、委陵菜酸、刺梨苷和野蔷薇苷4种三萜单体的含量，并结合统计分析与系统聚类方法，研究了不同刺梨种质间的遗传变异，以期评价其遗传变异并筛选优异种质。结果表明：蔷薇酸与刺梨苷为主要组成成分，平均含量分别为3.823 mg·g-1与3.699 mg·g-1，4种单体含量变异系数介于24.59%~39.07%，其中野蔷薇苷和委陵菜酸变异系数较高，且各单体含量均呈正态分布；诱变处理显著改变了‘GN5’三萜单体的含量与组成比例，诱变群体蔷薇酸和委陵菜酸平均含量较原始种质分别提升1.024 mg·g-1和0.168 mg·g-1，对应占比增加14.15个和2.44个百分点，而刺梨苷和野蔷薇苷含量及占比显著降低；聚类分析将种质划分为5个类群，包括4类单体综合高含量的第Ⅰ类、蔷薇酸与委陵菜酸特异高含量的第Ⅴ类，以及刺梨苷与野蔷薇苷双高的第Ⅲ类等。该研究筛选出的‘RrY24’‘RrY9’‘RrY8’和‘GN5’等优特异材料，证实诱变可有效调控刺梨三萜单体积累，筛选的优异种质及划分的功能类群为刺梨三萜定向开发、种质创新及新品种培育提供了重要参考依据。
- Abstract:
- This study utilized 47 accessions of Rosa roxburghii,comprising 10 core germplasms and 37 mutant lines derived from ‘GN5’ through 60Co-γ irradiation and EMS chemical mutagenesis.High-performance liquid chromatography (HPLC) was employed to determine the content of four triterpene monomers，euscaphic acid,tormentic acid,kajiichigoside F1,and rosamultin in the fruit.Combined with statistical analysis and systematic clustering methods,the genetic variation among different Rosa roxburghii accessions was investigated,in order to evaluate their genetic diversity and screening for superior accessions.The results showed that the significant differences in the contents of the four triterpenoid monomers,with euscaphic acid and kajiichigoside F1 being the predominant components,averaging 3.823 mg·g-1 and 3.699 mg·g-1,respectively.The coefficients of variation for the four monomers ranged from 24.59% to 39.07%,with rosamultin and tormentic acid exhibiting higher variability,while all monomer contents followed a normal distribution.Mutagenic treatment significantly altered the content and compositional proportions of triterpenoid monomers in ‘GN5’.In the mutant population,the average contents of euscaphic acid and tormentic acid increased by 1.024 mg·g-1 and 0.168 mg·g-1,respectively,compared to the original germplasm,leading to relative proportion increases of 14.15 and 2.44 percentage points.Conversely,the contents and proportional shares of kajiichigoside F1 and rosamultin were significantly reduced.Cluster analysis categorized the germplasms into five groups,Group I,characterized by comprehensively high content of all four monomers;Group V,distinguished by specifically high content of euscaphic acid and tormentic acid;and Group Ⅲ,notable for high content of both kajiichigoside F1 and rosamultin.The superior materials identified in this study,including ‘RrY24’‘RrY9’‘RrY8’,and ‘GN5’,had demonstrated the genetic diversity of R.roxburghii triterpene monomers.It had been confirmed that mutagenesis could effectively regulate the accumulation of triterpene monomers in R.roxburghii.The excellent germplasms identified and the classified functional groups provide a crucial reference for the targeted development of R.roxburghii triterpenes,germplasm innovation,and the cultivation of new varieties.
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备注/Memo
第一作者简介：文洁（1999-），女，硕士研究生，研究方向为果树分子生物学。E-mail:jwen1126@163.com.责任作者：南红（1985-），女，博士，讲师，硕士生导师，现主要从事果树分子生物学、比较基因组学和大数据分析等研究工作。E-mail:hnan@gzu.edu.cn.基金项目：贵州省科技计划资助项目（黔科合基础-ZK［2022］一般092）;贵州省科技重大资助项目（黔科合重大［2025］048）。收稿日期：2026-01-23