TANG Tingting,JIN Rui,HUANG Xilun,et al.Antibacterial Activity and Growth-promoting Capability of Methylobacterium PjM1 in Panax japonicus Callus Tissue[J].Northern Horticulture,2026,(13):88-94.[doi:10.11937/bfyy.20254184]
竹节参愈伤组织中甲基杆菌PjM1的抑菌活性及促生能力研究
- Title:
- Antibacterial Activity and Growth-promoting Capability of Methylobacterium PjM1 in Panax japonicus Callus Tissue
- 文章编号:
- 1001-0009(2026)13-0088-07
- 分类号:
- S567.5
- 文献标志码:
- A
- 摘要:
- 以竹节参新鲜叶片为试材,采用组织分离法、革兰氏染色、16S rDNA测序、打孔法、拟南芥促生试验,研究了PjM1的生长培养基、分类、抑菌活性和促生潜能,以期为开发基于甲基杆菌的植物生长促进剂和生物防治剂提供参考依据。结果表明:甲基杆菌PjM1适合在MS、TSA、NA和PDA平板上生长,革兰氏染色和16S rDNA测序鉴定为甲基杆菌(Methylobacterium),将其命名为PjM1。筛选得到的PjM1对枯草芽孢杆菌有抑菌活性,平均抑菌圈直径为8 mm,对大肠杆菌和金黄色葡萄球菌均无抑菌活性;PjM1代谢产物对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均无抑菌活性;PjM1可以提高拟南芥种子萌发速度,对拟南芥幼苗主根和侧根数的发育和生长有促进作用,其中对主根的发育促进效果较为显著。接种菌株PjM1对拟南芥类胡萝卜素和叶绿素含量增加有显著影响。
- Abstract:
- Taking the fresh leaves of Panax japonicus as the test material,the growth medium,classification,antibacterial activity and promoting potential of PjM1 were studied by means of tissue separation method,Gram staining,16S rDNA sequencing,perforation method and Arabidopsis thaliana promotion test,in order to provide a reference for the development of plant growth promoters and biological control agents based on Methylobacterium.The results showed that Methylobacterium PjM1 grew well on MS,TSA,NA,and PDA agar plates.Gram staining and 16S rDNA sequencing identified the bacterium as belonging to the genus Methylobacterium and it was named PjM1.Screening revealed that PjM1 exhibited antibacterial activity against Bacillus subtilis,with an average zone of inhibition diameter of 8 mm.However,it showed no antibacterial activity against Escherichia coli or Staphylococcus aureus.Metabolites of PjM1 also exhibited no antibacterial activity against these three bacteria.PjM1 accelerated the germination rate of Arabidopsis thaliana seeds and promoted the development and growth of primary and lateral roots in Arabidopsis thaliana seedlings.It had a particularly significant effect on primary root development.Inoculation with the PjM1 strain significantly increased the carotenoid and chlorophyll content in Arabidopsis thaliana.
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备注/Memo
第一作者简介:唐婷婷(1999-),女,硕士研究生,研究方向为竹节参次生代谢产物调控。E-mail:2021172860@qq.com.责任作者:张来(1977-),男,博士,教授,硕士生导师,现主要从事竹节参次生代谢产物调控等研究工作。E-mail:Zhanglai1977725@126.com.基金项目:国家自然科学基金资助项目(31660252);贵州省科技厅科技资助项目(黔科合基础-ZK(2023)重点002);安顺学院研究生创新专项计划资助项目(asxyyjscx 202411)。收稿日期:2025-12-04