XIE Shuangyu,WANG Zhiwei,JING Qiuju,et al.Analysis of Changes in Nutrient Content in Blue Honeysukles at Different Colour Change Periods[J].Northern Horticulture,2026,(17):72-80.[doi:10.11937/bfyy.20254058]
不同转色时期蓝靛果营养成分的变化
- Title:
- Analysis of Changes in Nutrient Content in Blue Honeysukles at Different Colour Change Periods
- 文章编号:
- 1001-0009(2026)17-0072-09
- Keywords:
- blue honeysuckle; different colour change periods; physicochemical properties; nutritional composition
- 分类号:
- S663.9
- 文献标志码:
- A
- 摘要:
- 以不同转色时期蓝靛果为试材,采用高效液相色谱、电感耦合等离子质谱技术检测未转色期、转色前期、转色后期、完全转色期蓝靛果理化性质(百果质量、可溶性固形物、可滴定酸、pH)、营养成分(有机酸、糖、花青素、多酚、黄酮、矿物质、氨基酸)、抗氧化能力(DPPH自由基清除能力、ABTS自由基清除能力、羟自由基清除能力)的变化趋势,同时进行PLS-DA分析,并初步建立生理特征与蓝靛果果实成熟之间相关性的研究,研究其转色过程中果实理化性质与营养成分的变化,以期为蓝靛果的深入研究提供参考依据。结果表明:随着转色的进行,蓝靛果的百果质量无显著变化趋势,可溶性固形物、糖含量和抗氧化能力显著增加,总酸与有机酸含量显著降低,矿物质含量整体呈现先增加后降低的趋势,氨基酸含量呈现先降低后增加的趋势,同时这些理化指标与营养成分的变化主要集中在转色后期与完全转色期。PLS-DA结果表明不同转色时期的蓝靛果具有显著的差异,通过VIP>1共筛选出了16种对4个不同转色时期分化有显著贡献的营养组分。综上,蓝靛果的转色时期是其典型特征形成与功能性成分生物合成的关键阶段。
- Abstract:
- Taking blue honeysuckle at different colour change periods were taken as the test materials to investigate the changes in physicochemical properties and nutritional components during the colour change process.Techniques such as high-performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS) were used to detect the changing trends of physicochemical properties (hundred-fruit weight,soluble solids,titratable acid,pH),nutritional components (organic acids,saccharides,anthocyanins,polyphenols,flavonoids,minerals,amino acids),and antioxidant capacity (DPPH free radical scavenging capacity,ABTS free radical scavenging capacity,hydroxyl radical scavenging capacity) of Blue honeysuckle at four color-changing stages (uncolored stage,early color-changing stage,late color-changing stage,and fully colored stage).Meanwhile,partial least squares-discriminant analysis (PLS-DA) was performed.And a preliminary study on the correlation between physiological characteristics and the ripening of blue honeysuckle has been established,in order to provide a reference for the in-depth research of blue honeysuckle.The results showed that during the color change process,the hundred-fruit weight of blue honeysuckle showed no significant changing trend;soluble solids content,sugar content,and antioxidant capacity increased significantly;total acid and organic acids decreased significantly;mineral content generally showed a trend of first increasing and then decreasing;amino acid content showed a trend of first decreasing and then increasing.Meanwhile,the changes in these physicochemical indices and nutritional components were mainly concentrated in the late color-changing stage and fully colored stage.The PLS-DA results indicated that blue honeysuckle at different color-changing stages had significant differences.A total of 16 nutritional components that contributed significantly to the differentiation of the four color-changing stages were screened out based on VIP>1.These results indicated that the color-changing stage of Blue honeysuckle was a critical stage for the formation of its typical characteristics and the biosynthesis of functional components.
参考文献/References:
[1]王鑫,毕海鑫,修伟业,等.发酵蓝靛果果汁的工艺优化及香气成分分析[J].食品工业科技,2023,44(13):176-185.[2]SMOLIK M,OCHMIAN I,BOBROWSKA-CHWAT A,et al.Fingerprinting,structure,and genetic relationships among selected accessions of blue honeysuckle (Lonicera caerulea L.) from European collections[J].Biotechnology Reports,2022,34:e00721.[3]郑先哲,赵兴隆,刘成海,等.基于果实颜色特征的蓝靛果忍冬花青素含量预测[J].农业工程学报,2023,39(2):242-251.[4]ZHANG M,MA X M,XIAO Z,et al.Polyphenols in twenty cultivars of blue honeysuckle (Lonicera caerulea L.):Profiling,antioxidant capacity,and α-amylase inhibitory activity[J].Food Chemistry,2023,421:136148.[5]FAN L L,LIN L,ZHANG Y,et al.Component characteristics and reactive oxygen species scavenging activity of anthocyanins from fruits of Lonicera caerulea L.[J].Food Chemistry,2023,403:134391.[6]张龄予,潘丽佳,侯苏芯,等.蓝靛果多酚对免疫抑制小鼠调节肠道微生态的作用机制[J].食品科学,2023,44(15):147-155.[7]ZUO A,WANG S Q,LIU L,et al.Understanding the effect of anthocyanin extracted from Lonicera caerulea L.on alcoholic hepatosteatosis[J].Biomedicine & Pharmacotherapy,2019,117:109087.[8]AMARARATHNA M,HOSKIN D W,VASANTHA RUPASINGHE H P.Anthocyanin-rich haskap (Lonicera caerulea L.) berry extracts reduce nitrosamine-induced DNA damage in human normal lung epithelial cells in vitro[J].Food and Chemical Toxicology,2020,141:111404.[9]MENG X L,CAO H,LI H,et al.Effect of dietary honeysuckle (Lonicera caerulea L.) supplementation on lipid metabolism,immunity and intestinal microbiota in grass carp (Ctenopharyngodon idellus)[J].Aquaculture Reports,2022,23:101063.[10]WU S S,HE X,WU X S,et al.Inhibitory effects of blue honeysuckle (Lonicera caerulea L.) on adjuvant-induced arthritis in rats:Crosstalk of anti-inflammatory and antioxidant effects[J].Journal of Functional Foods,2015,17:514-523.[11]LIU S W,YU J C,GUO S,et al.Inhibition of pancreatic α-amylase by Lonicera caerulea berry polyphenols in vitro and their potential as hyperglycemic agents[J].LWT,2020,126:109288.[12]SHI J Y,XIAO Y H,JIA C L,et al.Physiological and biochemical changes during fruit maturation and ripening in highbush blueberry (Vaccinium corymbosum L.)[J].Food Chemistry,2023,410:135299.[13]MA B H,ZHAO X H,DAI B,et al.Changes in phytochemical compositions and antioxidant activity of Schisandra sphenathera fruit during ripening[J].Industrial Crops and Products,2023,199:116773.[14]LI Y,LI L,ZHANG X P,et al.Differences in total phenolics,antioxidant activity and metabolic characteristics in peach fruits at different stages of ripening[J].LWT,2023,178:114586.[15]AYAZ F A,DEMIR O,TORUN H,et al.Characterization of polyphenoloxidase (PPO) and total phenolic contents in medlar (Mespilus germanica L.) fruit during ripening and over ripening[J].Food Chemistry,2008,106(1):291-298.[16]GARRIDO I,URIARTE D,HERNNDEZ M,et al.The evolution of total phenolic compounds and antioxidant activities during ripening of grapes (Vitis vinifera L.,cv.tempranillo) grown in semiarid region:Effects of cluster thinning and water deficit[J].International Journal of Molecular Sciences,2016,17(11):1923.[17]LPEZ-HUERTAS E,PALMA J M.Changes in glutathione,ascorbate,and antioxidant enzymes during olive fruit ripening[J].Journal of Agricultural and Food Chemistry,2020,68(44):12221-12228.[18]AIRES A,CARVALHO R,MATOS M,et al.Variation of chemical constituents,antioxidant activity,and endogenous plant hormones throughout different ripening stages of highbush blueberry (Vaccinium corymbosumL.) cultivars produced in centre of Portugal[J].Journal of Food Biochemistry,2017,41(6):e12414.[19]VU H T,SCARLETT C J,VUONG Q V.Changes of phytochemicals and antioxidant capacity of banana peel during the ripening process;with and without ethylene treatment[J].Scientia Horticulturae,2019,253:255-262.[20]国家市场监督管理总局,国家卫生健康委员会.GB 12456-2021 食品安全国家标准 食品中总酸的测定(含第1号修改单)[S].北京:中国标准出版社,2021.[21]国家卫生健康委员会,国家市场监督管理总局.食品安全国家标准 食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖的测定:GB 5009.8—2023[S].北京:中国标准出版社,2023.[22]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准 食品中有机酸的测定:GB 5009.157—2016[S].北京:中国标准出版社,2017.[23]曾稳稳,李霄,丁昱文,等.不同市售蓝莓酒的营养成分分析[J].现代食品科技,2025,41(11):37-46.[24]国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准 食品中氨基酸的测定:GB 5009.124—2016[S].北京:中国标准出版社,2017.[25]孙欣彤,周文喜,陈阿音,等.荞麦叶茶半发酵工艺优化及其风味品质与抗氧化能力的综合评价[J/OL].食品工业科技,(2026-1-20)[2026-09-09].https://doi.org/10.13386/j.issn1002-0306.2026020051.[26]YAO H,JIN X,FENG M,et al.Evolution of volatile profile and aroma potential of table grape Hutai-8 during berry ripening[J].Food Research International,2021,143:110330.[27]YE Y H,ZHENG S Y,WANG Y X.Analysis of aroma components changes in Gannan navel orange at different growth stages by HS-SPME-GC-MS,OAV,and multivariate analysis[J].Food Research International,2024,175:113622.[28]MO X Q,CAI D,YANG H B,et al.Changes in fruit quality parameters and volatile compounds in four wampee varieties at different ripening stages[J].Food Chemistry:X,2025,27:102377.[29]WANG L X,JING T,CAI B Y,et al.Comparative analysis of physicochemical quality and metabolite profiles during fruit ripening in three banana cultivars using multivariate approaches[J].Postharvest Biology and Technology,2026,231:113847.[30]孙梓晶,韩树立,李敏敏,等.葡萄果实糖分合成机理研究进展[J].北方园艺,2025(24):115-122.[31]GUO L C,QIAO J L,ZHANG L J,et al.Critical review on anthocyanins in blue honeysuckle (Lonicera caerulea L.) and their function[J].Plant Physiology and Biochemistry,2023,204:108090.[32]樊勇,孙悦,录亿隆,等.观赏植物花青素合成调控机理及组学技术应用[J].江苏农业学报,2025,41(4):810-822.[33]胡佳诺,余梓萌,张心怡,等.莲的花色研究进展[J].植物科学学报,2023,41(6):800-808.[34]XU Y M,GE X Y,LV Y K,et al.Engineering plant hosts for high-efficiency accumulation of flavonoids:Advances,challenges and perspectives[J].Biotechnology Advances,2025,84:108692.
相似文献/References:
[1]刘艳霞,于长龙.蓝靛果酸奶的研制[J].北方园艺,2013,37(02):113.
LIU Yan-xia,YU Chang-long.The Development of Lonicera edulis Yogurt[J].Northern Horticulture,2013,37(17):113.
[2]程海涛,申健,肖志坚,等.伊春地区野生蓝靛果资源及群落特征的研究[J].北方园艺,2012,36(13):37.
CHENG Hai-tao,SHEN Jian,XIAO Zhi-jian,et al.Research on Resource and Community Characteristics of Wild Lonicera edulis in Yichun Region[J].Northern Horticulture,2012,36(17):37.
[3]张敏,刘刚,张雁南,等.响应面法优化蓝靛果花色苷提取工艺研究[J].北方园艺,2014,38(23):113.
ZHANG Min,LIU Gang,ZHANG Yan-nan,et al.Optimization of Extraction of Lonicera edulis Anthocyanin by Response Surface Methodology[J].Northern Horticulture,2014,38(17):113.
[4]刘 德 江,等.佳木斯地区野生蓝靛果引种及其栽培技术研究[J].北方园艺,2010,34(23):0.[doi:10.11937/bfyy.201023018]
LIU De -jiang,LIU Juan,et al.Study on Introduction Screening and Cultivation T echniques of Wild Lonicera edulis L. in Jiamusi Area[J].Northern Horticulture,2010,34(17):0.[doi:10.11937/bfyy.201023018]
[5]闫浩浩,米耀族,桑明玉,等.蓝靛果叶斑病病原高粱附球菌(Epicoccum sorghinum)分离鉴定及生物学特性[J].北方园艺,2023,(05):39.[doi:10.11937/bfyy.20222430]
(College of Resources and Environment,Northeast Agricultural University,Harbin,et al.YAN Haohao,MI Yaozu,SANG Mingyu,ZANG Hailian,LI Chunyan[J].Northern Horticulture,2023,(17):39.[doi:10.11937/bfyy.20222430]
备注/Memo
第一作者简介:解双瑜(1994-),女,博士研究生,助理研究员,现主要从事寒地果蔬加工等研究工作。E-mail:xiesy12345@163.com.责任作者:王志伟(1981-),男,硕士,副研究员,现主要从事寒地果蔬加工等研究工作。E-mail:542512358@qq.com.基金项目:国家重点研发计划资助项目(2022YFD1600505)。收稿日期:2025-11-26