DONG Bowen,LI Renping,LIU Yunlong,et al.Understory Cultivation of Different Herbal Plants on Soil Bacterial Community Structure[J].Northern Horticulture,2026,(9):99-109.[doi:10.11937/bfyy.20253896]
林下种植不同中草药对土壤细菌群落结构的影响
- Title:
- Understory Cultivation of Different Herbal Plants on Soil Bacterial Community Structure
- 文章编号:
- 1001-0009(2026)09-0099-11
- Keywords:
- herbal medicine; soil bacterial community; understory cultivation; high-throughput sequencing
- 分类号:
- S567
- 文献标志码:
- A
- 摘要:
- 以杨树林下裸地土壤,白薇、益母草、知母和射干林下土壤为试材，采集土壤样本并采用高通量测序的方法，对土壤细菌群落的多样性、丰富度等指标进行分析，研究了不同中草药在林下种植时对土壤细菌群落结构产生的影响，以期为林下中草药规范化种植提供参考依据。结果表明：不同中草药林下种植显著改变了土壤细菌群落结构，不同中草药处理下的土壤细菌群落组成存在明显差异，林下种植益母草时土壤的物种多样性和均匀度最高。不同处理下土壤细菌群落的优势菌门为变形菌门、酸杆菌门和放线菌门。种植益母草提高了芽单孢菌门的相对丰度。优势菌属为鞘氨醇单胞菌属和芽单胞菌属。鞘氨醇单胞菌属在射干下相对丰度占比最大，芽单胞菌属在种植知母土壤下相对丰度升高。根据LEfSe分析不同种类中草药土壤细菌结果显示，种植益母草土壤中有10个细菌类群明显偏高。进一步的PICRUSt2功能预测显示，林下种植益母草提高其碳水化合物代谢功能的相对丰度。综上，林下种植不同中草药有助于改善土壤细菌群落结构，优化土壤生态环境，且种植益母草时对改善土壤细菌群落和物种多样性效果更好。
- Abstract:
- Taking soil samples from bare land under poplar forest and under the cultivation of Cynanchum atratum,Leonurus japonicus,Anemarrhena asphodeloides，and Belamcanda chinensis as the test materials，high-throughput sequencing was conducted to analyze indicators such as diversity and richness of soil bacterial communities.The effects of understory cultivation of different herbal medicines on the soil bacterial community structure were investigated，in order to provide reference for the standardized understory cultivation practices of herbal medicines.The results showed that the cultivation of different herbal plants significantly altered the soil bacterial community structure，with clear differences in bacterial composition among treatments.The soil under Leonurus japonicus exhibited the highest species diversity and evenness.The dominant bacterial phyla across all treatments were Proteobacteria，Acidobacteria，and Actinobacteria.The cultivation of Leonurus japonicus increased the relative abundance of Gemmatimonadetes.The dominant genera were Sphingomonas and Gemmatimonas，with Sphingomonas being most abundant under Belamcanda chinensis and Gemmatimonas enriched under Anemarrhena asphodeloides.LEfSe analysis revealed 10 bacterial taxa significantly higher in Leonurus japonicus soil.PICRUSt2 functional prediction indicated that Leonurus japonicus cultivation enhanced the relative abundance of carbohydrate metabolism pathways.In conclusion，understory cultivation of different herbal plants can improve soil bacterial community structure and optimize soil ecological conditions，with Leonurus japonicus showing superior effects on enhancing soil bacterial diversity and community structure.
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备注/Memo
第一作者简介：董博文（1999-），女，硕士，助理工程师，现主要从事林下栽培等研究工作。E-mail：992947708@qq.com.责任作者：李仁平（1993-），男，硕士，工程师，现主要从事林下栽培等研究工作。E-mail:759702894@qq.com.基金项目：辽宁省农业科学院院长基金资助项目（2025QN2616）；中央财政林草科技推广示范资助项目（辽［2026］TG06号）。收稿日期：2025-11-12