[1]张缘秋,罗芹芬,赵继新,等.美洲南瓜白粉病抗性位点CpPM10.1近等基因系构建及抗病性分析[J].北方园艺,2026,(8):88-96.[doi:10.11937/bfyy.20253820]
　ZHANG Yuanqiu,LUO Qinfen,ZHAO Jixin,et al.Construction of the Near-isogenic Line and Disease Resistance Analysis of the Powdery Mildew Resistance Locus CpPM10.1 in Cucurbita pepo[J].Northern Horticulture,2026,(8):88-96.[doi:10.11937/bfyy.20253820]
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美洲南瓜白粉病抗性位点CpPM10.1近等基因系构建及抗病性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年8 页码: 88-96 栏目: 出版日期: 2026-04-30
Title:
Construction of the Near-isogenic Line and Disease Resistance Analysis of the Powdery Mildew Resistance Locus CpPM10.1 in Cucurbita pepo
文章编号:
1001-0009(2026)08-0088-09
作者:
张缘秋1; 2; 罗芹芬1; 2; 赵继新1; 2; 马佳欣1; 2; 屈淑平1; 2; 王云莉1; 2
（1.东北农业大学 园艺园林学院，黑龙江 哈尔滨 150030；2.东北农业大学 农业农村部东北地区园艺作物生物学与遗传改良重点实验室，黑龙江 哈尔滨 150030）
Author(s):
ZHANG Yuanqiu1; 2; LUO Qinfen1; 2; ZHAO Jixin1; 2; MA Jiaxin1; 2; QU Shuping1; 2; WANG Yunli1; 2
(1.College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin,Heilongjiang 150030;2.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region)，Ministry of Agriculture and Rural Affairs,Northeast Agricultural University，Harbin,Heilongjiang 150030)
关键词:
美洲南瓜; 近等基因系; 白粉病抗性; 抗病性分析
Keywords:
Cucurbita pepo; near-isogenic line; powdery mildew resistance; disease resistance analysis
分类号:
S642.1
DOI:
10.11937/bfyy.20253820
文献标志码:
A
摘要:
以美洲南瓜白粉病高抗自交系‘X10’为供体亲本试材，以高感自交系‘JIN234’为轮回亲本试材，采用前景和背景选择结合多代回交的方法构建了白粉病抗性位点CpPM10.1近等基因系‘NIL-PM10.1’，研究了位点CpPM10.1对白粉病抗性的影响，以期为南瓜白粉病抗性育种提供参考依据。结果表明：以CpPM10.1紧密连锁分子标记为前景选择标记，以210个多态性好、在美洲南瓜20条染色体均匀分布的分子标记为背景选择标记，检测到抗性位点CpPM10.1已导入近等基因系‘NIL-PM10.1’中，且‘NIL-PM10.1’的背景恢复率达99.52%。接种白粉菌后，‘NIL-PM10.1’叶片分生孢子的萌发时间比‘JIN234’延迟了，且孢子产生少，菌丝伸长扩增速度明显减缓；接菌7 d后‘NIL-PM10.1’的病情指数显著低于‘JIN234’。在侵染关键时期，‘NIL-PM10.1’的CAT、SOD、POD活性显著高于‘JIN234’，H2O2含量始终低于‘JIN234’。
Abstract:
The Cucurbita pepo of powdery mildew (PM) high-resistance inbred line ‘X10’ was used as the donor parent material，and the Cucurbita pepo of PM high-susceptibility inbred line ‘JIN234’ was used as the recurrent parent material.The near-isogenic line of PM resistance locus CpPM10.1，‘NIL-PM10.1’，was constructed by using the method of foreground and background selection combined with multi-generation backcrossing.The influence of locus CpPM10.1 on PM resistance was studied，in order to provide reference for PM resistance breeding in Cucurbita pepo.The results showed that markers closely linked to CpPM10.1 locus as the foreground selection markers and 210 markers with good polymorphism and uniform distribution on 20 chromosomes as the background selection markers，and resistance locus CpPM10.1 was detected to be transferred into the near-isogenic line ‘NIL-PM10.1’ with a background recovery rate of 99.52%.Compared with ‘JIN234’，the conidia germination on the leaves of ‘NIL-PM10.1’ was delayed after PM inoculation，spore production of ‘NIL-PM10.1’ was few，and the growth and expansion speed of hyphal in ‘NIL-PM10.1’ were significantly slowed down.Moreover，the disease index of ‘NIL-PM10.1’ was significantly lower than that of ‘JIN234’ at 7 days after PM inoculation.During the critical period of PM infection，the CAT，SOD and POD activities of ‘NIL-PM10.1’ were significantly higher than those of ‘JIN234’，and the H2O2 content of ‘NIL-PM10.1’ was significantly lower than that of ‘JIN234’.

参考文献/References:

［1］VOEGELE R T,STRUCK C,HAHN M,et al.The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae［J］.PNAS,2001,98(14):8133-8138.［2］STAPLES R C.Nutrients for a rust fungus:The role of haustoria［J］.Trends in Plant Science,2001,6(11):496-498.［3］杨美娟,黄坤艳,韩庆典.小麦白粉病及其抗性研究进展［J］.分子植物育种,2016,14(5):1244-1254.［4］李艳,高宝嘉,陈连涛.植物诱导抗性的分子机制研究进展［J］.河北林果研究,2013(4):427-430.［5］GAO X,GUO P,WANG Z,et al.Transcriptome profiling reveals response genes for downy mildew resistance in cucumber［J］.Planta,2021,253(5):112.［6］田丽波,商桑,杨衍,等.苦瓜对白粉病的抗性与相关生理生化指标的关系［J］.西北农业学报,2015,24(9):166-173.［7］陈夕军,朱键鑫,陈羽,等.抗白粉病黄瓜品种的叶片组织结构及其生理生化［J］.江苏农业学报,2015,31(1):55-61.［8］张兆辉,卢盼玲,陈春宏,等.西葫芦抗白粉病的生理生化机制［J］.分子植物育种,2021,19(9):3074-3080.［9］WANG Y,QI C,LUO Y,et al.Identification and mapping of CpPM10.1,a major gene involved in powdery mildew (race 2 France of Podosphaera xanthii) resistance in zucchini (Cucurbita pepo L.)［J］.Theoretical and Applied Genetics,2021,134(8):2531-2545.［10］GUO W L,CHEN B H,CHEN X J,et al.Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew［J］.PLoS One,2018,13(1):e0190175.［11］GUO W L,CHEN B H,GUO Y Y,et al.Improved powdery mildew resistance of transgenic Nicotiana benthamiana overexpressing the Cucurbita moschata CmSGT1 gene［J］.Frontiers in Plant Science,2019,10:955.［12］GUO W L,CHEN B H,GUO Y Y,et al.Corrigendum:Expression of pumpkin CmbHLH87 gene improves powdery mildew resistance in tobacco［J］.Frontiers in Plant Science,2021,12:779320.［13］GUO W L,HE S T,GUO Y Y,et al.A pumpkin MYBR1 transcription factor,CmMYB1,increased susceptibility to biotic stresses in transgenic tobacco［J］.Russian Journal of Plant Physiology,2022,69(6):134.［14］GUO W L,YANG H L,ZHAO J P,et al.A pathogenesis-related protein 1 of Cucurbita moschata responds to powdery mildew infection［J］.Frontiers in Genetics,2023,14:1168138.［15］LI S H,WANG P.Overexpression of pumpkin CpVQ30 increased susceptibility of tobacco to powdery mildew［J］.Physiological and Molecular Plant Pathology,2024,133:102365.［16］张丰.美洲南瓜白粉病抗性位点CpPM10.1基因分析及近等基因系构建［D］.哈尔滨:东北农业大学,2022.［17］NIE J,WANG Y,HE H,et al.Loss-of-function mutations in CsMLO1 confer durable powdery mildew resistance in cucumber (Cucumis sativus L.)［J］.Frontiers in Plant Science,2015,6:1155.［18］HAN J,DONG S,LIU X,et al.Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber［J］.Frontiers in Plant Science,2022,13:968811.［19］方钰.黄瓜-酸黄瓜渐渗系IL52抗霜霉病主效QTL dm5.1及园艺性状的遗传定位［D］.南京:南京农业大学,2023.［20］杨侃侃,刘晓虹,陈惠明,等.黄瓜枯萎病抗性的遗传分析［J］.中国瓜菜,2024,37(10):47-55.［21］TORRES M A,JONES J D G,DANGL J L.Reactive oxygen species signaling in response to pathogens［J］.Plant Physiology,2006,141(2):373-378.［22］DONG W,ZHANG Y,WANG Y,et al.Comparative proteomic and metabolomic analysis of resistant and susceptible Kentucky Bluegrass cultivars in response to infection by powdery mildew［J］.BMC Plant Biology,2024,24(1):1195.［23］冯丽丹,李捷,何静,等.喷施BABA调控枸杞活性氧代谢提高果实采后抗病能力［J］.北方园艺,2023(13):112-120.［24］李润雨,蒋帆,张佳婧,等.柿种质资源炭疽病抗性评价及生理指标相关性分析［J/OL］.北方园艺,(2026-01-28)［2026-01-29］.https://link.cnki.net/urlid/23.1247.S.20260106.1036.020.［25］余长洪,黄秋月,陈扬,等.贝莱斯芽孢杆菌N13生物菌肥对辣椒根腐病的防治及抗性酶活性的影响［J/OL］.北方园艺,(2026-01-28)［2026-01-29］.https://link.cnki.net/urlid/23.1247.S.20251215.1705.002.

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备注/Memo

第一作者简介：张缘秋(2000-),女,硕士研究生,研究方向为南瓜分子育种。E-mail:2635171056@qq.com.责任作者：王云莉 (1985-),女,博士,副教授,现主要从事南瓜分子育种等研究工作。E-mail:wangyunli@neau.edu.cn.基金项目：黑龙江省重点研发计划资助项目(GA23B007)；国家自然青年科学基金资助项目(32002051)。收稿日期：2025-11-05

更新日期/Last Update: 2026-05-06