LIU Zhe,WANG Chaojie,CHENG Yaqian,et al.Optimization and Establishment of Regeneration System of Cucurbita spp.[J].Northern Horticulture,2026,(6):1-9.[doi:10.11937/bfyy.20253636]
南瓜再生体系的优化与建立
- Title:
- Optimization and Establishment of Regeneration System of Cucurbita spp.
- 文章编号:
- 1001-0009(2026)06-0001-09
- Keywords:
- Cucurbita spp.; regeneration system; genotype; phytohormone
- 分类号:
- S642.1
- 文献标志码:
- A
- 摘要:
- 以不同基因型南瓜为试材，采用组织培养技术，研究了外植体类型、基因型和不同激素配比对不定芽诱导、伸长和生根等的影响，以期建立稳定高效的南瓜再生体系并为南瓜遗传转化提供参考依据。结果表明：不同类型外植体不定芽的诱导率存在显著差异，1/2子叶节不定芽诱导率均显著高于1/4子叶节；不同基因型南瓜再生效率差异显著，利用再生率较高的美洲南瓜材料“19”、中国南瓜材料“中国南瓜2号”和印度南瓜材料“282”进行最适激素配比试验，“19”不定芽诱导的最优激素组合为MS+6-BA 2.0 mg·L-1+ABA 0.5 mg·L-1，不定芽诱导率为88.3%；“中国南瓜2号”不定芽诱导的最优激素组合为MS+6-BA 2.0 mg·L-1+ABA 1.0 mg·L-1，不定芽诱导率为85.0%；“282”不定芽诱导的最优激素组合为MS+6-BA 1.0 mg·L-1+ABA 1.0 mg·L-1，不定芽诱导率为100.0%；南瓜不定芽伸长的最适激素配比为MS+KT 0.2 mg·L-1，不定芽长势好且粗壮；不定芽在不添加激素的MS培养基中生根效果最好，根长且发达。
- Abstract:
- Taking different genotypes of Cucurbita spp.as the experimental materials,tissue culture techniques was used to investigate the effects of explant types，genotypes，and varying hormone combinations on the induction，elongation，and rooting of adventitious buds，in order to establish a stable and highly efficient regeneration system for Cucurbita spp.，thereby providing a reference for genetic transformation studies in this species.The results showed that there were significant differences in the induction rates of adventitious buds from different types of explants.The induction rate of adventitious buds from 1/2 leaf nodes was significantly higher than that from 1/4 leaf nodes.The regeneration efficiency with different genotypes was significantly different.C.pepo inbred line ‘19’，C.moschata inbred line ‘C.moschata No.2’ and C.maxima inbred line ‘282’ with high regeneration efficiency were selected to carry on the subsequent optimal hormone ratio test.The combination of MS+6-BA 2.0 mg·L-1+ABA 0.5 mg·L-1 was the optimal medium for bud induction with a 88.3% induction rate of ‘19’.The combination of MS+6-BA 2.0 mg·L-1+ABA 1.0 mg·L-1 was the optimal medium for bud induction with a 85.0% induction rate of ‘C.moschata No.2’.The combination of MS+6-BA 1.0 mg·L-1+ABA 1.0 mg·L-1 was the optimal medium for bud induction with a 100.0% induction rate of ‘282’.MS+KT 0.2 mg·L-1 was the optimal medium for adventitious bud elongation with strong shoots and growth in the three genotypes Cucurbita spp.MS without added hormones was the optimal medium for shoot rooting with the long and developed roots.
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备注/Memo
第一作者简介：刘喆（1998-），女，硕士，研究实习员，现主要从事大豆遗传育种等研究工作。E-mail:18104602239@163.com.责任作者：屈淑平（1973-），女，博士，教授，现主要从事南瓜遗传育种等研究工作。E-mail：spqu@neau.edu.cn.基金项目：黑龙江省自然科学基金重点资助项目（ZD2024C001）；国家自然科学基金资助项目（32272723）。收稿日期：2025-10-20