[1]潘佳圆,李伟佳,赵建国,等.月季花FRIGIDA基因家族的全基因组鉴定及生物信息学分析[J].北方园艺,2026,(8):1-10.[doi:10.11937/bfyy.20253540]
　PAN Jiayuan,LI Weijia,ZHAO Jianguo,et al.Genome-wide Identification and Bioinformatics Analysis of the FRIGIDA Gene Family in Rosa chinensis[J].Northern Horticulture,2026,(8):1-10.[doi:10.11937/bfyy.20253540]
点击复制

月季花FRIGIDA基因家族的全基因组鉴定及生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年8 页码: 1-10 栏目: 出版日期: 2026-04-30
Title:
Genome-wide Identification and Bioinformatics Analysis of the FRIGIDA Gene Family in Rosa chinensis
文章编号:
1001-0009(2026)08-0001-10
作者:
潘佳圆1; 2; 李伟佳1; 2; 赵建国1; 2; 乔俊1; 2
（1.山西大同大学 煤基生态碳汇技术教育部工程研究中心，山西 大同 037005；2.山西大同大学 石墨烯林业应用国家林草局重点实验室，山西 大同 037005）
Author(s):
PAN Jiayuan1; 2; LI Weijia1; 2; ZHAO Jianguo1; 2; QIAO Jun1; 2
(1.Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education，Shanxi Datong University，Datong，Shanxi 037005;2.Key Laboratory of Graphene Forestry Application of National Forest and Grass Administration，Shanxi Datong University，Datong，Shanxi 037005)
关键词:
月季花; FRI; 基因家族; 开花
Keywords:
Rosa chinensis; FRI; gene family; flowering
分类号:
S685.12
DOI:
10.11937/bfyy.20253540
文献标志码:
A
摘要:
以月季花为试材，采用全基因组鉴定结合染色体定位、系统进化树构建、共线性分析及启动子顺式元件预测等生物信息学方法，研究了RcFRI基因家族的分子特性及其进化机制，以期为揭示该家族在月季花生长发育及逆境适应中的功能分化提供参考依据。结果表明：月季花共鉴定出21个RcFRI成员，分布于7条染色体；其编码蛋白分子量为54~111 kDa，其中18个定位于细胞核，3个呈核质双定位。系统进化树分析表明，该家族与水稻、拟南芥同源基因共同分为5个亚组；组Ⅴ（含16个RcFRI）呈现月季花特异性富集，提示其参与物种特化性状调控。基因结构分析证实所有成员均含UTR和CDS结构域，保守基序motif 2/3分布最广泛，保守结构域属于FRIGIDA超家族。启动子区检测到大量光响应元件（Light）、脱落酸响应元件（ABA）及激素相关元件，说明该家族可能介导光信号转导与逆境胁迫响应。共线性分析显示家族内存在7次复制事件，而与拟南芥仅存在3对同源基因，表明二者进化亲缘关系较远。
Abstract:
Taking Rosa chinensis as the experimental material，bioinformatics methods included genome-wide identification combined with chromosomal localization，phylogenetic tree construction，synteny analysis，and promoter cis-acting element prediction were carried out to investigate the molecular characteristics and evolutionary mechanism of the RcFRI gene family，in order to provide reference for revealing the functional differentiation of this family in the growth，development and stress adaptation of Rosa chinensis.The results showed that 21 RcFRI members were identified distributed across 7 chromosomes in Rosa chinensis.The molecular weights of the proteins they encoded ranged from 54 to 111 kDa.18 RcFRI were localized in the nucleus.3 RcFRI showed nucleocytoplasmic dual localization.Phylogenetic tree analysis indicated that this family，along with homologous genes from Oryza sativa and Arabidopsis thaliana，was categorized into 5 subgroups.Subgroup V，which contained 16 RcFRI members，had Rosa chinensis enrichment，suggesting its participation in regulating species traits.Gene structure analysis verified that all members had UTR and CDS domains.Conserved motifs 2/3 were the most extensively distributed.The conserved domain belonged to the FRIGIDA superfamily.In the promoter region，a substantial number of light elements (Light)，abscisic acid elements (ABA)，and hormone elements were detected，meaning this family might mediate light signal transduction and stress responses.Synteny analysis disclosed 7 duplication events within the family.Only 3 pairs of homologous genes were recognized with Arabidopsis thaliana，showing a relatively remote evolutionary relationship.

参考文献/References:

［1］张佐双,许桂花.花中的皇后:月季［J］.园林,2007,24(10):14-15.［2］黄承丽.月季花卉的发展研究探讨［J］.现代园艺,2013,36(20):24-25.［3］YI X,GAO H,YANG Y,et al.Differentially expressed genes related to flowering transition between once-and continuous-flowering roses［J］.Biomolecules,2021,12(1):58.［4］YU C,GUO X,LUO L,et al.Species-specific genes account for the differences in floral transition between continuous-flowering and once-flowering roses［J］.Journal of Plant Biochemistry and Biotechnology,2019,28(3):312-319.［5］ZHANG Z,YANG T,LIU Y,et al.Haplotype-resolved genome assembly and resequencing provide insights into the origin and breeding of modern rose［J］.Nature Plants,2024,10(11):1659-1671.［6］中国花卉协会月季分会.中国月季发展报告(第2版)［J］.农业科技与信息(现代园林),2014,11(5):1-47.［7］SHINDO C,ARANZANA M J,LISTER C,et al.Role of FRIGIDA and FLOWERING LOCUS C in determining variation in flowering time of Arabidopsis［J］.Plant Physiology,2005,138(2):1163-1173.［8］JOHANSON U,WEST J,LISTER C,et al.Molecular analysis of FRIGIDA,a major determinant of natural variation in Arabidopsis Flowering time［J］.Science,2000,290(5490):344-347.［9］GERALDO N,BURLE I,KIDOU S I,et al.FRIGIDA delays flowering in Arabidopsis via a cotranscriptional mechanism involving direct interaction with the nuclear cap-binding complex［J］.Plant Physiology,2009,150(3):1611-1618.［10］胡思凡.枳PtFRI基因在成花过程中的功能分析［D］.武汉:华中农业大学,2018.［11］闫笑.百合春化和光周期开花途径关键基因的筛选及作用机制分析［D］.北京:北京林业大学,2022.［12］邱美琪.柑橘CiFRI基因在干旱胁迫中的作用［D］.武汉:华中农业大学,2021.［13］翟江园.蜡梅开花相关的FRIGIDA-LIKE基因家族成员功能的初步探究［D］.武汉:华中农业大学,2020.［14］易丽聪.甘蓝型油菜开花调控因子BnaA3.FRI的功能分析和基于染色体代换系的花期QTL定位［D］.武汉:华中农业大学,2018.［15］MAQSOOD K.SlFRIGIDA-LIKE和SlBIN2及其互作调控番茄生长、株型、产量及冷胁迫反应［D］.杨凌:西北农林科技大学,2024.［16］YU S,WANG Y,REN W,et al.Comprehensive genome-wide analysis of the GmFRIGIDA gene family in soybean:Identification,characterization,and expression dynamics［J］.Frontiers in Plant Science,2025,16:1536866.［17］POTTER S C,LUCIANI A,EDDY S R,et al.HMMER web server:2018 update［J］.Nucleic Acids Research,2018,46(W1):W200-W204.［18］EDGAR R C.MUSCLE:Multiple sequence alignment with high accuracy and high throughput［J］.Nucleic Acids Research,2004,32(5):1792-1797.［19］沈琦,张春楠,丁美云,等.芍药三种蔗糖代谢关键基因家族鉴定及生物信息学分析［J］.北方园艺,2025(19):8-14.［20］LESCOT M,DHAIS P,THIJS G,et al.PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences［J］.Nucleic Acids Research,2002,30(1):325-327.［21］THUMULURI V,ALMAGRO ARMENTEROS J J,JOHANSEN A R,et al.DeepLoc 2.0:Multi-label subcellular localization prediction using protein language models［J］.Nucleic Acids Research,2022,50(W1):W228-W234.［22］EL-GEBALI S,MISTRY J,BATEMAN A,et al.The Pfam protein families database in 2019［J］.Nucleic Acids Research,2019,47(D1):D427-D432.［23］LETUNIC I,BORK P.Interactive Tree Of Life (iTOL) v5:An online tool for phylogenetic tree display and annotation［J］.Nucleic Acids Research,2021,49(W1):W293-W296.［24］WANG Y,TANG H,DEBARRY J D,et al.MCScanX:A toolkit for detection and evolutionary analysis of gene synteny and collinearity［J］.Nucleic Acids Research,2012,40(7):e49.［25］孙姝琦,邹志勇,刘晓涵,等.冰菜WRKY基因家族的全基因组鉴定、功能特征及表达模式分析［J］.北方园艺,2025(20):31-41.［26］CHEN S,ZHOU Y,CHEN Y,et al.Fastp:An ultra-fast all-in-one FASTQ preprocessor［J］.Bioinformatics,2018,34(17):i884-i890.［27］CHEN C,WU Y,LI J,et al.TBtools-Ⅱ:A “one for all,all for one” bioinformatics platform for biological big-data mining［J］.Molecular Plant,2023,16(11):1733-1742.［28］LI H,GAO J,SHI T,et al.Genome-wide identification and expression analysis of the frigida domain gene family in Prunus mume (Prunus mume Sieb.et Zucc.)［J］.Horticulture,Environment,and Biotechnology,2021,62(5):817-828.［29］CHOI K,KIM J,HWANG H J,et al.The FRIGIDA complex activates transcription of FLC,a strong flowering repressor in Arabidopsis,by recruiting chromatin modification factors［J］.The Plant Cell,2011,23(1):289-303.［30］TOYOTA M,MATSUDA K,KAKUTANI T,et al.Developmental changes in crossover frequency in Arabidopsis［J］.The Plant Journal,2011,65(4):589-599.［31］HIBRAND SAINT-OYANT L,RUTTINK T,HAMAMA L,et al.A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits［J］.Nature Plants,2018,4(7):473-484.

备注/Memo

第一作者简介：潘佳圆（1997-），女，硕士研究生，研究方向为碳纳米材料的生物效应。E-mail:1214798755@qq.com.责任作者：乔俊（1983-），男，博士，副教授，现主要从事碳纳米材料在环境中的生物效应、污染环境的生物修复等研究工作。E-mail:qiaojun_nk@163.com.基金项目：山西省科技厅基础研究计划资助项目（202303021221171）。收稿日期：2025-10-11

更新日期/Last Update: 2026-05-06