QUAN Yan,ZHANG Liyan,ZHAO Jun,et al.Identification and Biological Characteristics of Pathogens Causing Damping-off Disease in Introduced Cherry Seedlings[J].Northern Horticulture,2026,(7):92-98.[doi:10.11937/bfyy.20253539]
引种樱桃猝倒病病原菌鉴定及生物学特性研究
- Title:
- Identification and Biological Characteristics of Pathogens Causing Damping-off Disease in Introduced Cherry Seedlings
- 文章编号:
- 1001-0009(2026)07-0092-07
- Keywords:
- cherry damping-off; Alternaria tenuissima; biological characteristics; new host record; arid area; seedling introduction
- 分类号:
- S436.412
- 文献标志码:
- A
- 摘要:
- 以引种樱桃苗木为试材，采用病原菌分离鉴定、致病力测定、生物学特性分析及营养亲和群结构分析等综合方法，研究了细极链格孢菌对引种樱桃苗木猝倒病的致病作用，以期为干旱区樱桃引种栽培中苗木猝倒病的防控及资源高效利用提供参考依据。结果表明：经形态学与分子生物学联合鉴定，明确引起樱桃猝倒病的病原菌为细极链格孢菌（Alternaria tenuissima），该研究为该病原在樱桃上的首次报道；致病力测定结果证实该病原菌具有致病性；生物学特性分析显示，该菌最适生长温度为25 ℃、最适pH为6.0；营养亲和群结构分析筛选出优势菌群G1及At-c-1、At-c-2、At-c-3等13个优势菌株。
- Abstract:
- Taking introduced cherry seedlings as test materials,this study adopted comprehensive methods including pathogen isolation and identification,pathogenicity determination,biological characteristic analysis and vegetative compatibility group (VCG) structure analysis to investigate the pathogenic effect of Alternaria tenuissima on the damping-off disease of introduced cherry seedlings,in order to provide a reference for the prevention and control of seedling damping-off disease and the efficient utilization of resources in the introduction and cultivation of cherries in arid areas.The results showed that,through the combined identification of morphology and molecular biology,the pathogen causing cherry damping-off disease was identified as Alternaria tenuissima,which was the first report of this pathogen on cherries in this study; the results of pathogenicity determination confirmed that this pathogen was pathogenic; the analysis of biological characteristics indicated that the optimal growth temperature of this fungus was 25 ℃ and the optimal pH was 6.0; the analysis of vegetative compatibility group structure screened out the dominant group G1 and 13 dominant strains such as At-c-1,At-c-2 and At-c-3.
参考文献/References:
［1］刘子夜,武琳霞,刘美玉,等.山东地区不同品种樱桃营养品质评价［J］.食品安全质量检测学报,2025,16(2):35-45.［2］朱弘.尾叶樱桃(Cerasus dielsiana)系统分类地位与种群生物地理学研究［D］.南京:南京林业大学,2020.［3］陈玲玲,张书东,邰思涵,等.‘红阳’猕猴桃软腐病病原菌细极链格孢菌的基因组组装与功能分析［J］.六盘水师范学院学报,2024,36(3):92-100.［4］刘延锋,曾洪梅,玉山江,等.极细链格孢菌〖STBX〗peaT1〖STBZ〗基因在毕赤酵母中的表达与功能分析［J］.生物工程学报,2009,25(3):413-417.［5］王洪凯,张天宇,张猛.链格孢属真菌分类研究进展［J］.山东农业大学学报(自然科学版),2001,32(3):406-410.［6］刘丰银.禾本科植物链格孢菌多样性研究［D］.荆州:长江大学,2024.［7］宋福,赵文胜,严进,等.鸭梨包装环节和出口前检验对链格孢菌侵染的影响［J］.植物保护,2006,32(2):84-86.［8］李根.苗木猝倒病防控对策［J］.农民致富之友,2012(9):86.［9］方中达.植病研究方法［M］.3版.北京:中国农业出版社,1998.［10］孟韫思,胡亚龙,王畅如,等.龙牙百合鳞茎腐烂病病原鉴定、生物学特性及杀菌剂的筛选［J］.北方园艺,2026(3):85-92.［11］杨远桥.贵州省果树主要真菌性病害真菌鉴定及系统学分析［D］.贵阳:贵州大学,2023.［12］石晓燕,石洁,李前进,等.苹果水烂病病原菌鉴定及防治药剂筛选［J］.北方园艺,2025(2):67-75.［13］谢林,蔡海林,李建勇,等.长沙烟区烟草赤星病病害调查与病原菌鉴定［J］.中国烟草科学,2025,46(2):45-51.［14］杜亚敏.引起猕猴桃枝枯和叶斑的间座壳菌种类鉴定及致病性研究［D］.武汉:华中农业大学,2020.［15］李洋,赵飞,邢鲲,等.甜瓜蔓枯病生物学特性及防治药剂筛选［J］.中国果菜,2024,44(12):84-89.［16］徐莹,李青峰,张睿辰,等.平贝母菌核病病原鉴定及其生物学特性分析［J］.北方园艺,2025(1):97-104.［17］常媛,杨兴堂,姜传英,等.一株能拮抗3种土传病害病原真菌的长枝木霉［J］.草业科学,2017,34(2):246-254.［18］张福兴,董向丽,潘凤荣,等.甜樱桃流胶病研究综述［J］.烟台果树,2015(3):1-5.［19］田亚晨,贡汉生,赵珊,等.烟台地区引起甜樱桃采后腐烂霉菌的分离鉴定［J］.食品科学,2017,38(22):28-33.［20］尚晓静,罗妮星,张富美,等.树莓极细链格孢菌叶斑病病原菌鉴定及其生物学特性［J］.北方园艺,2021(16):24-32.［21］冯飞,谢振文,曾慕衡.一种蛋白激发子产生菌细极链格孢菌的生物学特性研究［J］.安徽农业科学,2009,37(9):3960-3961,4056.［22］秦小杰.芍药响应细极链格孢侵染的转录组与代谢组学分析及抗病基因挖掘［D］.泰安:山东农业大学,2023.［23］鲁中华.外源茉莉酸甲酯对芍药响应细极链格孢菌侵染的影响及抗病机理研究［D］.泰安:山东农业大学,2024.［24］王亚娟.采后蓝莓果实响应细极链格孢菌侵染的抗病相关基因挖掘及调控机理研究［D］.沈阳:沈阳农业大学,2023.［25］张建强,ABDRAMANE S Z,王江来,等.芹菜叶斑病病原菌细极链格孢药剂筛选［J］.新疆农业科学,2022,59(7):1748-1757.［26］MAJIDI M,MIRJALILI M H,FARZANEH M,et al.Fungal endophytes Fusarium solani SGGF14 and Alternaria tenuissima SGGF21 enhance the glycyrrhizin production by modulating its key biosynthetic genes in licorice (Glycyrrhiza glabra L.)［J］.Journal of Applied Microbiology,2024,135(8):lxae199.［27］SINGH K,KUMARI P,GUPTA R K,et al.First report of Alternaria tenuissima causing leaf black spot on Aloe barbadensis in India［J］.Journal of Plant Pathology,2024,106(3):1395-1396.［28］SINGH K,DEEPA N,CHAUHAN S,et al.Antifungal action of 1,8 cineole,a major component of Eucalyptus globulus essential oil against Alternaria tenuissima via overproduction of reactive oxygen species and downregulation of virulence and ergosterol biosynthetic genes［J］.Industrial Crops and Products,2024,214:118580.［29］JI S,LIU B,HAN J,et al.Bacillus-derived consortium enhances Ginkgo biloba′s health and resistance to Alternaria tenuissima［J］.Pest Management Science,2024,80(8):4110-4124.
备注/Memo
第一作者简介：全艳（2002-），女，硕士研究生，研究方向为森林保护。E-mail:2535677905@qq.com.责任作者：张丽艳（1983-），女，博士，副教授，现主要从事真菌病毒、植物分子免疫、植物病原物致病机理及抗逆基因分子设计育种等研究工作。E-mail:zhangliyanyong@126.com.基金项目：内蒙古自治区自然科学基金资助项目（2023LHMS03007）；内蒙古农业大学高层次/优秀博士人才引进科研启动资助项目（NDYB2021-6）。收稿日期：2025-10-11