TIAN Heting,ZONG Zixuan,FENG Xinyi,et al.Analysis of Exogenous Sulfur Alleviating Cadmium Toxicity in Chrysanthemum indicum[J].Northern Horticulture,2026,(8):65-72.[doi:10.11937/bfyy.20253538]
外源硫缓解野菊镉毒害分析
- Title:
- Analysis of Exogenous Sulfur Alleviating Cadmium Toxicity in Chrysanthemum indicum
- 文章编号:
- 1001-0009(2026)08-0065-08
- Keywords:
- exogenous sulfur; Chrysanthemum indicum; cadmium poisoning
- 分类号:
- S682.1+1
- 文献标志码:
- A
- 摘要:
- 以高、中、低3种耐镉性野菊(Chrysanthemum indicum)为试材，采用液培法，研究了不同浓度（2.70、1 502.70、6 502.70 μmol·L-1）的外源硫对镉胁迫处理下野菊生长生理、吸收和转运镉、抗氧化酶活性、抗氧化物质及植物螯合肽含量的影响，以期为植物镉污染修复提供参考依据。结果表明：1）外源硫缓解镉对野菊根茎伸长及生物量积累的抑制作用；2）外源过量硫减少野菊根系对镉的吸收并增强镉向地上部分的转运能力；3）外源硫增强多种抗氧化酶（SOD、CAT、APX、GR）活性，减轻氧化胁迫；4）外源硫促进多种非酶类抗氧化物质（AsA、GSH、NPT）及植物螯合肽（PCs）含量的增加，参与ROS的清除并增强对镉的螯合作用，减少细胞中游离态镉的含量。综上可知，外源硫可以通过减少野菊根系对镉的吸收，增强镉转运能力，启动抗氧化系统防御机制并增强螯合作用，维持野菊生长稳定来缓解野菊镉毒害。
- Abstract:
- Taking Chrysanthemum indicum with high，medium，and low cadmium tolerance as experimental materials，hydroponic culture was employed to investigate the physiological growth，cadmium uptake and transport，antioxidant enzyme activity，and antioxidant substance levels of wild Chrysanthemum under cadmium stress treatment supplemented with exogenous sulfur at different concentrations (2.70，1 502.70，6 502.70 μmol·L-1) on the physiological growth，cadmium uptake and transport，antioxidant enzyme activity，antioxidant content，and plant chelating peptide levels under cadmium stress,in order to provide a reference for plant-based cadmium pollution remediation.The results showed that,1) exogenous sulfur alleviated the inhibition of cadmium on rhizome elongation and biomass accumulation in Chrysanthemum indicum.2) Excessive exogenous sulfur reduced cadmium uptake by roots and enhanced cadmium transport to the aboveground parts.3) Exogenous sulfur increased the activities of multiple antioxidant enzymes (SOD，CAT，APX，GR) and alleviated oxidative stress.4) Exogenous sulfur promotes increases in various non-enzymatic antioxidants (AsA，GSH，NPT) and plant chelating peptides (PCs)，participates in ROS scavenging，enhances cadmium chelation，and reduces free cadmium content in cells.In summary，exogenous sulfur alleviates cadmium toxicity in Chrysanthemum indicum by reducing cadmium uptake in roots，enhancing cadmium transport capacity，activating antioxidant defense mechanisms，and strengthening chelation effects，thereby maintaining stable plant growth.
参考文献/References:
［1］SHAO X,YAO H,CUI S,et al.Activated low-grade phosphate rocks for simultaneously reducing the phosphorus loss and cadmium uptake by rice in paddy soil［J］.Science of the Total Environment,2021,780:146550.［2］PARKER G H，GILLIE C E，MILLER J V，et al.Human health risk assessment of arsenic，cadmium，lead，and mercury ingestion from baby foods［J］.Toxicology Reports，2022（9）：238-249.［3］ROMERO-PUERTAS M C,TERRN-CAMERO L C,PELEZ-VICO M ,et al.Reactive oxygen and nitrogen species as key indicators of plant responses to Cd stress［J］.Environmental and Experimental Botany,2019,161:107-119.［4］FARMER E E,MUELLER M J.ROS-mediated lipid peroxidation and RES-activated signaling［J］.Annual Review of Plant Biology,2013,64:429-450.［5］KHAN M I R,IQBAL N,MASOOD A,et al.Modulation and significance of nitrogen and sulfur metabolism in cadmium challenged plants［J］.Plant Growth Regulation,2016,78(1):1-11.［6］ALTAF M A，NAZ S，KUMAR R，et al.Unraveling the mechanisms of cadmium toxicity in horticultural plants：Implications for plant health［J］.South African Journal of Botany，2023,163：433-442.［7］安婷婷,黄帝,王浩,等.植物响应镉胁迫的生理生化机制研究进展［J］.植物学报,2021,56(3):347-362.［8］GALLEGO S M，PENA L B，BARCIA R A，et al.Unravelling cadmium toxicity and tolerance in plants：Insight into regulatory mechanisms［J］.Environmental and Experimental Botany，2012,83：33-46.［9］孙丽娟,段德超,彭程,等.硫对土壤重金属形态转化及植物有效性的影响研究进展［J］.应用生态学报,2014,25(7):2141-2148.［10］赵萌.硫对水稻根区微界面有效态镉的影响及机制［D］.北京:中国农业科学院,2021.［11］周彩玉,林隽,唐钰焱,等.硫肥对水稻幼苗生长及砷、镉和铬累积的影响［J］.农业环境科学学报,2024,43(7):1458-1467.［12］张亚静,汪涛,郭巧生,等.野菊对Cd元素吸收特性及其对药材品质的影响［J］.中国中药杂志,2019,44(4):641-647.［13］刘晏廷,黄沁梅,何淼.野菊CibZIP43基因及其启动子的克隆与表达分析［J］.北方园艺,2025(11):23-31.［14］WU X,CHEN Q,CHEN L,et al.A WRKY transcription factor,PyWRKY75,enhanced cadmium accumulation and tolerance in poplar［J］.Ecotoxicology and Environmental Safety,2022,239:113630.［15］王学奎,黄见良.植物生理生化实验原理与技术［M］.3版.北京:高等教育出版社,2015.［16］宗子煖.硫对野菊缓解镉毒害的影响［D］.哈尔滨:东北林业大学,2022.［17］KELTJENS W G,van BEUSICHEM M L.phytochelatins as biomarkers for heavy metal stress in maize (Zea mays L.) and wheat (Triticum aestivum L.):Combined effects of copper and cadmium［J］.Plant and Soil,1998,203(1):119-126.［18］BHARGAVA P,KUMAR SRIVASTAVA A,URMIL S,et al.Phytochelatin plays a role in UV-B tolerance in N2-fixing Cyanobacterium Anabaena doliolum［J］.Journal of Plant physiology,2005,162(11):1220-1225.［19］李娜,王剑峰,龚记熠,等.植物响应镉胁迫的生理、分子和根际微生态机制研究进展［J］.江苏农业科学,2024,52(24):1-11.［20］TANG Z,WANG H Q,CHEN J,et al.Molecular mechanisms underlying the toxicity and detoxification of trace metals and metalloids in plants［J］.Journal of Integrative Plant Biology,2023,65(2):570-593.［21］ABE T,FUKAMI M,OGASAWARA M.Cadmium accumulation in the shoots and roots of 93 weed species［J］.Soil Science and Plant Nutrition,2008,54(4):566-573.［22］安志装,王校常,严蔚东,等.镉硫交互处理对水稻吸收累积镉及其蛋白巯基含量的影响［J］.土壤学报,2004,41(5):728-734.［23］MATRASZEK-GAWRON R,HAWRYLAK-NOWAK B.Sulfur nutrition level modifies the growth,micronutrient status,and cadmium distribution in cadmium-exposed spring wheat［J］.Physiology and Molecular Biology of Plants,2019,25(2):421-432.［24］黄玉山,罗广华,关棨文.镉诱导植物的自由基过氧化损伤［J］.Acta Botanica Sinica,1997(6):522-526.［25］LI C,CAO Y,LI T,et al.Changes in antioxidant system and sucrose metabolism in maize varieties exposed to Cd［J］.Environmental Science and Pollution Research,2022,29(43):64999-65011.［26］范旭杪,秦丽,王吉秀,等.植物谷胱甘肽代谢与镉耐性研究进展［J］.西部林业科学,2019,48(4):50-56.［27］LI X,ZHANG L,LI Y,et al.Changes in photosynthesis,antioxidant enzymes and lipid peroxidation in soybean seedlings exposed to UV-B radiation and/or Cd［J］.Plant and Soil,2012,352(1):377-387.［28］YANG Z，XUE B，SONG G，et al.Effects of citric acid on antioxidant system and carbon-nitrogen metabolism of Elymus dahuricus under Cd stress［J］.Ecotoxicology and Environmental Safety，2022,233：113321.［29］HERNNDEZ L E,SOBRINO-PLATA J,MONTERO-PALMERO M B,et al.Contribution of glutathione to the control of cellular redox homeostasis under toxic metal and metalloid stress［J］.Journal of Experimental Botany,2015,66(10):2901-2911.［30］李欣宇.Cd胁迫下不同积累型小麦品种筛选及外源硅施用效果差异研究［D］.杨凌:西北农林科技大学,2025.［31］HUANG Y,CHEN J,SUN Y,et al.Mechanisms of calcium sulfate in alleviating cadmium toxicity and accumulation in pak choi seedlings［J］.Science of the Total Environment,2022,805:150115.
相似文献/References:
[1]韩 磊,杨 兴 芳,丁 雪 珍,等.野 菊 的 扩 繁 技 术 研 究[J].北方园艺,2010,34(21):0.[doi:10.11937/bfyy.201021033]
HAN Lei,YANG Xing -fang,DING Xue -zhen,et al.Study on Multiplication T echnology of Wild Chrysanthemum[J].Northern Horticulture,2010,34(8):0.[doi:10.11937/bfyy.201021033]
[2]韩 磊,吕 鑫,张 文 静,等.野菊花蕾的组培与快繁技术研究[J].北方园艺,2009,33(05):0.[doi:10.11937/bfyy.200905037]
HAN Lei,LV Xin,ZHANG Wen - jing,et al.Study on Flower BudTissue Culture andRapid Propagating T echnique of Chrysanthemum indicum L.[J].Northern Horticulture,2009,33(8):0.[doi:10.11937/bfyy.200905037]
[3]周 杰,陈 俊 愉.十一份不同地理居群野菊的 ISSR 分析[J].北方园艺,2009,33(08):0.[doi:10.11937/bfyy.200908073]
ZHOU Jie,CHEN Jun - yu.Genetic Diversity Revealed by ISSR Marker in Chrysanthemumindicum of Different Geographical Distribution[J].Northern Horticulture,2009,33(8):0.[doi:10.11937/bfyy.200908073]
[4]刘晏廷,黄沁梅,何淼.野菊CibZIP43基因及其启动子的克隆与表达分析[J].北方园艺,2025,(11):23.[doi:10.11937/bfyy.20244533]
LIU Yanting,HUANG Qinmei,HE Miao.Cloning and Expression Analysis of CibZIP43 Gene and Its Promoter in Chrysanthemum indicium[J].Northern Horticulture,2025,(8):23.[doi:10.11937/bfyy.20244533]
[5]宗子煖,倪星雨,陈楚怡,等.外源硫对镉胁迫下不同耐镉性野菊光合特性的影响[J].北方园艺,2023,(14):56.[doi:10.11937/bfyy.20225043]
ZONG Zixuan,NI Xingyu,CHEN Chuyi,et al.Effects of Exogenous Sulfur on Photosynthetic Characteristics of Chrysanthemum indicum With Different Cadmium Tolerance Under Cadmium Stress[J].Northern Horticulture,2023,(8):56.[doi:10.11937/bfyy.20225043]
备注/Memo
第一作者简介：田荷婷(2000-)，女，硕士研究生，研究方向为园林植物种质资源的开发与利用。E-mail:1248372695@qq.com.责任作者：岳莉然（1978-），女，博士，副教授，现主要从事园林植物种质资源的开发与利用等研究工作。E-mail:ms_yueliran@163.com.基金项目：黑龙江省自然科学基金资助项目（LH2021C018）。收稿日期：2025-10-11