[1]曹辉,张晓申,左红娟,等.NAC转录因子家族在金银花黄花和白花中的差异分析[J].北方园艺,2026,(8):30-39.[doi:10.11937/bfyy.20253508]
　CAO Hui,ZHANG Xiaoshen,ZUO Hongjuan,et al.Analysis of the Differences of the NAC Transcript Factor Family in Yellow and White Flowers of Lonicera japonica[J].Northern Horticulture,2026,(8):30-39.[doi:10.11937/bfyy.20253508]
点击复制

NAC转录因子家族在金银花黄花和白花中的差异分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年8 页码: 30-39 栏目: 出版日期: 2026-04-30
Title:
Analysis of the Differences of the NAC Transcript Factor Family in Yellow and White Flowers of Lonicera japonica
文章编号:
1001-0009(2026)08-0030-10
作者:
曹辉1; 张晓申1; 左红娟1; 王峰2; 张三龙3; 牛苏燕3
（1.郑州市农业科技研究院，河南 郑州 450005；2.郑州市农业技术推广中心，河南 郑州 450000；3.郑州师范学院，河南 郑州 450044）
Author(s):
CAO Hui1; ZHANG Xiaoshen1; ZUO Hongjuan1; WANG Feng2; ZHANG Sanlong3; NIU Suyan3
(1.Zhengzhou Academy of Agricultural Science and Technology,Zhengzhou,Henan 450005;2.Zhengzhou Agricultural Technology Extension Center,Zhengzhou,Henan 450000;3.Zhengzhou Normal University,Zhengzhou,Henan 450044)
关键词:
NAC基因; 金银花; 黄花和白花; 生物信息学分析
Keywords:
NAC gene; Lonicera japonica; yellow and white flowers; bioinformatics analysis
分类号:
S567.79
DOI:
10.11937/bfyy.20253508
文献标志码:
A
摘要:
以金银花“郑银花3号”黄花和白花花朵转录组数据为试材，采用生物信息学分析方法，研究了NAC基因在不同花色（黄花和白花）中的表达模式及其潜在的调控机制，以期揭示这些基因在金银花花色形成中的作用，为进一步研究金银花NAC基因家族的功能提供参考依据。结果表明：该品种金银花黄花和白花中NAC转录因子家族有58个成员，氨基酸数目为44~737个，平均相对分子质量为38 347.79 Da，绝大多数NAC转录因子呈酸性，少数为碱性，所有的NAC蛋白均为亲水蛋白；序列分析结果显示，58个金银花NAC家族成员共包含10个motif，其中大部分家族成员中均含有motif 1、motif 2、motif 3、motif 4、motif 5、motif 6、motif 7和motif 10，其中绝大多数motif 5和motif 3相连存在；Lj4A633T23在金银花白花和黄花中的表达量最大，在白花中表达量高于在黄花中的表达量，其次是Lj1A850G59，其在白花中表达量显著低于黄花；GO功能注释表明，58个NAC转录因子参与了8种生物过程，2种分子功能和3种细胞组分。
Abstract:
Taking the transcriptome data of yellow and white flowers of the honeysuckle variety ‘Zhengyinhua 3’ as experimental materials，bioinformatics analysis methods were employed to study the expression patterns and potential regulatory mechanisms of NAC genes in different flower colors (yellow and white)，in order to reveal their roles in honeysuckle flower color formation and provide references for further research on the NAC gene family in honeysuckle.The results showed that the NAC transcription factor family in this honeysuckle variety had 58 members，with amino acid numbers ranging from 44 to 737 and an average relative molecular weight of 38 347.79 Da.The majority of NAC transcription factors were acidic，while a few were alkaline，and all NAC proteins were hydrophilic.Sequence analysis revealed that the 58 NAC family members in honeysuckle contained 10 motifs，with most family members harboring motifs 1，2，3，4，5，6，7，and 10.Among them，motif 5 and motif 3 were mostly connected.Lj4A633T23 exhibited the highest expression levels in both white and yellow honeysuckle flowers，with higher expression in white flowers than in yellow ones，followed by Lj1A850G59，which showed significantly lower expression in white flowers compared to yellow ones.GO functional annotation indicated that the 58 NAC transcription factors participated in 8 biological processes，2 molecular functions，and 3 cellular components.

参考文献/References:

［1］高琪,张德斌,高韶晨,等.叶面喷施亚硒酸钠对金银花有效成分含量及品质的影响［J］.北方园艺,2024(18):92-99.［2］付林江.金银花花色变化的原因及其药材品质分析［D］.杨凌:西北农林科技大学,2013.［3］AIDA M,ISHIDA T,FUKAKI H,et al.Genes involved in organ separation in Arabidopsis:An analysis of the cup-shaped Cotyledon mutant［J］.The Plant Cell,1997,9(6):841-857.［4］陆海芹,李娜,蒋凯旋,等.NAC转录因子调控植物生长发育和胁迫应答的研究进展［J］.植物生理学报,2024,60(2):271-283.［5］于婧瑶,巩琛锐.植物NAC转录因子的生物学功能研究进展［J］.河南科学,2024,42(11):1589-1601.［6］OOKA H,SATOH K,DOI K,et al.Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana［J］.DNA Research,2003,10(6):239-247.［7］SOUER E,van HOUWELINGEN A,KLOOS D,et al.The No apical meristem gene of Petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries［J］.Cell,1996,85(2):159-170.［8］王佳丽,王鹤冰,杨慧勤,等.NAC转录因子在植物花发育中的作用［J］.生物工程学报,2022,38(8):2687-2699.［9］李捷,陈旭,罗莉琼,等.ANAC092参与调控花药发育的功能初探［J］.遗传,2013,35(7):913-922.［10］ZHANG Z B,ZHU J,GAO J F,et al.Transcription factor AtMYB103 is required for anther development by regulating tapetum development,callose dissolution and exine formation in Arabidopsis［J］.The Plant Journal,2007,52(3):528-538.［11］MITSUDA N,SEKI M,SHINOZAKI K,et al.The NAC transcription factors NST1 and NST2 of Arabidopsis regulate secondary wall thickenings and are required for anther dehiscence［J］.The Plant Cell,2005,17(11):2993-3006.［12］ZHONG R,RICHARDSON E A,YE Z H.Two NAC domain transcription factors,SND1 and NST1,function redundantly in regulation of secondary wall synthesis in fibers of Arabidopsis［J］.Planta,2007,225(6):1603-1611.［13］杨应杰,张付昆,穆静怡,等.矮牵牛花瓣衰老和逆境胁迫响应相关NAC基因的鉴定与分析［J］.福建农业学报,2024,39(6):700-710.［14］ZHU M K，CHEN G P，ZHOU S，et al.A new tomato NAC (N AM/ATAF1/2/C UC2) transcription factor，SlNAC4，functions as a positive regulator of fruit ripening and carotenoid accumulation［J］.Plant Cell Physiology,2014,55(1):119-135.［15］冯源,刘烨方,寇晓虹,等.CRISPR/Cas9靶向敲除番茄SNAC4/9突变体的构建、鉴定及分析［J］.核农学报,2023,37(2):217-229.［16］ZHANG S Y，CHEN Y X，ZHAO L L，et al.A novel NAC transcription factor，MdNAC42，regulates anthocyanin accumulation in red-fleshed apple by interacting with MdMYB10［J］.Tree Physiology，2020,40，413-423.［17］AN X H，TIAN Y，CHEN K Q，et al.MdMYB9 and MdMYB11 are involved in the regulation of the JA-induced biosynthesis of anthocyanin and proanthocyanidin in apples［J］.Plant and Cell Physiology，2015，56(4)：650-662.［18］舒琳,李龙辉,曹亚男,等.甘蓝型油菜NAC069基因的克隆与功能分析［J］.农业生物技术学报,2024,32(12):2701-2714.［19］王敬,崔画,谢岩,等.不同花色黄芩花色苷合成转录调控分析［J］.北方园艺,2025(15):12-20.［20］LE D T,NISHIYAMA R,WATANABE Y,et al.Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress［J］.DNA Research,2011,18(4):263-276.［21］张忠鑫,郭夕雯,汪泽文,等.欧李NAC基因家族的鉴定及表达分析［J］.果树学报,2023,40(2):206-222.［22］GRANT E H,FUJINO T,BEERS E P,et al.Characterization of NAC domain transcription factors implicated in control of vascular cell differentiation in Arabidopsis and Populus［J］.Planta,2010,232(2):337-352.［23］PENG X,ZHAO Y,LI X,et al.Genomewide identification,classification and analysis of NAC type gene family in maize［J］.Journal of Genetics,2015,94(3):377-390.［24］ZHU X,CHEN J,XIE Z,et al.Jasmonic acid promotes degreening via MYC2/3/4-and ANAC019/055/072-mediated regulation of major chlorophyll catabolic genes［J］.The Plant Journal,2015,84(3):597-610.［25］LI S,GAO J,YAO L,et al.The role of ANAC072 in the regulation of chlorophyll degradation during age-and dark-induced leaf senescence［J］.Plant Cell Reports,2016,35(8):1729-1741.［26］FUJITA M,FUJITA Y,MARUYAMA K,et al.A dehydration-induced NAC protein,RD26,is involved in a novel ABA-dependent stress-signaling pathway［J］.The Plant Journal,2004,39(6):863-876.［27］ZHAO C,CRAIG J C,PETZOLD H E,et al.The xylem and phloem transcriptomes from secondary tissues of the Arabidopsis Root-hypocotyl［J］.Plant Physiology,2005,138(2):803-818.［28］ZHAO C,AVCI U,GRANT E H,et al.XND1,a member of the NAC domain family in Arabidopsis thaliana,negatively regulates lignocellulose synthesis and programmed cell death in xylem［J］.The Plant Journal,2008,53(3):425-436.［29］TANG N,SHAHZAD Z,LONJON F,et al.Natural variation at XND1 impacts root hydraulics and trade-off for stress responses in Arabidopsis［J］.Nature Communications,2018,9:3884.［30］ZHOU J,ZHONG R,YE Z H.Arabidopsis NAC domain proteins,VND1 to VND5,are transcriptional regulators of secondary wall biosynthesis in vessels［J］.PLoS One,2014,9(8):e105726.［31］LEE S,LEE H J,HUH S U,et al.The Arabidopsis NAC transcription factor NTL4 participates in a positive feedback loop that induces programmed cell death under heat stress conditions［J］.Plant Science,2014,227:76-83.

相似文献/References:

[1]王惠利,赵晓明.四倍体金银花的细胞学鉴定[J].北方园艺,2014,38(06):92.
　WANG Hui-li,ZHAO Xiao-ming.Cytology Identification of Tetraploid Lonicera japonica Thunb[J].Northern Horticulture,2014,38(8):92.
[2]张建海,冯彬彬,牛小花,等.金银花在三峡库区的引种试验[J].北方园艺,2014,38(08):138.
　ZHANG Jian-hai,FENG Bin-bin,NIU Xiao hua,et al.Introduction Experiment on Lonicerae japonicae Flos in Three Gorges Reservoir Area[J].Northern Horticulture,2014,38(8):138.
[3]王玉珍.不同品种金银花在盐碱地引种栽培对比研究[J].北方园艺,2013,37(15):157.
　WANG Yu-zhen.Comparison Test of Different Varieties of Lonicera japonica Introduced and Planted in Saline alkali Soil[J].Northern Horticulture,2013,37(8):157.
[4]单广福,张晓丽,李明军,等.金银花愈伤组织的诱导及继代培养研究[J].北方园艺,2013,37(05):106.
　SHAN Guang-fu,ZHANG Xiao-li,LI Ming-jun,et al.Study on Callus Induction and Subculture of Lonicera japonica Thunb.[J].Northern Horticulture,2013,37(8):106.
[5]岑怡红,沙波,张万芹,等.金银花立枯病的发生特点及防治措施[J].北方园艺,2013,37(08):135.
　CEN Yi-hong,SHA Bo,ZHANG Wan-qin,et al.Occurrence Characteristics and Control Methods of Lonicera japonic Thunb.Seedling Blight[J].Northern Horticulture,2013,37(8):135.
[6]廖林正,周友兵,吴涤,等.生长调节物质及木质化程度对金银花扦插繁育的影响[J].北方园艺,2012,36(04):161.
　LIAO Lin-zheng,ZHOU You-bing,WU Di,et al.Effect of Lignification Degree and Growth Regulating Substance on Shoot Cutting of Honeysuckle[J].Northern Horticulture,2012,36(8):161.
[7]武丽娜.金银花疏松愈伤组织诱导及细胞悬浮培养研究[J].北方园艺,2012,36(20):107.
　WU Li-na.Study on the Loose Callus Induction and Cell Suspension Culture of Lonicera japonica[J].Northern Horticulture,2012,36(8):107.
[8]徐永忠,李莲,司剑华,等.高原寒旱条件下金银花种植技术[J].北方园艺,2012,36(19):173.
　XU Yong-zhong,LI Lian,SI Jian-hua,et al.Cultivation Technology of Honeysuckle in Plateau Cold and Arid Conditions[J].Northern Horticulture,2012,36(8):173.
[9]王海亭.不同产地金银花抗寒性分析[J].北方园艺,2014,38(23):139.
　WANG Hai-ting.The Analysis of Cold Resistance of Honeysuckle in Different Habitats[J].Northern Horticulture,2014,38(8):139.
[10]孟衡玲,张 薇,卢丙越,等.铜胁迫对金银花幼苗生理指标的影响[J].北方园艺,2015,39(05):167.[doi:10.11937/bfyy.201505048]
　MENG Heng-ling,ZHANG Wei,LU Bing-yue,et al.Effect of Copper Stress on Physiological Indexes in Lonicera japonica Seedling[J].Northern Horticulture,2015,39(8):167.[doi:10.11937/bfyy.201505048]

备注/Memo

第一作者简介：曹辉（1977-），女，硕士，副研究员，现主要从事药食同源中药材新品种选育及栽培等研究工作。E-mail:caoh166@126.com.责任作者：张晓申（1976-），男，博士，副研究员，现主要从事中药材新品种选育及栽培等研究工作。E-mail:nkszyc@126.com.基金项目：河南省林木科技兴林资助项目（豫财环资［2025］44号）。收稿日期：2025-10-10

更新日期/Last Update: 2026-05-06