[1]牛少君,杨帆,洪煌楷,等.菊花CmbZIP53基因克隆与表达分析[J].北方园艺,2026,(6):10-17.[doi:10.11937/bfyy.20253405]
　NIU Shaojun,YANG Fan,HONG Huangkai,et al.Cloning and Expression Analysis of the CmbZIP53 Gene in Chrysanthemum morifolium[J].Northern Horticulture,2026,(6):10-17.[doi:10.11937/bfyy.20253405]
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菊花CmbZIP53基因克隆与表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年6 页码: 10-17 栏目: 出版日期: 2026-03-30
Title:
Cloning and Expression Analysis of the CmbZIP53 Gene in Chrysanthemum morifolium
文章编号:
1001-0009(2026)06-0010-08
作者:
牛少君1; 2; 杨帆1; 2; 洪煌楷1; 2; 袁美佳1; 2; 赵楠1; 2
（1．闽南师范大学 生物科学与技术学院，福建 漳州 363000；2．闽南师范大学 闽台特色园林植物福建省高校重点实验室，福建 漳州 363000）
Author(s):
NIU Shaojun1; 2; YANG Fan1; 2; HONG Huangkai1; 2; YUAN Meijia1; 2; ZHAO Nan1; 2
(1.School of Biological Science and Technology，Minnan Normal University，Zhangzhou，Fujian 363000；2.Key Laboratory of Landscape Plants With Fujian and Taiwan Characteristics of Fujian Colleges and Universities，Minnan Normal University，Zhangzhou，Fujian 363000)
关键词:
菊花; CmbZIP53; 基因克隆; 表达模式
Keywords:
Chrysanthemum morifolium; CmbZIP53; gene cloning; expression pattern
分类号:
S682.1+1
DOI:
10.11937/bfyy.20253405
文献标志码:
A
摘要:
以菊花（Chrysanthemum morifolium）‘神马’品种为试材，根据菊花淹水转录组，克隆CmbZIP53基因及其启动子序列，对其进行生物信息学和淹水表达分析，探究CmbZIP53在淹水胁迫响应中可能的作用机制，以期为进一步研究菊花bZIP转录因子响应淹水胁迫的功能以及耐涝关键基因的发掘提供参考依据。结果表明：CmbZIP53基因开放阅读框长度为417 bp，编码138个氨基酸；蛋白结构预测发现CmbZIP53含有BRLZ结构域，属于bZIP蛋白家族，与野菊CibZIP29蛋白亲缘关系最近；该基因显著受淹水胁迫诱导，且随淹水时间的延长CmbZIP53诱导水平进一步增强；启动子片段中含有低氧等胁迫响应元件、激素信号响应元件等多个顺式元件，表明CmbZIP53可能参与菊花的淹水响应过程。
Abstract:
Taking the Chrysanthemum morifolium cultivar ‘Jinba’ as experimental material，the CmbZIP53 gene and its promoter sequence were cloned based on the transcriptomic data of C.morifolium under waterlogging stress.Bioinformatic analysis and waterlogging expression analysis were conducted to explore the potential role of CmbZIP53 in the response to waterlogging stress，in order to provide reference for further research on the function of bZIP transcription factors and to identify key genes related to waterlogging tolerance in C.morifolium.The results showed that the length of open reading frame (ORF) of CmbZIP53 was 417 bp，encoding 138 amino acids.Protein structure prediction revealed that CmbZIP53 contained a typical BRLZ domain of bZIP proteins，indicating its belonging to the bZIP protein family，with the closest relationship to the CibZIP29 protein of Chrysanthemum indicum.Waterlogging stress significantly induced the expression of CmbZIP53，and its induction level progressively increased with prolonged waterlogging duration.The promoter sequence was found to contain multiple cis-acting elements，including anaerobic induction-responsive elements and hormone signaling-responsive elements，suggesting that CmbZIP53 may be involved in the waterlogging response process in C.morifolium.

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备注/Memo

第一作者简介：牛少君（2000-），女，硕士研究生，研究方向为园林植物。E-mail：nsj3442@163.com.责任作者：赵楠（1990-），男，博士，讲师，现主要从事园林植物等研究工作。E-mail：zhaonan21@163.com.基金项目：国家自然科学基金资助项目（32401659）；福建省自然科学基金资助项目（2022J01907）；闽南师范大学校长基金资助项目（KJ2020005）。收稿日期：2025-09-26

更新日期/Last Update: 2026-04-01