CHEN Xuan,ZHANG Ziyang,WU Yixin,et al.Identification and Analysis of TCP Gene Family in the Round-toothed Wild Crow[J].Northern Horticulture,2026,(9):35-43.[doi:10.11937/bfyy.20253099]
圆齿野鸦椿TCP基因家族鉴定及分析
- Title:
- Identification and Analysis of TCP Gene Family in the Round-toothed Wild Crow
- 文章编号:
- 1001-0009(2026)09-0035-09
- 分类号:
- S567.19
- 文献标志码:
- A
- 摘要:
- 以我国特有药用植物圆齿野鸦椿为试材，采用生物信息学方法，在圆齿野鸦椿全基因组水平上研究了TCP基因家族成员的理化性质、系统进化关系、基因结构、保守基序、启动子顺式作用元件及其在不同组织中的表达模式，以期为圆齿野鸦椿生长发育调控机制研究提供参考依据。结果表明：共鉴定出27个EkTCP基因，分属PCF、CYC和CIN 3个亚家族，在9条染色体上呈不规则分布。这些基因的氨基酸数量为82~442个，相对分子质量9 217.62~50 394.6，均为亲水蛋白。顺式作用元件分析显示其含多种与植物生长发育、光响应、激素调节及黄酮类生物合成调控相关的元件，其中黄酮类生物合成调控响应元件。基因表达热图显示，EkTCP13、EkTCP5伴随着果实的发育，表达量逐渐上升，可能参与果实成熟调控；EkTCP2在整花中呈现高表达，可能对花的形成具有促进作用。这些发现揭示了TCP基因家族在协调圆齿野鸦椿叶片发育与果实成熟中的系统性调控作用，为理解植物生殖生长分子机制提供了新视角。
- Abstract:
- Taking the medicinal plants unique to China,the TCP gene was identified and analyzed using bioinformatics methods to investigate its role in the growth and development of the Round toothed Wild Crow Tree.The results showed that the amino acid numbers ranged from 82 to 442，with molecular weights between 9 217.62 and 50 394.6.All of them were hydrophilic proteins，furthermore，cis-acting element analysis revealed that the promoter regions of these genes harbored numerous regulatory elements associated with plant growth and development，light response，hormone signaling，and flavonoid biosynthesis.The gene expression heatmap showed that the expression levels of EkTCP13 and EkTCP5 progressively increased during fruit development，implying their potential roles in fruit ripening regulation.In contrast，EkTCP2 was highly expressed in floral organs，suggesting its possible positive regulatory role in flower morphogenesis.In summary，our findings elucidated the systematic regulatory role of the TCP gene family in coordinating leaf development and fruit maturation in E.japonica，providing an important theoretical foundation for further understanding the molecular mechanisms underlying plant reproductive growth.
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备注/Memo
第一作者简介：陈烜(1999-)，男，硕士研究生，研究方向为药用植物开发与利用。E-mail：1565869543@qq.com.责任作者：邹双全(1963-)，男，本科，研究员，博士生导师，现主要从事森林培育、观赏药用植物等研究工作。E-mail：zou@fafu.edu.cn.基金项目：福建省科技计划引导性资助项目（2022Y0043）；福建省科技计划高校产学专项资助项目(2020N5004）；中央财政林业科技推广专项资助项目（闽2025TG04号）。收稿日期：2025-09-09