DANG Yongxiang,JIANG Changsheng,YANG Qin,et al.Elucidation of the Toxicological Mechanism of Herbicides in Morchella sextelata Based on Transcriptomic Analysis[J].Northern Horticulture,2026,(6):33-39.[doi:10.11937/bfyy.20253079]
基于转录组分析除草剂对羊肚菌的毒理机制
- Title:
- Elucidation of the Toxicological Mechanism of Herbicides in Morchella sextelata Based on Transcriptomic Analysis
- 文章编号:
- 1001-0009(2026)06-0033-07
- Keywords:
- Morchella sextelata; herbicides; transcriptomic analysis; toxicology
- 分类号:
- S646.7
- 文献标志码:
- A
- 摘要:
- 以‘六妹’羊肚菌（Morchella sextelata）M6611菌株为试材，采用PDA平板培养法并辅以RNA-seq转录组分析，研究了4种常用除草剂（氟乐灵、二甲戊灵、精异丙甲草胺和乙草胺）对其菌丝生长的影响，以期为分析除草剂对羊肚菌的毒理机制提供参考依据。结果表明：所有除草剂均抑制菌丝生长，其中精异丙甲草胺抑制程度相对较轻；转录组分析显示除草剂处理显著干扰糖酵解/糖异生及乙醛酸循环等与能量代谢密切相关的通路。因此，在羊肚菌栽培中应谨慎使用除草剂，并加强土壤除草剂残留监测，以减少菌丝生长受阻和产量下降的潜在风险。
- Abstract:
- Taking the Morchella sextelata strain M6611 as the test material,the PDA plate culture method was adopted and combined with RNA-seq transcriptome analysis to study the effects of four commonly used herbicides (trifluralin,pendimethalin,S-metolachlor and acetochlor)) on the mycelial growth of the mushroom,in order to provide references for analyzing the toxicological mechanism of herbicides on morel mushrooms.The results showed that all herbicides suppressed mycelial growth，with S-metolachlor exhibiting relatively weaker inhibition.Comprehensive transcriptome analysis revealed that herbicide treatment severely impaired critical energy metabolism pathways，particularly the processes of glycolysis/gluconeogenesis and the glyoxylate cycle.In conclusion，herbicides should be used cautiously in M.sextelata cultivation，and soil herbicide residue monitoring should be enhanced to reduce potential risks of mycelial growth inhibition and yield loss.
参考文献/References:
［1］BADALYAN S，GHARIBYAN N，GIANCHINO C，et al.Morphological observation and biomass formation in different edible medicinal Morchella collections (Pezizomycetes，Ascomycota)［J］.Italian Journal of Mycology，2023，52：50-61.［2］FENG B，YANG Z.Studies on diversity of higher fungi in Yunnan,southwestern China：A review［J］.Plant Diversity，2018，40(4)：165-171.［3］罗燕娜，王静静，李旭东，等.新疆五家渠地区羊肚菌高产栽培关键技术［J］.北方园艺，2025(3)：154-156.［4］LEDUY A，KOSARIC N，ZAJIC J E.Morel mushroom Mycelium growth in waste sulfite liquors as source of protein and flavouring［J］.Canadian Institute of Food Science and Technology Journal，1974，7(1)：44-50.［5］LI J，WU H，LIU Y，et al.The chemical structure and immunomodulatory activity of an exopolysaccharide produced by Morchella esculenta under submerged fermentation［J］.Food & Function，2021，12(19)：9327-9338.［6］LIU C，SUN Y，MAO Q，et al.Characteristics and antitumor activity of Morchella esculenta polysaccharide extracted by pulsed electric field［J］.International Journal of Molecular Sciences，2016，17(6)：986.［7］FU L，WANG Y，WANG J，et al.Evaluation of the antioxidant activity of extracellular polysaccharides from Morchella esculenta［J］.Food & Function，2013，4(6)：871-879.［8］DU X H，ZHAO Q，YANG Z L.A review on research advances，issues，and perspectives of morels［J］.Mycology，2015，6(2)：78-85.［9］OWER R.Notes on the development of the morel ascocarp：Morchella esculenta［J］.Mycologia，1982，74(1)：142-144.［10］王晓敏，张钦语，陈波，等.五种重金属胁迫对‘六妹’羊肚菌菌丝体的影响［J］.北方园艺，2025(10)：78-85.［11］TAN H，LIU T，YU Y，et al.Morel production related to soil microbial diversity and evenness［J］.Microbiology Spectrum，2021，9(2)：e00229-e00221.［12］CARVALHO F P.Agriculture，pesticides，food security and food safety［J］.Environmental Science & Policy，2006，9(7/8)：685-692.［13］BLOSSEY B，NOTZOLD R.Evolution of increased competitive ability in invasive nonindigenous plants：A hypothesis［J］.Journal of Ecology，1995，83(5)：887-889.［14］SILVA V，MOL H G J，ZOMER P，et al.Pesticide residues in European agricultural soils：A hidden reality unfolded［J］.Science of the Total Environment，2019，653：1532-1545.［15］CURRAN W S.Persistence of herbicides in soil［J］.Crops & Soils，2016，49(5)：16-21.［16］RASHID B，HUSNAIN T，RIAZUDDIN S.Herbicides and pesticides as potential pollutants：A global problem［M］.ASHRAF M.Plant Adaptation and Phytoremediation.Dordrecht：Springer Netherlands，2010.［17］LING L，WANG Y，LI J，et al.Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases［J］.Food Bioscience，2024，58：103743.［18］GRABHERR M G，HAAS B J，YASSOUR M，et al.Full-length transcriptome assembly from RNA-Seq data without a reference genome［J］.Nature Biotechnology，2011，29(7)：644-652.［19］CAO X，LIU S，YANG X，et al.A modified QuEChERS sample preparation method for simultaneous determination of 62 pesticide residues in edible fungi using gas chromatography-triple quadrupole mass spectrometry［J］.Food Analytical Methods，2016，9(1)：263-274.［20］MUKAH F E，CHINEDU-NDUKWE P A，IMARHIAGBE O，et al.Agrochemical use and emerging human and animal diseases［M］.OGWU M C.One Health Implications of Agrochemicals and their Sustainable Alternatives［M］.Singapore：Springer Nature Singapore，2023.
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备注/Memo
第一作者简介：党永祥（1999-），男，硕士研究生，研究方向为大型真菌资源与分类。E-mail：2023212768@nwnu.edu.cn.责任作者：朱学泰（1979-），男，博士，教授，现主要从事大型真菌资源与分类等研究工作。E-mail：zhuxuetai@nwnu.edu.cn.基金项目：国家自然科学基金资助项目(31770588)；2024年度兰州市第八批科技计划资助项目(2024-8-26)。收稿日期：2025-08-27