ZHANG Huimin,FENG Lulu,WANG Manman,et al.Pathogen Identification and Germplasm Screening of Melon Downy Mildew Disease[J].Northern Horticulture,2026,(5):126-132.[doi:10.11937/bfyy.20253062]
甜瓜霜霉病病原鉴定及抗病种质筛选
- Title:
- Pathogen Identification and Germplasm Screening of Melon Downy Mildew Disease
- 文章编号:
- 1001-0009(2026)05-0126-07
- Keywords:
- melon; downy mildew; pathogen identification
- 分类号:
- S652
- 文献标志码:
- A
- 摘要:
- 以采集的徐州市(XZ)、淮安市(HA)、苏州市(SZ)及南通市(NT)四地甜瓜霜霉病菌为试材,采用田间病害识别,病原显微形态观察及ITS序列分析等方法构建了系统发育树,研究了病原菌分类,明确江苏省甜瓜霜霉病病原菌类型,以期为甜瓜抗霜霉病种质选育及抗病机理研究提供参考依据。结果表明:4个地区甜瓜霜霉病菌序列相似度高达99%,均属于Pseudoperonospora cubensis;此外,课题组以收集的204份甜瓜种质为试材,开展了苗期和成株期霜霉病抗病性鉴定。综上,204份甜瓜种质霜霉病抗性筛选出高抗霜霉病种质3份(554、20144和1234),抗霜霉病种质40份,中抗霜霉病种质113份。
- Abstract:
- Taking the downy mildew pathogen of melon collected from four places,namely Xuzhou (XZ),Huai′an (HA),Suzhou (SZ) and Nantong (NT),as the test materials,by using methods such as field disease identification,microscopic morphology observation of the pathogen and ITS sequence analysis,a phylogenetic tree was constructed to study the classification of the pathogen,and to clarify the types of downy mildew pathogen of melon in Jiangsu Province,in order to provide reference for breeding downy mildew-resistant melon germplasm and investigating disease resistance mechanisms.The results showed that the sequence similarity of the downy mildew pathogen of melon in the four regions was as high as 99%,and all belonged to Pseudoperonospora cubensis.In addition,the research group used 204 melon germplasms collected as test materials to conduct disease resistance identification of downy mildew at the seedling stage and the mature plant stage.The results showed that among the 204 melon germplasms,3 varieties were screened out as highly resistant to downy mildew (554,20144 and 1234),40 varieties were moderately resistant to downy mildew,and 113 varieties were resistant to downy mildew.
参考文献/References:
[1]张凯歌,胡倩梅,靳志恒,等.219份甜瓜种质资源的遗传多样性分析[J].河南农业大学学报,2020,54(2):216-230.[2]王娟娟,李莉,尚怀国.我国西瓜甜瓜产业现状与对策建议[J].中国瓜菜,2020,33(5):69-73.[3]WENG J,LI P,REHMAN A,et al.Physiological response and evaluation of melon (Cucumis melo L.) germplasm resources under high temperature and humidity stress at seedling stage[J].Scientia Horticulturae,2021,288:110317.[4]COHEN Y,VAN DEN LANGENBERG K M,WEHNER T C,et al.Resurgence of Pseudoperonospora cubensis:The causal agent of cucurbit downy mildew[J].Phytopathology,2015,105(7):998-1012.[5]刘广,徐锦华,张曼,等.江苏省西甜瓜产业现状与发展对策[J].中国蔬菜,2021(11):6-11.[6]宋正峰,刘树森,夏连芹,等.甜瓜育种技术与方法研究进展[J].中国瓜菜,2022,35(6):1-8.[7]汪可心,李淑敏,孟繁君,等.吉林省甜瓜霜霉病的发生规律及综合防治方法[J].农业开发与装备,2017(12):165.[8]张保民.不同药剂防治甜瓜霜霉病药效试验研究[J].现代农业科技,2018(12):114,127.[9]任毓忠,翁晓梅,李国英,等.10种药剂防治甜瓜霜霉病的效果试验[J].石河子大学学报(自然科学版),2004,22(S1):101-103.[10]SAVORY E A,GRANKE L L,QUESADA-OCAMPO L M,et al.The cucurbit downy mildew pathogen Pseudoperonospora cubensis[J].Molecular Plant Pathology,2011,12(3):217-226.[11]张学军,郭丽霞,张健,等.甜瓜霜霉病苗期抗病性鉴定方法建立及种质资源评价[J].新疆农业科学,2019,56(5):899-909.[12]KUMAR A,BHARGAVA A,SHUKLA S,et al.Screening of exotic Chenopodium quinoa accessions for downy mildew resistance under mid-eastern conditions of India[J].Crop Protection,2006,25(8):879-889.[13]孙雪雯,盛云燕,靳亚忠,等.甜瓜细菌性果斑病病原菌的分离鉴定与抗性种质资源的筛选[J].北方园艺,2024(6):25-32.[14]COHEN Y.The combined effects of temperature,leaf wetness,and inoculum concentration on infection of cucumbers with Pseudoperonospora cubensis[J].Canadian Journal of Botany,1977,55(11):1478-1487.[15]张学军,郭丽霞,马新力,等.甜瓜种质资源抗霜霉病和蔓枯病苗期筛选与评价[J].中国瓜菜,2014,27(S1):38-40.[16]蔡宁.黑龙江省黄瓜霜霉病菌生理小种分化的研究[D].哈尔滨:东北农业大学,2008.[17]王娜,马雅军,代光辉,等.黄瓜霜霉病和白粉病病原菌的rDNA-ITS序列分析[J].西北农林科技大学学报(自然科学版),2007,35(10):155-158.[18]KUSABA M,TSUGE T.Phologeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA[J].Current Genetics,1995,28(5):491-498.[19]HIRATA T,TAKAMATSU S.Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi[J].Mycoscience,1996,37(3):283-288.[20]VOGLMAYR H.Phylogenetic relationships of Peronospora and related Genera based on nuclear ribosomal ITS sequences[J].Mycological Research,2003,107(10):1132-1142.[21]PINAT C,PITRAT M.Inheritance of resistance to downy mildew (Pseudoperonospora cubensis) in muskmelon (Cucumis melo).I.analysis of a 8×8 diallel table[J].Agronomie,1994,14(4):239-248.[22]吕淑珍,霍振荣,陈正武,等.黄瓜抗病性遗传研究初报[J].天津农林科技,1990(2):22-24.[23]彭丹丹,张源明,舒灿伟,等.植物病原真菌分子检测技术的研究进展[J].基因组学与应用生物学,2017,36(5):2015-2022.[24]白甜,黄大跃,刘璐,等.甜瓜霜霉病研究进展[J].江苏农业科学,2023,51(18):12-19.[25]COLUCCI S J.Host range fungicide resistance and management of Pseudoperonospora cubensis,Causal Agent of Cucurbit Downy Mildew[J/OL].Agricultural and Food Sciences,Environmental Sciences,Biology,(2008-08-13)[2025-08-26].http://www.lib.nscu.edu/resolver/1840.16/2975.[26]贺玉花,徐永阳,徐志红,等.甜瓜霜霉病抗性研究进展[J].果树学报,2014,31(2):324-334.
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备注/Memo
第一作者简介:张惠敏(1996-),女,硕士,助理研究员,现主要从事甜瓜霜霉病抗病育种等研究工作。E-mail:20220057@jaas.accn.责任作者:王康(1988-),男,硕士,副研究员,现主要从事甜瓜育种等研究工作。E-mail:wangkang@jaas.ac.cn.基金项目:南通市社会民生科技计划资助项目(MS2024044,MS2023054);江苏沿江地区农业科学研究所青年科技基金资助项目(YJ(2023)001);江苏沿江地区农业科学研究所学科建设基金资助项目(YJXK[2023]101)。收稿日期:2025-08-26