HUANG Houyun,WANG Fanfan,TANG Tao,et al.Pathogen Identification and Fungicide Efficacy for Preventive and Curative Control of Pinellia ternata Southern Blight[J].Northern Horticulture,2026,(3):93-101.[doi:10.11937/bfyy.20252899]
半夏白绢病病原菌鉴定及杀菌剂防治效果
- Title:
- Pathogen Identification and Fungicide Efficacy for Preventive and Curative Control of Pinellia ternata Southern Blight
- 文章编号:
- 1001-0009(2026)03-0093-09
- Keywords:
- Pinellia ternata southern blight; pathogen identification; Agroatheli arolfsii; biological characteristics; chemical prevention
- 分类号:
- S435.67
- 文献标志码:
- A
- 摘要:
- 以钟祥市半夏白绢病病害样本为试材，采用组织分离法、形态学与分子生物学鉴定、室内毒力测定、温室盆栽防治效果测定等方法，研究了8种杀菌剂对半夏白绢病病原菌的菌丝生长抑制效果及4种杀菌剂对该菌的预防和治疗效果，以期为半夏白绢病病害的预防管理提供参考依据。结果表明：半夏白绢病病原菌为罗氏阿太菌（Agroathelia rolfsii），命名为QJ3。罗氏阿太菌QJ3在pH、碳源和氮源的最适条件分别为6、可溶性淀粉、硝酸钾时，菌丝生长速率最快。8种药剂对QJ3均有不同程度的抑制效果，240 g·L-1 噻呋酰胺悬浮剂对QJ3的抑菌效果最佳，EC50值为0.228 2 μg·mL-1，70%咯菌腈水分散粒剂、25%吡唑醚菌酯悬浮剂和10%苯醚甲环唑水分散粒剂的抑菌效果较好，EC50值为0.615 1～0.877 2 μg·mL-1。4种杀菌剂的盆栽防治效表明25%吡唑醚菌酯悬浮剂的施用量为112.5 g·hm-2时，预防效果最好，为94.6%。10%苯醚甲环唑水分散粒剂在盆栽试验中的施用量为120 g·hm-2时，治疗效果最好，为77.59%。综上所述，防治罗氏阿太菌QJ3推荐使用25%吡唑醚菌酯悬浮剂和10%苯醚甲环唑水分散粒剂作为轮换药剂组合使用。
- Abstract:
- Taking disease samples of Pinellia ternata southern blight from Zhongxiang city as test materials,methods were employed including tissue isolation,morphological and molecular biological identification,indoor virulence determination,and greenhouse pot experiment for control efficacy evaluation.The inhibitory effects of eight fungicides on the mycelial growth of the pathogen causing Pinellia ternata southern blight,as well as the preventive and therapeutic effects of four fungicides against this pathogen were investigated,in order to provide a reference for the prevention and management of the disease.The results showed that the pathogen was identified as Agroathelia rolfsii,designated as strain QJ3.The optimal conditions for the mycelial growth rate of QJ3 were pH 6,soluble starch as the carbon source,and potassium nitrate as the nitrogen source.All eight fungicides exhibited varying degrees of inhibitory effects against QJ3.Among them,240 g·L-1 thifluzamide SC showed the strongest inhibition,with an EC50 value of 0.228 2 μg·mL-1.70% fludioxonil WG,25% pyraclostrobin SC,and 10% difenoconazole WG also demonstrated good inhibitory effects,with EC50 values ranging from 0.615 1 to 0.877 2 μg·mL-1.Pot experiments on the four fungicides indicated that 25% pyraclostrobin SC,applied at a rate of 112.5 g·hm-2,provided the best preventive effect,reaching 94.6%，10% difenoconazole WG,applied at 120 g·hm-2,showed the best therapeutic effect,achieving 77.59%.In conclusion,for the control of Pinellia ternata southern blight QJ3,it was recommended to use 25% pyraclostrobin SC and 10% difenoconazole WG in rotation.
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备注/Memo
第一作者简介：黄厚云(2000-)，男，硕士，研究实习员，现主要从事中药材病虫害绿色防控技术等研究工作。E-mail:3337029192@qq.com.责任作者：王明红(1980-),女，博士，副教授，现主要从事高山蔬菜病害绿色防控技术等研究工作。E-mail:red0799l@163.com.基金项目：湖北省重点研发计划资助项目（2024BBB090）；湖北自然科学基金创新发展联合基金资助项目（2024AFD081）；湖北省乡村振兴科技示范资助项目（2024BBB110）。收稿日期：2025-08-11