YUE Yuan,GENG Yuanyuan,ZHANG Shu,et al.Study on Microbial Diversity in Soils of Different Lycium barbarum Plantations in Ningxia[J].Northern Horticulture,2026,(3):118-127.[doi:10.11937/bfyy.20252826]
宁夏不同枸杞种植地土壤中微生物多样性研究
- Title:
- Study on Microbial Diversity in Soils of Different Lycium barbarum Plantations in Ningxia
- 文章编号:
- 1001-0009(2026)03-0118-10
- Keywords:
- Lycium barbarum; soil microorganism; microbial diversity; community structure; species variation analysis
- 分类号:
- S663.9
- 文献标志码:
- A
- 摘要:
- 以宁夏7个地区枸杞种植地土壤为试材，采用扩增子测序技术对其16S和ITS片段进行测序，对比不同区域土壤微生物群落的种类、数量、分布及丰度差异，同时结合温度、降水量、风沙及生态环境等环境因素进行综合分析。研究了宁夏地区枸杞种植土壤中微生物群落的组成及多样性分布特征，分析环境因素对土壤微生态差异的影响，以期为枸杞产业的健康发展提供参考依据。结果表明:7个区域枸杞种植地土壤中真菌丰度排名前5的分别为Fusarium、Neocosmospora、Botryotrichum、Mortierella、Fungi_cls_Incertae_sedis。不同区域枸杞土壤中细菌丰度排名前5的分别为norank_f__Gemmatimonadaceae、norank_f__Vicinamibacteraceae、norank_o__Vicinamibacterales、norank_f__A4b、MND1。多样性分析表明,红柳沟、舟塔和下马关3个区域群落组成相似性较高；7个区域的群落结构呈现真菌和细菌互补的关系；土壤中真菌和细菌的beta多样性均具有显著差异。宁夏不同枸杞种植区土壤微生物群落组成及多样性存在显著差异，且真菌和细菌的分布呈现互补关系。研究结果结合各地区温度、降水量、风沙及生态环境的因素，推测降水量对不同区域土壤中微生态的差异性具有重要影响。
- Abstract:
- Soil samples were collected from wolfberry planting areas across seven regions in Ningxia.Amplicon sequencing technology was used to sequence the 16S and ITS fragments,aiming to compare the differences in species composition,quantity,distribution,and abundance of soil microbial communities among different regions.Concurrently,a comprehensive analysis was conducted by integrating environmental factors such as temperature,precipitation,wind-sand activity,and ecological conditions.The research focused on investigating the composition and diversity distribution characteristics of microbial communities in wolfberry-growing soils in Ningxia,and analyzing the impacts of environmental factors on soil microecological differences,in order to provide references for the healthy development of the wolfberry industry.The results showed that the top 5 fungal abundances in the soils of wolfberry plantations in seven regions were Fusarium、Neocosmospora、Botryotrichum、Mortierella、Fungi_cls_Incertae_sedis,respectively.The top 5 bacterial abundances in the soil of wolfberry in different regions were norank_f__Gemmatimonadaceae、norank_f__Vicinamibacteraceae、norank_o__Vicinamibacterales、norank_f__A4b、MND1,respectively.Diversity analyses showed that three areas,Hongliugou,Zhouta and Xiamaguan,had high similarity in community composition.The community structure of the seven areas showed complementary relationships between fungi and bacteria.The beta diversity of both fungi and bacteria in the soil was significantly different.There were significant differences in soil microbial community composition and diversity among different wolfberry cultivation regions in Ningxia,with fungal and bacterial distributions showing a complementary relationship.By integrating regional factors such as temperature,precipitation,wind-sand conditions,and ecological environment,the study suggests that precipitation may play a crucial role in shaping the microecological variations across different soil regions.
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备注/Memo
第一作者简介：岳苑（1982-），女，博士，高级工程师，现主要从事食品微生物和分子生物学等研究工作。E-mail：yueyuan_0421@126.com.责任作者：龚杰(1985-)，男，博士，副研究员，现主要从事真菌学等研究工作。E-mail：gongjie@icdc.cn.基金项目：宁夏回族自治区重点研发资助项目（20245BEG02027）；宁夏回族自治区市场监管厅科技计划资助项目（2023SJKY0002）。收稿日期：2025-08-04