SUN Xiaoyang,LIU Tongsheng,WU Xuanyang,et al.Identification and Shade Stress Response Analysis of the PIF Gene Family in Bermudagrass[J].Northern Horticulture,2026,(2):27-36.[doi:10.11937/bfyy.20252655]
狗牙根PIF基因家族鉴定及遮荫胁迫分析
- Title:
- Identification and Shade Stress Response Analysis of the PIF Gene Family in Bermudagrass
- 文章编号:
- 1001-0009(2026)02-0027-10
- Keywords:
- bermudagrass; PIF gene; gene family identification; shade stress
- 分类号:
- S668.4
- 文献标志码:
- A
- 摘要:
- 以狗牙根品种‘A12359’为试材，采用生物信息学相关方法，研究了PIF基因亲缘关系、基因结构、保守基序、启动子顺式作用元件以及遮荫胁迫下基因的表达模式，以期揭示该基因家族在遮荫胁迫响应中的潜在作用,为狗牙根的品种改良和分子育种提供参考依据。结果表明：狗牙根中鉴定了33个CdPIFs基因，与其他物种相比，数量较多，可能与其基因组复制历史和生境适应性相关。系统发育分析将33个基因分为4个亚家族。保守基序分析发现，CdPIF基因家族大多数包含motif 1、motif 3和motif 7，显示出高度的保守性。启动子顺式作用元件分析表明，启动子区域富含光响应、激素应答和胁迫响应元件，特别是光响应方面元件的数量最多，强调光在CdPIFs基因调控中的重要性。基因连锁分析与染色体定位结果表明，多个CdPIFs基因在染色体上分布不均。转录组分析表明，相较于其他处理，遮荫6 h处理出现显著性差异表达基因，CdPIF15、CdPIF25、CdPIF26和CdPIF27基因在遮荫胁迫一直显著性高表达。
- Abstract:
- Taking bermudagrass (Cynodon dactylon L.) variety ‘A12359’ as the test material,bioinformatics related methods were used to study the PIF gene relationship,gene structure,conserved motifs,promoter cis-acting elements,and gene expression patterns under shading stress,in order to reveal the potential role of this gene family in shading stress response and provide reference for the improvement of bermudagrass varieties and molecular breeding.The results showed that 33 CdPIFs genes were identified in bermudagrass，a relatively high number compared to other species，which may be attributed to its genome duplication history and habitat adaptation.Phylogenetic analysis classified these 33 genes into four subfamilies.Conserved motif analysis demonstrated that most CdPIFs genes contain motif 1，motif 3，and motif 7，indicating high evolutionary conservation.Promoter cis-acting element analysis revealed an enrichment of light-responsive，hormone-responsive，and stress-responsive elements，with light-responsive elements being the most abundant，underscoring the critical role of light in CdPIFs gene regulation.Gene linkage and chromosomal localization analyses indicated an uneven distribution of multiple CdPIF genes across chromosomes.Transcriptomic analysis identified significant differential gene expression in the 6-hour shade treatment group compared to other treatments.Notably，CdPIF15，CdPIF25，CdPIF26，and CdPIF27 exhibited consistently high expression levels under shade stress.
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备注/Memo
第一作者简介：孙晓阳（1994-），男，博士，讲师，现主要从事草坪草遗传育种等研究工作。E-mail：sunxiaoyang@qau.edu.cn.责任作者：付金民（1961-），男，博士，教授，现主要从事草坪草遗传育种等研究工作。E-mail：turfcn@qq.com.基金项目：国家自然科学基金资助项目（32401481）；山东省重点研发资助项目（2024LZGC00303）。收稿日期：2025-07-20