MENG Xuejiao,FENG Yixin,WANG Lidong,et al.Gene Expression Analysis of Chinese Cabbage After Infection With Pathogen of Black Rot[J].Northern Horticulture,2026,(2):86-93.[doi:10.11937/bfyy.20252606]
大白菜黑腐病菌侵染后基因表达分析
- Title:
- Gene Expression Analysis of Chinese Cabbage After Infection With Pathogen of Black Rot
- 文章编号:
- 1001-0009(2026)02-0086-08
- Keywords:
- Chinese cabbage; disease resistance; black rot; transcriptomics
- 分类号:
- S634.1
- 文献标志码:
- A
- 摘要:
- 以抗黑腐病和感黑腐病2种不同抗性大白菜品种为试材，采用转录组学和生物信息学方法，研究了黑腐病菌侵染不同时间后的基因表达情况，以期为大白菜抗黑腐病新品种培育提供参考依据。结果表明：转录组学共检测到37 228 个表达基因，其中34 752 个已知基因，2 476个新基因；抗病品种3个处理共有差异表达基因997个，感病品种3个处理共有1 593个差异表达基因；6个比较组中共有157个差异表达基因，Pathway富集发现，这些基因主要参与苯丙烷类生物合成途径、植物的昼夜节律、氨基酸代谢等。聚类分析筛选出3个抗病关键基因，在抗病组中表达上调，在感病组中表达下调，基因鉴定为丝氨酸/苏氨酸蛋白激酶（protein-serine/threonine kinase）等。
- Abstract:
- Two different resistant and susceptible Chinese cabbage varieties to black rot were used as test materials.Transcriptomics and bioinformatics methods were used to investigate gene expression at different times after infection by the black rot pathogen，in order to provide reference for the breeding of new varieties of Chinese cabbage resistant to black rot disease.The results showed that a total of 37 228 genes were detected and expressed by transcriptomics，of which 34 752 were known genes and 2 476 new genes were predicted.There were 997 differential genes in the three treatments of the resistant variety and 1 593 differential genes in the three treatments of the susceptible variety.There were 157 differential genes in the six comparison groups.Pathway enrichment analysis revealed that these genes were mainly involved in benzoxazinoid biosynthesis，circadian rhythm-plant，amino acid metabolism，and so on.Cluster analysis screened out three key genes for disease resistance，which were up-regulated in the disease-resistant group and down-regulated in the susceptible group.The genes were identified as protein-serine/threonine kinase，and so on.
参考文献/References:
［1］康宇，王国贵，明佳佳，等．十字花科作物富硒特性及营养品质研究进展［J］．北方园艺，2025(17):123-131．［2］于非.东北地区春白菜高效栽培技术［J］.北方园艺，2024(1):154-156．［3］孟雪娇，陈立新，冯一新，等.东北地区保护地春白菜栽培技术［J］．北方园艺，2023(18):150-151．［4］WILLIAMS P H．Black rot：A continuing［J］．Plant Disease，1980，64(8)：736.［5］李树德.中国主要蔬菜抗病育种进展［M］.北京：科学出版社，1995，583-584.［6］WILLIAMS P H．Inheritance of resistance in cabbage to black rot［J］．Phytopathology，1972，62(2)：247.［7］李明远．十字花科蔬菜黑腐病的发生与防治［J］．当代蔬菜，2004(11)：36-37.［8］李永镐，徐丽波．甘蓝黑腐病苗期抗病性鉴定方法的研究［J］．东北农学院学报，1990，21(2)：125-129.［9］肖崇刚．一种甘蓝黑腐病接种新方法［J］．植物保护，1994，20(5)：35.［10］程伯瑛，武永慧，王翠仙，等．惠丰甘蓝对黑腐病的抗性鉴定研究［J］．北方园艺，2002(6)：48-49.［11］吴国顺．日本对几种蔬菜病害抗病性鉴定方法的研究［J］．中国蔬菜，1994(1)：59-60.［12］何礼远，孙福在，华静月，等．油菜黑腐病病原细菌的鉴定［J］．植物保护学报，1983，10(3)：179-184.［13］张吉昌，邓志勇，司华．油菜黑腐病危害损失测定研究初报［J］．陕西农业科学，1997，43(2)：19-20.［14］张玉勋，曲士松，黄宝勇，等．萝卜种质资源抗黑腐病鉴定［J］．山东农业科学，2000，32(6)：33-34.［15］曲士松，张炎光，张玉勋，等．萝卜优异种质资源的鉴定与评价［J］．黑龙江农业科学，2002(2)：16-18.［16］吴晓丽，李建民，段留生，等．花椰菜幼苗叶片抗氧化酶系统与抗黑腐病关系的研究［J］．植物病理学报，2005，35(6)：509-513.［17］汪恩国，陈克松，李达林．青花菜黑腐病空间分布格局测定及其应用［J］．中国植保导刊，2004，24(2)：30-31.［18］马红梅，朱飞，刘相东．绿菜花黑腐病的发生与防治［J］．植物医生，2003(4)：17-18.［19］李树德．中国主要蔬菜抗病育种进展［M］．北京：科学出版社，1995.［20］DUBROW Z E，BOGDANOVE A J．Genomic insights advance the fight against black rot of crucifers［J］．Journal of General Plant Pathology，2021，87(3)：127-136.［21］吴晓丽，李巧云，解备涛，等．黑腐病菌对抗感不同花椰菜品种叶片超微结构的影响［J］．作物杂志，2010(2)：11-14.［22］张黎黎．青花菜黑腐病抗性遗传及其防御酶活性和超微结构变化的研究［D］．呼和浩特：内蒙古农业大学，2013.［23］GABRIELA R C V，CARMO L S T，SILVA L P，et al.Identification of proteins in susceptible and resistant Brassica oleracea responsive to Xanthomonas campestris pv.campestris infection［J］.Journal of Proteomics，2016，143：278-285.［24］ANDRADE A E，SILVA L P，PEREIRA J L，et al．In vivo proteome analysis of Xanthomonas campestris pv．campestris in the interaction with the host plant Brassica oleracea［J］．FEMS Microbiology Letters，2008，281(2)：167-174.［25］SANTOS C，MAXIMIANO M R，RIBEIRO D G，et al．Differential accumulation of Xanthomonas campestris pv．campestris proteins during the interaction with the host plant：Contributions of an in vivo system［J］．Proteomics，2017，17(12):1700086.［26］朱妍，王超．利用SSH技术分离甘蓝抗黑腐病相关基因的研究［J］．中国蔬菜，2010(10)：20-24.［27］江汉民，郝擘，于雪梅，等．花椰菜抗黑腐病消减cDNA文库的构建和分析［J］．南开大学学报(自然科学版)，2010，43(2)：15-22.［28］吴春珲，李锡香，邱杨，等．萝卜黑腐病抗源受病菌侵染后的基因表达反应［C］//郑州:中国园艺学会2019年学术年会暨成立90周年纪念大会，2019.
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备注/Memo
第一作者简介：孟雪娇（1986-），女，博士，副研究员，现主要从事白菜抗性育种与栽培等研究工作。E-mail:xuejiao0116@126.com.责任作者：冯一新（1972-），男，硕士，高级农艺师，现主要从事集约化育苗及蔬菜栽培等研究工作。E-mail:fyx0451@163.com.基金项目：黑龙江省省属科研院所科研业务费资助项目（CZKYF2023-1-C022）；国家大宗蔬菜产业技术体系哈尔滨综合试验站资助项目（CARS-23-01G）。收稿日期：2025-07-16