WU Xianfeng,GUO Fangyue,CAO Yusong,et al.Effects of Aluminum Stress on Seed Germination and Seedling Growth of Hibiscus sabdariffa[J].Northern Horticulture,2026,(3):24-32.[doi:10.11937/bfyy.20252491]
铝胁迫对玫瑰茄种子萌发及幼苗生长的影响
- Title:
- Effects of Aluminum Stress on Seed Germination and Seedling Growth of Hibiscus sabdariffa
- 文章编号:
- 1001-0009(2026)03-0024-09
- Keywords:
- aluminum stress; Hibiscus sabdariffa; seed germination; growth characteristics; photosynthetic characteristics
- 分类号:
- S567.219
- 文献标志码:
- A
- 摘要:
- 以玫瑰茄种子为试材,通过水培萌发试验与土培幼苗试验,设置200 mg·L-1(T1)、400 mg·L-1(T2)、800 mg·L-1(T3)和1 600 mg·L-1(T4)4个AlCl3处理(pH 4.5),分析测定了铝胁迫下玫瑰茄种子的萌发进程、萌发率、生长指标和玫瑰茄幼苗的相对叶绿素含量及叶绿素荧光参数,研究了不同浓度铝胁迫对玫瑰茄种子萌发、幼苗生长及光合特性的影响,以期为南方红壤区玫瑰茄抗逆栽培及边际土地利用提供参考依据。结果表明:1)铝胁迫显著抑制种子萌发进程,T4处理发芽势(37.6%)与发芽率(39.2%)较CK分别降低48.4%和60.8%(P<0.05),且萌发峰值延迟至第5天。2)幼苗生物量积累受抑制,T4处理鲜质量(0.776 g)较CK(1.908 g)显著下降56.4%。3)根系形态响应敏感,T4处理胚根长(5.50 mm)和直径(1.17 mm)较CK降幅达85.2%和16.6%,但T1处理根直径(1.44 mm)出现适应性增粗。4)光合特性方面,T4处理相对叶绿素含量(SPAD 42.3)显著降低11.2%,光化学淬灭系数(qP=0.727)和电子传递速率(ETR=185.7)较CK下降9.4%和9.2%(P<0.05),而PSⅡ最大光化学效率(Fv/Fm>0.8)未受显著影响。综上,铝胁迫对玫瑰茄种子萌发、幼苗生长及光合作用效率具有显著的抑制作用,高浓度铝胁迫(≥800 mg·L-1)通过抑制胚根伸长、降低光合电子传递效率显著阻碍玫瑰茄早期生长发育,400 mg·L-1可能为其耐受阈值。
- Abstract:
- Taking roselle (Hibiscus sabdariffa L.) seeds as the experimental materials,this study investigated the effects of aluminum (Al) stress on seed germination,seedling growth,and photosynthetic characteristics through hydroponic germination and soil-based seedling culture experiments.Four AlCl3 treatment levels were established,200 mg·L-1 (T1),400 mg·L-1 (T2),800 mg·L-1 (T3),and 1 600 mg·L-1 (T4),all at pH 4.5.Germination dynamics,germination rate,seedling growth parameters,relative chlorophyll content (SPAD value),and chlorophyll fluorescence parameters under Al stress were analyzed,in order to provide reference for stress-resistant cultivation and utilization of marginal lands for roselle in southern red soil regions.The results showed that,1) Al stress significantly inhibited the seed germination process.Under T4 treatment,the germination potential (37.6%) and final germination rate (39.2%) decreased by 48.4% and 60.8%,respectively,compared to CK (P<0.05),and the germination peak was delayed until the 5th day.2) Seedling biomass accumulation was suppressed.The fresh weight under T4 treatment (0.776 g) was significantly reduced by 56.4% compared to CK (1.908 g).3) Root morphology exhibited sensitive responses,radicle length (5.50 mm) and diameter (1.17 mm) under T4 treatment decreased by 85.2% and 16.6%,respectively,compared to CK.However,an adaptive increase in root diameter (1.44 mm) was observed under T1 treatment.4) Regarding photosynthetic traits,the relative chlorophyll content (SPAD 42.3) under T4 treatment decreased significantly by 11.2%.The photochemical quenching coefficient (qP=0.727) and electron transport rate (ETR=185.7) decreased by 9.4% and 9.2%,respectively,compared to CK (P<0.05),whereas the maximum photochemical efficiency of PSⅡ (Fv/Fm>0.8) remained unaffected.In conclusion,Al stress significantly inhibits seed germination,seedling growth,and photosynthetic efficiency in roselle.High concentrations of Al (≥800 mg·L-1) markedly impede early growth and development by suppressing radicle elongation and reducing photosynthetic electron transport efficiency.The tolerance threshold for roselle was suggested to be approximately 400 mg·L-1.
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备注/Memo
第一作者简介:武县丰(1999-),男,硕士研究生,研究方向为生物与医药。E-mail:2696293748@qq.com.责任作者:曹裕松(1978-),男,博士,副教授,现主要从事土壤生态等研究工作。E-mail:15170697218@139.com.基金项目:国家自然科学基金资助项目(31360138);江西省水利科学院科研平台开放基金资助项目(2022SKLS01)。收稿日期:2025-07-08