LIU Zhen,GUO Xuerui,CAO Huaning,et al.Effects of Straw and Microplastics Addition on Soil Fungal Community in Coastal Saline-alkali Area[J].Northern Horticulture,2026,(3):102-110.[doi:10.11937/bfyy.20252429]
秸秆和微塑料添加对滨海盐碱区蔬菜及土壤真菌群落的影响
- Title:
- Effects of Straw and Microplastics Addition on Soil Fungal Community in Coastal Saline-alkali Area
- 文章编号:
- 1001-0009(2026)03-0102-09
- Keywords:
- microplastics; straw; microbial community; high-throughput sequencing
- 分类号:
- S154.3
- 文献标志码:
- A
- 摘要:
- 以“中甘21”甘蓝为试材，设置对照、微塑料（MPS）以及秸秆与微塑料混合施加（SMPS）7个处理，采用Illumina高通量测序对ITS1区进行高通量测序以及FunGuild功能预测，研究微塑料、秸秆施用对土壤真菌微生物群落的影响，以期为滨海盐碱区微塑料污染生态研究提供参考依据。结果表明：MPS2处理（微塑料浓度0.5%）观测到的物种数量、Chaol指数增加，SMPS2处理土壤优势菌门子囊菌门相对丰度提升显著，微生物功能病理营养型相对丰度显著降低。与对照相比，添加微塑料土壤中真菌群落的Shannon指数、Simpson指数均有所降低，Chao1指数在添加微塑料0.5%浓度时有增加趋势，添加微塑料一定程度上影响土壤α多样性。添加秸秆后以上指数均有降低趋势。在土壤中无论是否添加微塑料与以及微塑料与秸秆混施在土壤群落结构方面，真菌群落优势门均为子囊菌门，随着微塑料浓度的增加子囊菌门的相对丰度有增加趋势，加入秸秆后子囊菌门相对丰度显著增加，其中SMPS2子囊菌门相对丰度达94%以上。秸秆的添加为微生物提供了土壤通气、营养物质等环境条件，导致土壤微生物优势属发生变化，腐生营养型微生物相对丰度增加，病理营养型相对丰度减少。
- Abstract:
- Taking ‘Zhonggan 21’ cabbage as the test material,seven treatments including control,microplastics (MPS),and mixed application of straw and microplastics (SMPS) were set up.Illumina high-throughput sequencing was used to perform high-throughput sequencing of ITS1 region and FunGuild functional prediction to study the effects of microplastics and straw application on soil fungal microbial communities,in order to provide reference for ecological research on microplastic pollution in coastal saline alkali areas.The results showed that the number of species and Chaol index observed in MPS2 treatment (microplastic concentration 5%) increased，the relative abundance of soil dominant phylum Ascomycota in SMPS2 treatment increased significantly，and the relative abundance of microbial functional pathological nutrition type decreased significantly.Compared with the control，the Shannon index and Simpson index of the fungal community in the soil added with microplastics decreased，and the Chao1 index increased when the microplastics concentration was 5%.The addition of microplastics affected the alpha diversity of the soil to a certain extent.After adding straw，the above indexes showed a decreasing trend.In terms of soil community structure，the dominant phylum of fungal community was Ascomycota regardless of whether microplastics were added to the soil or not and whether microplastics were mixed with straw.The relative abundance of Ascomycota increased with the increase of microplastics concentration.The relative abundance of Ascomycota increased significantly after the addition of straw.The relative abundance of SMPS2 Ascomycota was more than 94%.The addition of straw provided soil aeration，nutrients and other environmental conditions for microorganisms，resulting in changes in the dominant genera of soil microorganisms.The relative abundance of saprophytic microorganisms increased and the relative abundance of pathological nutrients decreased.
参考文献/References:
［1］赵岩，陈学庚，温浩军，等．农田残膜污染治理技术研究现状与展望［J］．农业机械学报，2017，48(6)：1-14．［2］李娟，王亚静，陈月波，等．聚合物包膜控释肥料膜壳累积、降解及对土壤质量影响的研究进展［J］．植物营养与肥料学报，2022，28(6)：1113-1121．［3］卫洲．微塑料在土壤中的分布及其对有机污染物吸附特征的研究［D］．淮南：安徽理工大学，2022．［4］钱益斌，何小康，杨帅，等．典型热带区域农用地微塑料残留特征及种植类型影响［J］．安全与环境学报，2024，24(11)：4475-4483．［5］王喜英，莫忠妹，李德燕，等．生物炭对微塑料污染下普通白菜生长和土壤细菌群落结构的影响［J］．中国蔬菜，2024(1)：46-59．［6］WANG J，LIU X，LI Y，et al．Microplastics as contaminants in the soil environment：A mini-review［J］．Science of The Total Environment，2019，691：848-857．［7］刘勇，马杰，冯冰聪，等．微塑料迁移及其复合污染对土壤生态系统的影响研究进展［J］．农业环境科学学报，2025，44(3)：537-553．［8］贾瑞峰，丛日环，徐志宇，等．秸秆还田技术改善土壤理化性质提高作物产量的Meta分析［J］．农业工程学报，2025，41(4)：80-89．［9］郑志杰,熊兴军,张秀玲,等.微塑料与秸秆添加对橘园土壤氮磷淋溶的影响［J］.环境科学，2024,45(10):6061-6067.［10］周巧林，汪吉东，尚昊林，等．聚氨酯微塑料和秸秆添加对滨海脱盐潮土有机碳矿化及其组分的影响［J］．生态与农村环境学报，2023，39(8)：1086-1095．［11］秦宇辉，李芬，肖佳敏，等．农田微塑料对水稻秸秆分解及微生物活性的影响［J］．西南农业学报，2022，35(4)：748-756．［12］韦婧，涂晨，杨杰，等．微塑料对农田土壤理化性质、土壤微生物群落结构与功能的影响［J］．生态与农村环境学报，2023，39(5)：644-652．［13］REN X，TANG J，LIU X，et al．Effects of microplastics on greenhouse gas emissions and the microbial community in fertilized soil［J］．Environmental Pollution，2020，256：113347．［14］周东兴，王广栋，邬欣慧，等．不同还田量对秸秆养分释放规律及微生物功能多样性的影响［J］．土壤通报，2018，49(4)：848-855．［15］纪程，孙玉香，孟圆，等．稻麦轮作体系长期秸秆还田对土壤真菌群落结构及秸秆降解潜力的影响［J］．农业环境科学学报，2022，41(4)：819-825．［16］严涵．覆膜栽培下施加生物炭对风沙土中微塑料特性及微生物群落的影响［D］．乌鲁木齐：新疆农业大学，2023．［17］CHALLACOMBE J F，HESSE C N，BRAMER L M，et al．Genomes and secretomes of Ascomycota fungi reveal diverse functions in plant biomass decomposition and pathogenesis［J］．BMC Genomics，2019，20(1)：976．［18］范萍．微塑料对土壤溶解性有机质组成和结构的影响［D］．南昌：南昌大学，2022．［19］余红，史聆聆．微塑料对土壤不同团聚体组分中微生物群落和功能基因的影响［J］．环境工程，2024，42(2)：167-174．［20］LIN Y，YE G，KUZYAKOV Y，et al．Long-term manure application increases soil organic matter and aggregation，and alters microbial community structure and keystone taxa［J］．Soil Biology and Biochemistry，2019，134：187-196．［21］GUO L，ZHENG S，CAO C，et al．Tillage practices and straw-returning methods affect topsoil bacterial community and organic C under a rice-wheat cropping system in Central China［J］．Scientific Reports，2016(6)：33155．［22］GAO R，LIU R，SUN C．A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene［J］．Journal of Hazardous Materials，2022，431：128617．［23］万仁鹏.植物腐生、病原及内生真菌中CAZymes的比较基因组学分析［D］.南昌市:江西师范大学，2019.［24］陈玲玲，张书东，邰思涵，等．‘红阳’猕猴桃软腐病病原菌细极链格孢菌的基因组组装与功能分析［J］．六盘水师范学院学报，2024，36(3)：92-100．［25］侯军华，檀文炳，余红，等．土壤环境中微塑料的污染现状及其影响研究进展［J］．环境工程，2020，38(2)：15,16-27．
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备注/Memo
第一作者简介：刘震（1985-），男，硕士，副研究员，现主要从事作物栽培与施肥等研究工作。E-mail：liuzhen84575151@163.com.基金项目：沧州市自然科学基金资助项目（221001001D）;河北省中央引导地方科技发展资金资助项目（246Z6402G）。收稿日期：2025-07-03