[1]赵英力,张佳琪,吴楠,等.干旱胁迫下外源亚精胺对黄芩种苗抗旱性的生理生化机制[J].北方园艺,2026,(3):52-58.[doi:10.11937/bfyy.20252419]
　ZHAO Yingli,ZHANG Jiaqi,WU Nan,et al.Physiological and Biochemical Mechanisms of Exogenous Spermidine on Drought Resistance of Scutellaria Baicalensis Seedlings Under Drought Stress[J].Northern Horticulture,2026,(3):52-58.[doi:10.11937/bfyy.20252419]
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干旱胁迫下外源亚精胺对黄芩种苗抗旱性的生理生化机制

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年3 页码: 52-58 栏目: 出版日期: 2026-02-15
Title:
Physiological and Biochemical Mechanisms of Exogenous Spermidine on Drought Resistance of Scutellaria Baicalensis Seedlings Under Drought Stress
文章编号:
1001-0009(2026)03-0052-07
作者:
赵英力1; 张佳琪1; 吴楠2; 赵春颖3; 李冬杰4; 刘灵娣2
（1.国家林业和草原局 林产发展规划院,北京 100010;2.河北省农林科学院 经济作物研究所，河北 石家庄 050057;3.承德医学院 中药研究所,河北 承德 067000;4.河北科技大学 食品与生物学院,河北 石家庄 050018）
Author(s):
ZHAO Yingli1; ZHANG Jiaqi1; WU Nan2; ZHAO Chunying3; LI Dongjie4; LIU Lingdi2
(1.Institute of Industrial Development Planning，National Forestry and Grassland Administration，Beijing 100010;2.Institute of Economic Crop Reserch，Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei，050057;3.Institute of Traditional Chinese Medicine，Chengde Medical University,Chengde,Hebei 067000;4.College of Food Science and Biology，Hebei University of Science and Technology，Shijiazhuang，Hebei 050018)
关键词:
干旱胁迫; 亚精胺; 甘肃黄芩; 热河黄芩; 种子萌发; 种苗生理特性
Keywords:
drought stress; Spd; Scutellaria rehderiana; Scutellaria baicalensis; seed germination; seedling physiological characteristics
分类号:
S853.75
DOI:
10.11937/bfyy.20252419
文献标志码:
A
摘要:
以甘肃黄芩（Scutellaria rehderiana）和热河黄芩（Scutellaria baicalensis）为试材，采用不同浓度亚精胺（Spd）浸种，测定了种子的发芽率、发芽指数、活力指数和种苗的根活力、游离脯氨酸含量、渗透势、丙二醛（MDA）含量及细胞膜相对透性，研究了干旱胁迫下外源Spd对黄芩种苗抗旱性影响的生理生化机制，以期为黄芩耐旱品种选育及干旱地区黄芩节水抗旱栽培提供参考依据。结果表明:Spd 处理能显著缓解干旱胁迫对黄芩种子的伤害，促进种子萌发，提高种苗耐旱性。甘肃黄芩以0.50 mmol·L-1和0.75 mmol·L-1 Spd 浸种效果最佳，种子萌发各项指标最高，游离脯氨酸含量、SOD、CAT及POD活性比对照分别提高 88.47%、61.62%、77.87%及82.22% (P＜0.05)，丙二醛含量、膜透性和渗透势比对照分别降低 46.40%、38.76%、81.41% (P＜0.05);热河黄芩以 0.75 mmol·L-1 Spd 浸种效果最佳，〖JP+2〗种子萌发各项指标最高，游离脯氨酸含量、SOD、CAT及POD活性比对照分别提高 85.08%、69.22%、62.29%及79.02% ( P＜0.05)，丙二醛含量、膜透性和渗透势比对照分别降低 35.83%、48.21%、81.28% ( P＜0.05) 。适宜浓度外源 Spd 浸种，可显著提高干旱胁迫下黄芩种苗抗旱性,甘肃黄芩最适Spd浸种浓度为 0.50 mmol·L-1和0.75 mmol·L-1，热河黄芩为 0.75 mmol·L-1 。
Abstract:
Taking Scutellaria rehderiana from Gansu (Scutellaria rehderiana) and Rehe (Scutellaria baicalensis) as the test materials，different concentrations of Spd were used to soak seeds，the germination rate，germination index，vitality index，root vitality，free proline content，osmotic potential，malondialdehyde (MDA) content，and relative membrane permeability of seedlings were measured.The physiological and biochemical mechanisms of exogenous Spd on the drought resistance of Scutellaria baicalensis seedlings under drought stress were studied，in order to provide reference for breading of drought-tolerant varieties of Scutellaria baicalensis and the water-saving and drought-resistant cultivation of Scutellaria baicalensis in arid areas.The results showed that Spd treatment could significantly alleviate the damage of drought stress to Scutellaria baicalensis seeds，promote seed germination，and improve seedling drought tolerance.Gansu Scutellaria baicalensis had the best seed soaking effect with 0.50 mmol·L-1 and 0.75 mmol·L-1 Spd，with the highest seed germination indicators.Compared to the controls，the free proline content，SOD，CAT，and POD activities of the seedlings increased by 88.47%，61.62%，77.87%，and 82.22%,respectively (P＜0.05)，and the malondialdehyde content，membrane permeability，and osmotic potential decreased by 46.40%，38.76%，and 81.41%，respectively (P＜0.05).For Rehe Scutellaria baicalensis，the best soaking effect was achieved with adding 0.75 mmol·L-1 Spd，and all indicators of seed germination were the highest.Compared to the controls，the free proline content，SOD，CAT，and POD activities of the seedlings increased by 85.08%，69.22%，62.29% and 79.02%，respectively (P＜0.05)，while the malondialdehyde content，membrane permeability，and osmotic potential decreased by 35.83%，48.21% and 81.28%，respectively (P＜0.05).Soaking seeds with an appropriate concentration of exogenous Spd could significantly enhance the drought resistance of Scutellaria baicalensis seedlings under drought stress.The optimal soaking concentration of Spd was 0.50 mmol·L-1 and 0.75 mmol·L-1 for Gansu Scutellaria rehderiana，and 0.75 mmol·L-1 for Rehe Scutellaria baicalensis.

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备注/Memo

第一作者简介：赵英力（1987-），男，硕士，高级工程师，现主要从事植物资源开发利用等研究工作。E-mail:hbslyt@163.com.责任作者：刘灵娣（1978-），女，博士，研究员，现主要从事中药材种质资源等研究工作。E-mail:ldj618@163.com.基金项目：国家现代农业产业技术体系建设专项资助项目(CARS-21）；河北省自然科学基金资助项目（H2023301001）；河北省中药材产业技术体系创新团队建设资助项目(HBCT2023080206)；河北省重点研发计划中医药创新专项资助项目(23377501D)；河北省林果花卉产业专家支撑团队资助项目（LC2025-11）。收稿日期：2025-07-03

更新日期/Last Update: 2026-02-24