HU Jiangtao,ZHANG Jingyan,ZHOU Ying,et al.Identification and Biological Characteristics of the Pathogen Indentification Pepper Root Rot and Its Biological Research[J].Northern Horticulture,2026,(2):94-102.[doi:10.11937/bfyy.20252086]
辣椒根腐病病原鉴定、生物学特性及生防研究
- Title:
- Identification and Biological Characteristics of the Pathogen Indentification Pepper Root Rot and Its Biological Research
- 文章编号:
- 1001-0009(2026)02-0094-09
- Keywords:
- Bacillus velezensis; pepper; root rot; pathogen identification; biological characteristics; prevention and control assessment
- 分类号:
- S641.3;S435.6
- 文献标志码:
- A
- 摘要:
- 以崇义县设施辣椒根腐病病害样本为试材，采用组织分离法、形态学与分子生物学鉴定、菌丝抑制法及平板拮抗与温室盆栽防效测定等方法，研究了贝莱斯芽孢杆菌HJT6对辣椒根腐病病原菌GF12的影响，以期为辣椒根腐病的生物防控提供参考依据。结果表明：结合形态学特征并基于ITS、tef-1α和Cbh-c多基因系统发育分析，明确引发赣南地区辣椒根腐病的病原菌GF12为尖孢镰刀菌Fusarium oxysporum。在温度、pH、碳源、氮源分别为25 ℃、8、可溶性淀粉、硝酸钾时，菌丝生长速率最快，分别为6.25、6.22、6.62 mm·d-1和6.22 mm·d-1。B.velezensis HJT6对F.oxysporum GF12具有显著抑制作用，平板拮抗抑制率为58.44%，共培养对菌丝生长的抑制率为36.31%，可刺激分生孢子增加12.5倍，温室盆栽防效为48.35%。综上所述，B.velezensis HJT6对F.oxysporum GF12具有较好的室内抑制效果，可作为拮抗辣椒根腐病的菌种资源。
- Abstract:
- Taking samples of pepper root rot disease in Chongyi county as the test materials,the effects of Bacillus velezensis HJT6 on the pathogen GF12 of pepper root rot disease were studied using methods such as tissue isolation,morphological and molecular biology identification,mycelial inhibition,plate antagonism,and greenhouse pot cultivation efficacy determination,in order to provide reference for the biological control of pepper root rot disease.The results showed that based on morphological characteristics and phylogenetic analysis of ITS,tef-1α and Cbh-c multi gene systems,it was determined that the pathogen GF12 causing pepper root rot in the Gannan area was F.oxysporum.When the temperature,pH,carbon source,and nitrogen source were 25 ℃,8,soluble starch and potassium nitrate,the mycelial growth rate were the fastest,at 6.25 mm·d-1,6.22 mm·d-1,6.62 mm·d-1 and 6.22 mm·d-1,respectively.B.velezensis HJT6 on F.oxysporum GF12 had a significant inhibitory effect,with a plate antagonistic inhibition rate of 58.44% and a co-culture inhibition rate of 36.31% on hyphal growth.It could stimulate 12.5 times increase in conidia,and the greenhouse potted pest control effect was 48.35%.In conclusion,B.velezensis HJT6 a good indoor inhibitory effect on F.oxysporum GF12 and could be used as a bacterial resource for antagonizing pepper root rot.
参考文献/References:
［1］IKEDA K．Role of perithecia as an inoculum source for stem rot type of pepper root rot caused by Fusarium solani f．sp．piperis (teleomorph:Nectria haematococca f．sp．piperis)［J］．Journal of General Plant Pathology,2010,76(4):241-246．［2］徐巧玲,陈世安,陈璐,等．我国辣椒根部病害发生的种类及症状［J］．长江蔬菜,2015(15):51-53,55．［3］刘洋,刘长远,赵奎华,等．辣椒根腐病菌-尖镰孢菌生物学特性研究［J］．沈阳农业大学学报,2009,40(3):291-295．［4］申君,杨绍丽,谷清义,等．一株辣椒根腐病拮抗木霉菌Tb1的筛选与鉴定［J］．东北农业科学,2022,47(5):62-66．［5］李树江,张韵霞,刘羽,等．辣椒根腐病生防菌的筛选鉴定及生防作用［J］．中国蔬菜,2023(9):69-76．［6］CHEN Q,QIU Y,YUAN Y,et al．Biocontrol activity and action mechanism of Bacillus velezensis strain SDTB038 against Fusarium crown and root rot of tomato［J］．Frontiers in Microbiology,2022（13）:994716．［7］李洋.2021年国内新登记的生物农药品种［J］．世界农药,2022,44(2):1-8．［8］谷清义,王妍佳,李梦楠,等．枯草芽孢杆菌BSCY-1对黄瓜种子萌发和幼苗生长特性的影响［J］．北方园艺,2024(10):17-23.［9］曹艳子,顾艳丽,高丽,等．贝莱斯芽孢杆菌的研究现状与应用展望［J］．中国微生态学杂志,2024,36(3):351-356,362．［10］竺利红,李孝辉,施跃峰．芽胞杆菌SM905的鉴定及其对铁皮石斛胶孢炭疽菌的抑菌活性研究［J］．中国生物防治学报,2022,38(2):469-475．［11］LI X J,YAO C X,QIU R,et al．Isolation,identification,and evaluation of the biocontrol potential of a Bacillus velezensis strain against tobacco root rot caused by Fusarium oxysporum［J］．Journal of Applied Microbiology,2023,134(1):lxac049．［12］车建美,赖恭梯,赖呈纯,等．芽孢杆菌FJAT-55034的鉴定、生长特性及对梨轮纹病菌的抑菌活性［J］．微生物学通报,2023,50(11):4925-4937．［13］廖梦婕．芽胞杆菌防治辣椒灰霉病及番茄根结线虫病的机理研究［D］．南京:南京农业大学,2018．［14］胡江涛,刘奇,周英,等．芽孢杆菌hjt6的鉴定、生长特性及其对瓠瓜灰霉病菌的拮抗效果［J］．微生物学通报,2024,51(3):832-845．［15］KVAS M,MARASAS W F O,WINGFIELD B D,et al.Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex［J］.Fungal Diversity,2009,34(2)：1-21.［16］HATSCH D,PHALIP V,JELTSCH J M．Use of genes encoding cellobiohydrolase-C and topoisomerase Ⅱ as targets for phylogenetic analysis and identification of Fusarium［J］．Research in Microbiology,2004,155(4):290-296．［17］蔡高磊,赵昌松,彭宣和,等．十堰辣椒根腐病病原菌分离鉴定及高氏15号的生物防治作用研究［J］．华中师范大学学报(自然科学版),2019,53(2):248-254．［18］KHUDIER N H T,ABDALMOOHSIN R G．Assessment the efficiency of the bioagent Bacillus velesensis and the biohealth formula with fungicide beltanol in control of Fusarium solani causing pepper root rot［J］．IOP Conference Series:Earth and Environmental Science,2023,1262(3):032010．［19］杨明英,曹继芬,李向东,等．辣椒苗期不同生长阶段对疫霉根腐病的抗性研究［J］．西南农业学报,2015,28(1):155-158．［20］ESFAHANI M N,NASEHI A,RAHMANSHIRAZI P,et al．Susceptibility assessment of bell pepper genotypes to crown and root rot disease［J］．Archives of Phytopathology and Plant Protection,2014,47(8):944-953．［21］郝蓉蓉,郭海东,杨成德,等．西藏设施辣椒根腐病病原的分离及鉴定［J］．西北农业学报,2015,24(12):139-143．［22］EARS A A,LANED U B.Disease comment of Capisum Fusarium［J］.Chinese peper,2002(2)：47-48.［23］O′DONNELL K,KISTLER H C,CIGELNIK E,et al．Multiple evolutionary origins of the fungus causing Panama disease of banana:Concordant evidence from nuclear and mitochondrial gene genealogies［J］．Proceedings of the National Academy of Sciences,1998,95(5):2044-2049．［24］连芸芸,李焕宇,李惠霞,等．甘肃省庆阳市辣椒镰孢菌根腐病病原鉴定及生物学特性［J］．甘肃农业大学学报,2021,56(5):55-61,68．［25］张亭亭,刘长远,赵奎华,等．辽宁省辣椒土传病害镰刀菌鉴定及rDNA-ITS序列分析［J］．植物保护,2011,37(5):101-105．［26］陈乐,苗则彦,孙柏欣,等．辽宁省韭菜灰霉病菌的分类鉴定及生物学特性研究［J］．中国蔬菜,2023(5):72-78．［27］崇晓琪,程琳,孙亮,等．辣椒种传病害检测与消毒方法探究［J］．耕作与栽培,2022,42(5):36-41．［28］WADA A C,BDLIYA B S,TURAKI Z G S,et al．Role of seed in disease transmission and the need for effective seed health testing centres in Nigeria［J］．Outlook on Agriculture,2002,31(1):55-62．［29］AL-MASHHADANI S J.The effectiveness of integrated control in controlling some root rot pathogens on Celosia argentea［D］.Karbala:University of Karbala,2022.［30］陈彦,刘长远,王晓红,等．辣椒根腐病化学防治田间试验［J］．植物保护,2008,34(6):150-152．［31］李晓宇．解淀粉芽孢杆菌LXY-6-2筛选、鉴定、诱变与发酵条件优化及其对辣椒根腐病的生物防治研究［D］．杨凌:西北农林科技大学,2018．［32］王振学,党伟,王庆广．生物菌剂对日光温室辣椒根腐病的防效试验［J］．科学种养,2014(4):30-31．［33］张雷．解淀粉芽孢杆菌15-1-1发酵条件优化及对辣椒根腐病防治效果初探［D］．哈尔滨:东北农业大学,2014．
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(2):6.
[3]张婷玉,林 多,杨延杰.辣椒根系分泌物的收集方法研究[J].北方园艺,2014,38(12):14.
ZHANG Ting-yu,LIN Duo,YANG Yan-jie.Study on Collection Method of Root Exudates on Pepper[J].Northern Horticulture,2014,38(2):14.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(2):50.
[5]孔小平,苗增建.等离子体处理辣椒种子对其性状及产量的影响[J].北方园艺,2013,37(13):34.
KONG Xiao-ping,MIAO Zeng-jian.Effect of Plasma Treatment on Characters and Output of Pepper Seeds[J].Northern Horticulture,2013,37(2):34.
[6]高万里.白城地区辣椒产业发展对策研究[J].北方园艺,2014,38(04):159.
GAO Wan-li.Study on the Development Countermeasure of Pepper Industry in Baicheng Area[J].Northern Horticulture,2014,38(2):159.
[7]岳振平.不同种植密度对早春大棚辣椒产量和产值的影响[J].北方园艺,2014,38(05):51.
YUE Zhen-ping.Effect of Different Planting Densty on Fruit Yield and Output Value of Pepper in Early Spring[J].Northern Horticulture,2014,38(2):51.
[8]郭亚华,谢立波,王雪,等.辣椒经地面模拟诱变后的RAPD鉴定[J].北方园艺,2014,38(05):95.
GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(2):95.
[9]杨飞,高艳明,李建设.宁夏设施沙培辣椒引种试验[J].北方园艺,2013,37(10):44.
YANG Fei,GAO Yan-ming,LI Jian-she.Introduction Test of Sand Culture Facilities Pepper in Ningxia[J].Northern Horticulture,2013,37(2):44.
[10]马寿宾,孙艳,王琛,等.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013,37(10):107.
MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(2):107.
备注/Memo
第一作者简介：胡江涛（1993-），男，硕士，农艺师，现主要从事作物病虫害防治等研究工作。E-mail：1303485637@qq.com.责任作者：骆振营（1990-），男，硕士，高级农艺师，现主要从事病虫害防治等研究工作。E-mail：lzhy8115@163.com.基金项目：赣州市重点研发计划资助项目（GZ2024ZDY029，赣市科发［2024］33号，赣市科发［2025］23号）。收稿日期：2025-06-06