GUO Dongming,XI Yanhua,JIA Panwen,et al.Plant Growth-promoting Effect of Bacillus velezensis Strain CBJ-1 on Brassica chinensis[J].Northern Horticulture,2025,(21):27-34.[doi:10.11937/bfyy.20251620]
贝莱斯芽孢杆菌CBJ-1对小油菜的促生效应
- Title:
- Plant Growth-promoting Effect of Bacillus velezensis Strain CBJ-1 on Brassica chinensis
- 文章编号:
- 1001-0009(2025)21-0027-08
- Keywords:
- Bacillus velezensis; biological function; Brassica chinensis L.; seed germination; promoting effect
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以“好润青”小油菜种子为试材，采用催芽和灌根方法，研究了不同稀释倍数发酵液对小油菜种子萌发、幼苗形态以及根际土壤理化指标的影响，评估其在小油菜栽培中的促生效应，以期为小油菜栽培提供参考依据。结果表明：菌株CBJ-1具有固氮作用，对磷酸钙和钾长石有一定的活化能力，可分泌蛋白酶、淀粉酶，同时能够产生吲哚乙酸，最高产量达62.15 mg·L-1；该菌株还具有较强的盐碱和重金属Pb2+耐受性，可在pH 4~8、0%~12%NaCl以及4.0 mmol·L-1的Pb2+条件下正常生长；适宜稀释浓度的菌株CBJ-1发酵液对小油菜种子的萌发、植株生长以及土壤理化性质和速效养分状况均有一定的促进作用。综上，菌株CBJ-1具有养分活化和防病促生能力，且对盐碱及Pb2+有较好耐受性，在小油菜作物防病和生产中具有潜在的开发应用价值。
- Abstract:
- Taking ‘Hao Runqing’ rapeseed seeds as test materials，seed germination and root irrigation methods were used to investigate the effects of different dilutions of fermented liquid on the germination of rapeseed seeds，seedling morphology，and the physicochemical indicators of rhizosphere soil，to evaluate its promoting effects in rapeseed cultivation，in order to provide reference for the cultivation of rapeseed.The results showed that strain CBJ-1 had nitrogen-fixing abilities and a certain activation capacity for calcium phosphate and potassium feldspar.It could secrete protease and amylase while also producing IAA，with a peak yield of 62.15 mg·L-1.This strain also exhibits strong tolerance to saline-alkali and heavy metal Pb2+，allowing it to grow normally under conditions of pH 4 to 8，0% to 12% NaCl，and 4.0 mmol·L-1Pb2+.The suitable dilution concentration of strain CBJ-1′s fermented liquid showed a certain promoting effect on the germination of rapeseed seeds，plant growth，as well as on soil physicochemical properties and readily available nutrient status.In conclusion，strain CBJ-1 had nutrient activation and disease prevention capabilities，as well as good tolerance to salinity and Pb2+，which indicates its potential development and application value in disease prevention and production of rapeseed crops.
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备注/Memo
第一作者简介：郭东明(1995-），男，硕士研究生，研究方向为农业有益微生物的筛选及应用。E-mail:G17717765340@163.com.责任作者：王星(1991-)，男，博士，副教授，现主要从事设施蔬菜高效栽培等研究工作。E-mail:wangxinghebeu.edu.cn.基金项目：河北省科学院重点研发资助项目（25305）。收稿日期：2025-04-30