LIU Hui,ZHAO Siqi,GUO Jin,et al.Effects of Low Temperature，High Light and JA on Anthocyanin Aynthesis in Lycium ruthenicum Murray[J].Northern Horticulture,2025,(21):95-103.[doi:10.11937/bfyy.20251561]
低温、强光和JA对黑果枸杞花青素合成的影响
- Title:
- Effects of Low Temperature，High Light and JA on Anthocyanin Aynthesis in Lycium ruthenicum Murray
- 文章编号:
- 1001-0009(2025)21-0095-09
- 分类号:
- S 567.1
- 文献标志码:
- A
- 摘要:
- 以黑果枸杞无菌组培苗为试材，分析低温、强光和茉莉酸(Jasmonic acid，JA)处理后植株花青素含量及合成通路中调节基因及结构基因的相对表达量，探究了环境因子对黑果枸杞花青素合成的影响及其调控作用,以期为人工调控黑果枸杞花青素高效合成提供参考依据。结果表明：低温、强光和JA处理均能显著增加黑果枸杞植株花青素含量，花青素合成通路中的调节基因LrAN2、LrbHLH112和LrAN11及结构基因LrPAL、LrC4H、Lr4CL、LrCHS、LrCHI、LrF3H、LrF3′5′H、LrDFR、LrANS和LrUFGT的表达量均上升，低温、强光及JA处理后，黑果枸杞植株中LrAN2和LrAN11、LrbHLH112和LrAN11、LrbHLH112和LrAN11分别发挥主要调节作用，促进结构基因LrC4H、LrF3H、LrDFR和LrANS；LrPAL、LrCHS、LrF3H、LrDFR和LrANS；Lr4CL、LrANS和LrUFGT的上调表达，提高花青素含量。
- Abstract:
- Taking Lycium ruthenicum seedlings as the experimental material to analyze the anthocyanin content，modulated and structural genes expression of the biosynthesis pathway induced by low temperature，high light and exogenous jasmonic acid，exploring the mechanism of environmental factors on the anthocyanin biosynthesis in L.ruthenicum Murray,in order to provide reference for the artificial regulation of the efficient synthesis of anthocyanins in Lycium ruthenicum Murray.The results showed that the anthocyanin content were significantly increased induced by low temperature，high light and JA in L.ruthenicum Murray seedlings.In the anthocyanin biosynthesis pathway，the expression levels of modulated genes LrAN2,LrbHLH112，and LrAN11，as well as structural genes LrPAL,LrC4H,Lr4CL,LrCHS,LrCHI,LrF3H,LrF3′5′ H,LrDFR,LrANS，and LrUFGT were all upregulated.After low temperature，high light，and JA treatment，LrAN2 and LrAN11,LrbHLH112 and LrAN11,LrbHLH112 and LrAN11 played key regulatory roles separately，promoted the expression of structural genes LrC4H,LrF3H,LrDFR and LrANS；LrPAL,LrCHS,LrF3H,LrDFR and LrANS；Lr4CL,LrANS，and LrUFGT，thereby increased the anthocyanin content.
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备注/Memo
第一作者简介：刘慧（2000-），女，硕士研究生，研究方向为植物次生代谢产物合成与调控。E-mail：3319802894@qq.com.责任作者：王静（1984-），女，博士，副教授，现主要从事植物次生代谢产物合成与调控等研究工作。E-mail：wangjing_imu2@163.com.基金项目：国家自然科学基金资助项目（32160392）；宁夏回族自治区重点研发计划资助项目（2021BEF02002）。收稿日期：2025-04-26