TAN Siqi,HUANG Jiajia,ZENG Yuyu,et al.Study on the Molecular Mechanism of Sawdust Degradability Discrepancy in Stropharia rugosoannulata Strains[J].Northern Horticulture,2025,(21):16-26.[doi:10.11937/bfyy.20251333]
大球盖菇菌株木屑降解能力差异的分子机制研究
- Title:
- Study on the Molecular Mechanism of Sawdust Degradability Discrepancy in Stropharia rugosoannulata Strains
- 文章编号:
- 1001-0009(2025)21-0016-11
- Keywords:
- Stropharia rugosoannulata; lignin degradation; enzyme activity assays; transcriptome analysis; molecular mechanism
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以降解能力强大球盖菇菌株SA44和降解能力弱菌株SA07为试材，采用酶活测定和转录组分析方法，研究其木屑降解能力差异的分子机制，以期为选育出高产木质素降解酶的菌株提供参考依据。结果表明：SA44菌株的漆酶和过氧化物酶活性显著高于SA07菌株，这与其较高的木屑降解能力一致。在2个菌株间，差异表达基因主要富集在碳水化合物活性酶家族，涉及氧化酶活性、糖苷水解酶功能及解毒蛋白编码。SA44菌株中与木质素修饰和降解相关的辅助酶、氧化酶、糖苷水解酶以及解毒蛋白的基因表达水平显著上调，这些基因的协同作用可能增强了木屑降解效率。综上，关键酶基因表达的差异、辅助酶系统的协同作用以及胞外酶系统的组成与活性差异，是大球盖菇菌株木屑降解能力存在差异的原因，进一步构建了相关的分子机制模型。
- Abstract:
- Taking the strong degradability strain SA44 and the poor one SA07 of Stropharia rugosoannulata as the test materials，enzyme activity determination and transcriptome analysis were used to investigate the molecular mechanisms underlying the differences in their sawdust degradation abilities，in order to provide reference for the selection of S.rugosoannulata strains with high-yield lignin degrading enzyme.The results showed that the laccase and peroxidase activities of the strain SA44 were significantly higher than those of SA07，consistent with its enhanced sawdust degradability.Between the two strains，the differentially expressed genes were predominantly enriched in the carbohydrate-active enzyme families，which mainly encode genes involved in oxidase activities，glycoside hydrolase functions，and detoxification.In the strain SA44，the expression levels of genes related to lignin modification and degradation，including those encoding auxiliary enzymes，oxidases，glycoside hydrolases，and detoxification proteins，were significantly upregulated.The synergistic action of these genes likely enhanced the degradation efficiency of sawdust.In summary，the differences in the expression of key enzyme genes，the synergistic effect of auxiliary enzyme systems，and the differences in the composition and activity of extracellular enzyme systems were the reasons for the differences in sawdust degradability discrepancy between the two S.rugosoannulata strains.Further，a relevant molecular mechanism model was constructed here.
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备注/Memo
第一作者简介：谭思琪（2001-），女，硕士研究生，研究方向为食用菌组学。E-mail：tansiqi@webmail.hzau.edu.cn.责任作者：肖扬（1979-），男，博士，教授，现主要从事食用真菌学等研究工作。E-mail：xiaoyang@mail.hzau.edu.cn.基金项目：国家食用菌产业技术体系资助项目（CARS-20）；湖北省食用菌产业技术体系资助项目（2023HBSTX4-09）。收稿日期：2025-04-09