[1]张天静,孙文松,刘坤,等.苍术根腐病病原菌鉴定、生物学特性及对杀菌剂敏感性分析[J].北方园艺,2025,(19):102-109.[doi:10.11937/bfyy.20251076]
　ZHANG Tianjing,SUN Wensong,LIU Kun,et al.Analysis on Identification，Biological Characteristics and Fungicide Sensitivity of the Pathogen Causing Atractylodes lancea Root Rot[J].Northern Horticulture,2025,(19):102-109.[doi:10.11937/bfyy.20251076]
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苍术根腐病病原菌鉴定、生物学特性及对杀菌剂敏感性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年19 页码: 102-109 栏目: 出版日期: 2025-10-15
Title:
Analysis on Identification，Biological Characteristics and Fungicide Sensitivity of the Pathogen Causing Atractylodes lancea Root Rot
文章编号:
1001-0009(2025)19-0102-08
作者:
张天静1; 2; 孙文松2; 3; 刘坤1; 2; 王卫星3
（1.辽宁省经济作物研究所，辽宁 辽阳 111000；2.辽宁省农业科学院 药用植物研究所，辽宁 辽阳 111000；3.辽宁清新环境科技有限公司，辽宁 辽阳 111000）
Author(s):
ZHANG Tianjing1; 2; SUN Wensong2; 3; LIU Kun1; 2; WANG Weixing3
(1.Liaoning Research Institute of Cash Crops,Liaoyang，Liaoning 111000;2.Institute of Medicinal Plants,Liaoning Academy of Agricultural Sciences,Liaoyang，Liaoning 111000;3.Liaoning Qingxin Environmental Technology Co.Ltd.,Liaoyang,Liaoning 111000)
关键词:
苍术; 根腐病; 尖孢镰孢菌; 生物学特性; 杀菌剂; 敏感性
Keywords:
Atractylodes lancea; root rot;  Fusarium oxysporum; biological characteristics; fungicide; sensitivity
分类号:
S 435.672
DOI:
10.11937/bfyy.20251076
文献标志码:
A
摘要:
以辽宁阜蒙县具有根腐病症状的苍术病株为试材，采用组织分离法对病原菌进行分离；根据科赫氏法则对分离菌株进行致病性测定，通过形态学和分子生物学鉴定病原菌种类，并研究其生物学特性；采用菌丝生长速率法测定了病原菌对11种杀菌剂的敏感性，以期为苍术根腐病病害有效防治提供参考依据。结果表明：苍术根腐病主要致病菌为尖孢镰孢菌Fusarium oxysporum，其最适培养基为Richard和察氏培养基，最适氮源为硝酸钾和硝酸钠，最适碳源为蔗糖、甘露醇和麦芽糖。室内药剂试验结果显示，已唑·四霉素和福·霜·敌磺钠对苍术根腐病病原菌抑制效果最好，EC50值<1 mg·L-1，其次是甲霜·噁霉灵。
Abstract:
Taking Atractylodes lancea plants with root rot from Fumeng country,Liaoning Province as experimental materials,the pathogen was isolated and purified by using tissue isolation method.Based on Koch′s Postulates,the pathogenicity of the isolated strains was determined.The pathogen species were identified through morphological and molecular biological methods,and their biological characteristics were studied.The sensitivity of the pathogen to 11 fungicides was determined using the mycelial growth rate method,in order to provide reference for the effective prevention and control of root rot disease in Atractylodes lancea.The results showed that the pathogen causing Atractylodes lancea root rot was Fusarium oxysporum.The biological characteristics of the pathogen showed that the optimal media was Richard and Czapek，the optimal nitrogen were potassium nitrate and sodium nitrate，the carbon were sucrose，mannitol and maltose.Indoor toxicity tests against pathogen showed that pyrazole tetracycline and fenaminosulf had a better inhibitory effect on mycelial growth，with EC50 value<1 mg·L-1，the second was Metalaxyl·Hymexazol.

参考文献/References:

［1］TSUSAKA T，MAKINO B，OHSAWA R，et al．Genetic and environmental factors influencing the contents of essential oil compounds in Atractylodes lancea［J］．PLoS One，2019，14(5)：e0217522．［2］杨璐平，盖聪，周梦琪，等．麻黄-苍术治疗新型冠状病毒肺炎机制的网络药理学探讨［J］．中药材，2020，43(7)：1779-1785．［3］王铁霖，郭兰萍，张燕，等．苍术常见病害的病原、发病规律及综合防治［J］．中国中药杂志，2016，41(13)：2411-2415.［4］李阔，王升，王红阳，等．苍术根际橘绿木霉的生防功能及培养条件的探索［J］．中国实验方剂学杂志，2024，30(15)：192-199.［5］郭兰萍，黄璐琦，蒋有绪，等．栽培苍术根际土壤微生物变化［J］．中国中药杂志，2007，32(12)：1131-1133．［6］高鑫媛，赵红玲，白茹玥，等．北苍术根腐病植株根际土壤真菌群落多样性分析［J］．西南农业学报，2022，35(4)：812-821.［7］ZHOU X，GUO Z，CHEN C，et al．Soil microbial community structure and diversity are largely influenced by soil pH and nutrient quality in 78-year-old tree plantations［J］．Biogeosciences，2017，14(8)：2101-2111．［8］ROUSK J，BROOKES P C，BTH E．Investigating the mechanisms for the opposing pH relationships of fungal and bacterial growth in soil［J］．Soil Biology and Biochemistry，2010，42(6)：926-934．［9］霍佳欢，温晓蕾，李双民，等．北苍术根腐病病原鉴定及生物学特性研究［J］．中国农业科技导报，2022，24(5)：137-144．［10］黄俊斌，李建洪，王沫，等．茅苍术主要病害的发生特点及其综合防治技术初控［C］.西安：全国第六届天然药物资源学术研讨会，2004.［11］唐贵婷，蒋欢，苏宇，等．重庆南苍术根腐病病原鉴定［J］．植物病理学报，2021，51(4)：641-645．［12］曹敏，余米，唐祥友，等．茅苍术根腐病主要病原菌的分离鉴定及室内药剂筛选［J］．中药材，2022，45(8)：1789-1795．［13］徐可可，刘永红，马静婷，等．一株地黄根腐病致病菌的鉴定及生物学特性研究［J］．中国中药杂志，2024，49(18)：4898-4904．［14］方中达．植病研究方法［M］.3版．北京：中国农业出版社，1998.［15］HE C Y，WOLYN D J．Potential role for salicylic acid in induced resistance of Asparagus roots to Fusarium oxysporum f．sp．asparagi［J］．Plant Pathology，2005，54(2)：227-232．［16］LESLIE J F，SUMMERELL B A．The Fusarium Laboratory Manual［M］．New York：John Wiley & Sons，2006．［17］魏景超遗．真菌鉴定手册［M］．上海：上海科学技术出版社，1979．［18］COLLINS C H，LYNE P M，GRANGE J M,et al.Collins & Lyne′s microbiological methods［M］.London:Oxford University Press Inc,2004.［19］沈晋良．农药生物测定［M］．北京：中国农业出版社，2013．［20］沈丹琦，周晓琪，许雪松，等．蓝莓枝枯病病原菌鉴定、生物学特性及对杀菌剂敏感性研究［J］．北方园艺，2024(5)：39-47.［21］穆向荣，马逾英，杨枝中，等．药用植物根腐病防治的研究进展［J］．中药与临床，2014，5(2)：5-8，52．［22］张天静,孙文松,沈宝宇,等.辽藁本根腐病病原菌鉴定及生物学特性［J］.北方园艺,2023(14):118-124.［23］高芬，任小霞，王梦亮，等．中草药根腐病及其微生物防治研究进展［J］．中国中药杂志，2015，40(21)：4122-4126．［24］沈清清，刘芳，胡彦．药用植物根腐病病原菌研究进展［J］．北方园艺，2014(11)：187-190.

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备注/Memo

第一作者简介：张天静（1979-），女，硕士，助理研究员，现主要从事中药材病害综合防治等研究工作。E-mail：873613102@qq.com.责任作者：孙文松（1979-），男，硕士，研究员，现主要从事中药材种质收集保护与品种选育及标准化栽培等研究工作。E-mail:sunwensong12@126.com.基金项目：国家中药材产业技术体系建设专项资金资助项目（CARS-21-06）；国家重点研发计划资助项目（2024YFD1600602）；辽宁省农业科学院学科建设计划资助项目（2019DD144221）。收稿日期：2025-03-24

更新日期/Last Update: 2025-10-31