LIU Yufei,PANG Dandan,CHEN Chunlin,et al.Cloning and Expression Analysis of the Coding Region of CsSTK in Tea Plants[J].Northern Horticulture,2025,(21):1-7.[doi:10.11937/bfyy.20251038]
茶树CsSTK编码区的克隆与表达分析
- Title:
- Cloning and Expression Analysis of the Coding Region of CsSTK in Tea Plants
- 文章编号:
- 1001-0009(2025)21-0001-07
- Keywords:
- tea plants; CsSTK; gene cloning; expression analysis; flower development
- 分类号:
- S 571.1
- 文献标志码:
- A
- 摘要:
- 以茶树‘云茶1号’和“云茶奇蕊”为试材，采用RT-PCR技术克隆获得CsSTK基因的编码区序列，并对其进行生物信息学分析及表达模式研究，以期为深入了解CsSTK调控茶树生殖发育的分子机制提供参考依据。结果表明：CsSTK基因开放阅读框长696 bp，编码231个氨基酸，编码蛋白含有MADS-box和K-box结构域，属于MADS-box家族；系统进化分析显示，CsSTK与其他植物STK蛋白高度同源，尤其与山茶花CjSTK蛋白亲缘关系最近。组织特异性表达分析表明，CsSTK在茶树花和果实中高表达，而在雌蕊缺失种质‘云茶奇蕊’花中表达量显著降低，表明其可能参与茶树花和果实，尤其与雌蕊的发育可能更为密切；蛋白互作分析显示，CsSTK可能通过与SEP3、AGL80、BEL1等多个MADS-box蛋白互作参与花发育调控，并受泛素化修饰影响。
- Abstract:
- Taking the tea plant cultivar ‘Yuncha 1’ and ‘Yuncha Qirui’ as experimental materials，the coding sequence of the CsSTK was cloned via RT-PCR technique，followed by bioinformatic analysis and expression pattern investigation，in order to provide reference for a deeper understanding of the molecular mechanisms by which CsSTK regulates reproductive development in tea plants.The results showed that the CsSTK gene had an open reading frame of 696 bp，encoding 231 amino acids.The encoded protein contains MADS-box and K-box domains，classifying it within the MADS-box family.Phylogenetic analysis revealed that CsSTK was highly homologous to STK proteins from other plants，with the closest relationship to the CjSTK protein of Camellia japonica.Tissue-specific expression analysis showed that CsSTK was highly expressed in the flowers and fruits of the tea plant，while its expression was significantly reduced in the flowers of the gynoecium-deficient germplasm ‘Yuncha Qirui’，suggested its potential involvement in the development of flowers and fruits，particularly closely related to gynoecium development.Protein interaction analysis indicated that CsSTK may participate in the regulation of flower development through interactions with several MADS-box proteins such as SEP3，AGL80，and BEL1，and was influenced by ubiquitination modifications.
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备注/Memo
第一作者简介：刘玉飞(1992-)，男，硕士，助理研究员，现主要从事茶树遗传改良等研究工作。E-mail：chaye18@163.com.责任作者：陈林波（1980-），男，硕士，研究员，现主要从事茶树育种与遗传改良等研究工作。E-mail：chenlinbo2002@sina.com.基金项目：云南省基础研究计划资助项目（202201AT070060，202401AT070088）；云南省农业基础研究联合专项资助项目（202101BD070001-035，202301BD070001-081）；国家自然科学基金资助项目（32260784，32060699）。收稿日期：2025-03-20