[1]宋茁,黄瑶,范海燕,等.沙棘枯萎病生防菌株GT52-2的鉴定与发酵条件优化[J].北方园艺,2025,(19):51-60.[doi:10.11937/bfyy.20251026]
　SONG Zhuo,HUANG Yao,FAN Haiyan,et al.Identification of Biocontrol Strain GT52-2 and Optimization of Fermentation Conditions for Stem Wilt of Sea Buckthorn[J].Northern Horticulture,2025,(19):51-60.[doi:10.11937/bfyy.20251026]

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沙棘枯萎病生防菌株GT52-2的鉴定与发酵条件优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年19 页码: 51-60 栏目: 出版日期: 2025-10-15

Title:: Identification of Biocontrol Strain GT52-2 and Optimization of Fermentation Conditions for Stem Wilt of Sea Buckthorn

文章编号:: 1001-0009(2025)19-0051-10

作者:: 宋茁1; 黄瑶2; 范海燕1; 梁月3; 韩娅茹1; 夏博2; （1.沈阳农业大学植物保护学院，辽宁沈阳 110866；2.贵州大学昆虫研究所，贵州贵阳 550025；3.水利部沙棘开发管理中心，北京 100083）

Author(s):: SONG Zhuo1; HUANG Yao2; FAN Haiyan1; LIANG Yue3; HAN Yaru1; XIA Bo2; (1.College of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.Institute of Entomology,Guizhou University,Guiyang,Guizhou 550025;3.Sea-buckthorn Development and Management Center,Ministry of Water Resources,Beijing 100038)

关键词:: 沙棘枯萎病; 拟枝孢镰刀菌; 生物防治; 芽孢杆菌; 发酵条件优化

Keywords:: sea buckthorn wilt disease; Fusarium sporotrichioides; biological control; Bacillus; fermentation condition optimization

分类号:: S 793.6

DOI:: 10.11937/bfyy.20251026

文献标志码:: A

摘要:: 以甘肃省庆阳市沙棘种植园沙棘根际土壤为试材，采用稀释涂布平板法、平板对峙法和菌丝生长速率法进行生防细菌的分离筛选，获得了对沙棘枯萎病病原菌拟枝孢镰刀菌F.sporotrichioides具有较好抑制作用的生防菌株GT52-2；通过形态学、生理生化特征、分子生物学方法对筛选出的生防菌株进行鉴定，并测定抑菌谱；通过单因素试验筛选菌株最佳发酵条件，研究培养条件对菌株抑菌活性的影响，以期为生防菌株的高效利用和沙棘枯萎病的生物防控提供参考依据。结果表明：生防菌株GT52-2为解淀粉芽孢杆菌Bacillus amyloliquefaciens，抑菌率为65.12%；菌株具有较广的抑菌谱；菌株GT52-2培养优化条件为麦芽糖10 g·L-1、丙氨酸10 g·L-1、发酵时间48 h、pH 8、装液量20 mL、转速200 r·min-1。

Abstract:: Taking rhizosphere soil of sea buckthorn from sea buckthorn plantations in Qingyang city，Gansu Province as the test material,the dilution plating method，plate confrontation method and mycelial growth rate method were employed for the isolation and screening of biocontrol bacteria，resulting in the obtainment of biocontrol strain GT52-2，which exhibits good inhibitory effect against Fusarium sporotrichioides（the pathogen causing sea buckthorn wilt disease）.The screened biocontrol strain was identified through morphological，physiological and biochemical characteristics as well as molecular biology methods，and its antibacterial spectrum was determined.The optimal fermentation conditions of the strain were screened via single-factor experiments，and the impact of culture conditions on the antibacterial activity of the strain was studied，in order to provide a reference for the efficient utilization of biocontrol strains and the biological control of sea buckthorn wilt disease.The results showed that biological strain GT52-2 was identified as Bacillus amyloliquefaciens，with an inhibition rate of 65.12%.The strain had a broad-spectrum antimicrobial activity.The optimized culture conditions for strain GT52-2 were as follows,maltose 10 g·L-1，alanine 10 g·L-1，fermentation time of 48 hours，pH 8，liquid volume of 20 mL，and a rotation speed of 200 r·min-1.

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备注/Memo

第一作者简介：宋茁（2002-），女，硕士研究生，研究方向为植物病理。E-mail：songzhuo12@stu.syau.edu.cn.责任作者：夏博（1981-），男，博士，副教授，现主要从事植物病理等研究工作。E-mail：xiabo0522@syu.edu.cn.基金项目：新疆生产建设兵团重点领域科技攻关资助项目（2025AB016）；水利部沙棘开发管理中心沙棘病虫害研究专项资助项目（2020-zg-zx-017，2021-zg-kj-013，2022-zg-kj-011，2023-zg-kj-021）；新疆生产建设兵团重点领域科技攻关资助项目（2025AB016）。收稿日期：2025-03-19

更新日期/Last Update: 2025-10-31