YAO Yuyi,CAO Guofan,MA Zhiqiang,et al.Effects of Root Rot Disease on the Microbial Community Structure and Enzyme Activity in the Rhizosphere Soil of Atractylodes macrocephala[J].Northern Horticulture,2025,(19):87-93.[doi:10.11937/bfyy.20250833]
根腐病对白术根际土壤微生物群落结构及酶活性的影响
- Title:
- Effects of Root Rot Disease on the Microbial Community Structure and Enzyme Activity in the Rhizosphere Soil of Atractylodes macrocephala
- 文章编号:
- 1001-0009(2025)19-0087-07
- 分类号:
- S 567.23+3
- 文献标志码:
- A
- 摘要:
- 以白术为试材，采用Illumina HiSeq高通量测序技术对白术根腐病病株和健株的根际土壤微生物多样性进行测序，并对土壤养分及酶活性进行分析，研究了根腐病对其根际土壤微生物多样性、土壤理化性质及酶活性的影响，以期为白术根腐病的防治提供参考依据。结果表明：相较于健株，罹病白术根际土壤细菌群落Alpha多样性显著降低，土壤有机质含量显著增加，速效钾含量显著降低，土壤脲酶、过氧化氢酶显著降低；在门水平上，变形菌门(Proteobacteria)和子囊菌门(Ascomycota)分别作为白术根际土壤细菌和真菌群落的绝对优势菌群存在，其中病株Proteobacteria的相对丰度增加，Ascomycota的相对丰度减少；在属水平上，病株中致病菌群镰刀菌属(Fusarium)的相对丰度增加。
- Abstract:
- Taking Atractylodes macrocephala as the test material，Illumina HiSeq high-throughput sequencing technology was emplyed to analyze the microbial diversity in rhizosphere soil of healthy and root rot-diseased plants.Soil nutrient content and enzyme activities were also examined to investigate the effects of root rot disease on rhizosphere soil microbial diversity，physicochemical properties，and enzyme activities，in order to provide references for the prevention and control of A.macrocephala root rot.The results showed that compared with healthy plants，the Alpha diversity of bacterial communities in diseased A.macrocephala rhizosphere soil was significantly reduced，soil organic matter content increased significantly，while available potassium content decreased significantly in diseased plants，soil urease and catalase activities were significantly lower in diseased plants.At the phylum level，Proteobacteria and Ascomycota were the dominant bacterial and fungal groups，respectively，in A.macrocephala rhizosphere soil.The relative abundance of Proteobacteria increased in diseased plants，while that of Ascomycota decreased.At the genus level，the relative abundance of the pathogenic fungus Fusarium increased in diseased plants.
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备注/Memo
第一作者简介：姚雨宜（1999-），女，硕士研究生，研究方向为药用植物栽培与利用。E-mail:1772085363@qq.com.责任作者：曹国璠（1965-），男，博士，教授，现主要从事作物栽培学教学与科研等工作。E-mail:cgf8933@126.com.基金项目：国家自然科学基金资助项目（3196150373）。收稿日期：2025-03-09