WANG Xinqian,ZENG Wenyue,WANG Jiahui,et al.Effects of Different Light Intensities on Chlorophyll Fluorescence Imaging Characteristics in Leaves of Tomato During Recovery Period After Low Night Temperature[J].Northern Horticulture,2025,(19):15-22.[doi:10.11937/bfyy.20250735]
恢复期不同光强对低夜温后番茄叶片叶绿素荧光成像特性的影响
- Title:
- Effects of Different Light Intensities on Chlorophyll Fluorescence Imaging Characteristics in Leaves of Tomato During Recovery Period After Low Night Temperature
- 文章编号:
- 1001-0009(2025)19-0015-08
- Keywords:
- Solanum lycopersicum L.; low night temperature; light intensity; PSⅡ function; photoinhibition; chlorophyll fluorescence imaging
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以番茄品种“中蔬4号”（Solanum lycopersicum L.cv ‘Zhongshu No.4’）为试材，采用人工模拟低夜温的试验方法，对番茄幼苗进行5 ℃低夜温处理12 h，然后28 ℃常温1 200 μmol·m-2·s-1强光或100 μmol·m-2·s-1弱光恢复4 h，并在处理各阶段进行了叶绿素荧光成像测定，并分析了低夜温后不同光强恢复对番茄叶绿素荧光成像特性的影响，以期揭示光强在低夜温胁迫后番茄PSⅡ功能恢复中的作用，并为番茄冬春设施栽培提供参考依据。结果表明：低夜温处理明显降低了番茄叶片qP和Y(Ⅱ)，但并未引起番茄叶片Fv/Fm和NPQ的变化。与其他荧光参数相比，低夜温及恢复期强光处理导致全叶中qP=0的面积比例增加，这意味着番茄叶片可通过主动关闭PSⅡ反应中心以减少对光能的吸收。恢复期强光导致Fv/Fm和NPQ的持续下降，qP和Y(Ⅱ)也维持在较低水平，而弱光处理则促进了qP、Y(Ⅱ)、NPQ和Y(NPQ)快速恢复至低夜温处理前水平。综上所述，低夜温抑制了番茄叶片PSⅡ功能，导致次日强光下发生光抑制的潜在风险；恢复期强光进一步抑制了番茄叶片PSⅡ功能和热耗散途径，从而加剧了光抑制程度，而弱光处理则有利于PSⅡ功能的恢复。
- Abstract:
- Taking tomato variety (Solanum lycopersicum L.cv ‘Zhongshu No.4’) as test materials，an artificially simulated low night temperature treatment was employed.Tomato seedlings were treated at low night temperature of 5 ℃ (LNT) for 12 hours，and then recovered at 28 ℃ normal temperature under high light (RHL，approximately 1 200 μmol·m-2·s-1) or low light (RLL，approximately 100 μmol·m-2·s-1) for 4 hours.Chlorophyll fluorescence imaging was measured at different stages of the treatments，and the effects of different light intensities on the chlorophyll fluorescence imaging characteristics in leaves of tomato during recovery period after low night temperature were analyzed，in order to reveal the role of light intensity in the recovery of tomato PSⅡ function after low night temperature stress and provide a reference for the winter and spring protected cultivation of tomatoes.The results showed that LNT treatment significantly reduced qP and Y(Ⅱ)，but had no influence on Fv/Fm and NPQ of tomato leaves.Compared with other chlorophyll fluorescence parameters，the proportion of whole leaves with qP=0 increased significantly for tomato leaves under LNT and RHL，which meant that tomato leaves could close the PSⅡ reaction center to reduce the absorption of light energy.Fv/Fm and NPQ continuously declined，and qP and Y(Ⅱ) also remained at a low level in leaves treated under RHL.However，RLL promoted the rapid recovery of qP,Y(Ⅱ),NPQ，and Y(NPQ) to the level of tomato leaves before low night temperature treatment.In conclusion，low night temperature inhibited the PSⅡ function of tomato leaves，leading to a potential risk of photoinhibition under high light the next day.High light during the recovery period further inhibited the PSⅡ function and heat dissipation pathway，thereby exacerbating the degree of photoinhibition.However，low light treatment was beneficial to the recovery of PSⅡ function during the recovery period.
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备注/Memo
第一作者简介：王欣倩（1998-），女，硕士研究生，研究方向为生物与医药。E-mail：2590234324@qq.com.责任作者：胡文海（1973-），男，博士，教授，硕士生导师，现主要从事园艺植物栽培生理生态等研究工作。E-mail：huwenhai@jgsu.edu.cn.基金项目：国家自然科学基金资助项目(32460752)；井冈山大学校级大学生创新创业训练计划资助项目(JDX2024177)。收稿日期：2025-03-03