ZHANG Xiaoyi,JIN Weibo.Research Status and Future Development of SIGS in the Control of Botrytis cinerea[J].Northern Horticulture,2025,(21):141-147.[doi:10.11937/bfyy.20250693]
SIGS在灰霉病防治中的研究现状及未来发展
- Title:
- Research Status and Future Development of SIGS in the Control of Botrytis cinerea
- 文章编号:
- 1001-0009(2025)21-0141-07
- Keywords:
- Botrytis cinerea; dsRNA; SIGS
- 分类号:
- S 62
- 文献标志码:
- A
- 摘要:
- 灰霉病菌（Botrytis cinerea）是一种广泛危害农作物的植物病原真菌，能够侵染多种作物，如番茄、草莓、葡萄和辣椒等，导致严重的经济损失。传统的化学防治方法存在环境污染、抗药性等问题，因此开发绿色、高效的防治技术成为研究热点。近年来，喷雾诱导基因沉默（SIGS）技术作为一种新型的RNA干扰（RNAi）应用手段，因其无需基因改造、操作简便而受到广泛关注。SIGS技术通过喷洒双链RNA（dsRNA）诱导病原菌基因沉默，从而抑制其生长和致病能力。尽管SIGS技术在灰霉病菌的防治研究中展现出显著的防治效果，但仍存在一些不足，如dsRNA的稳定性不足、递送效率较低、脱靶效应的风险以及成本与可持续性问题。该综述总结了SIGS技术在灰霉病防治中的国内外研究现状，包括靶基因的选择、dsRNA的设计与优化、纳米载体的应用，以及面临的挑战和未来发展方向。
- Abstract:
- Botrytis cinerea is a plant pathogenic fungus that widely affects crops，infecting a variety of plants such as tomatoes，strawberries，grapes，and peppers，leading to significant economic losses.Traditional chemical control methods pose issues such as environmental pollution and resistance，thus developing green and efficient control technologies has become a research focus.In recent years，spray-induced gene silencing (SIGS) technology，as a novel RNA interference (RNAi) application method，has gained widespread attention due to its non-requirement for genetic modification and simple operation.SIGS works by spraying double-stranded RNA (dsRNA) to induce gene silencing in pathogens，thereby inhibiting their growth and pathogenicity.Although SIGS has shown significant control effects in research on Botrytis cinerea，it still faces several challenges，such as insufficient stability of dsRNA，low delivery efficiency，risks of off-target effects，and issues regarding cost and sustainability.This review summarized the current state of research on SIGS technology for controlling Botrytis cinerea both domestically and internationally，included target gene selection，dsRNA design and optimization，application of nano-carriers，as well as the challenges and future directions.
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备注/Memo
第一作者简介：张小艺（1998-），女，硕士研究生，研究方向为植物分子生物学。E-mail:zhangxy09222@163.com.责任作者：金伟波（1977-），男，博士，教授，现主要从事植物分子生物学等研究工作。E-mail:jwb@zstu.edu.cn.基金项目：国家自然科学基金资助项目（32172496）。收稿日期：2025-02-28