ZHANG Yanjun,XU Pan,CHEN Hongwei,et al.Effects of Arbuscular mycorrhizal Fungi on the Growth，Absorption and Accumulation of Cadmium in Houttuynia cordata[J].Northern Horticulture,2025,(19):37-44.[doi:10.11937/bfyy.20250651]
丛枝菌根真菌对鱼腥草生长及吸收积累镉的影响
- Title:
- Effects of Arbuscular mycorrhizal Fungi on the Growth，Absorption and Accumulation of Cadmium in Houttuynia cordata
- 文章编号:
- 1001-0009(2025)19-0037-08
- Keywords:
- Houttuynia cordata; Arbuscular mycorrhizal Fungi; absorption and accumulation heavy metals; cadmium
- 分类号:
- S 154.3
- 文献标志码:
- A
- 摘要:
- 以鱼腥草为试材，采用控制变量法设置3个不同的接菌处理，分别为不接种菌根组（non mycorrhiza，NM）、接种摩西斗管囊霉（Funneliformis mosseae，Fm）、接种根内根孢霉（Rhiaophagus intraradices，Ri）；在温室盆栽培养条件下，人为设置50 mg·kg-1镉（Cd）污染胁迫，研究了不同接菌处理的鱼腥草在镉胁迫下与丛枝菌根真菌（Arbuscular mycorrhizal Fungi，AMF）的共生效果、生理状态、重金属Cd的吸收积累量以及抗氧化酶活性，以期为AMF-鱼腥草联合修复技术的深入研究和应用提供参考依据。结果表明：2种AMF菌剂在高浓度Cd胁迫下均能与鱼腥草稳定共生；2种AMF菌剂均可显著提高鱼腥草的叶绿素含量和生物量，促生效应Fm>Ri>NM，但Fm和Ri之间无显著差异；2种AMF均在不同程度上提高了鱼腥草叶片、地上茎及须根中的Cd含量，Cd生物富集系数大小排序为Ri>Fm>NM，Ri较Fm显著提高了鱼腥草Cd吸收量。鱼腥草叶片抗氧化酶及丙二醛（MDA）检测结果表明，接种AMF使MDA含量增加不显著，接种AMF后超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性水平显著升高。综上，AMF（Fm & Ri）有利于鱼腥草在Cd胁迫下生长和吸收积累Cd，AMF可通过提高抗氧化酶活性缓解高Cd积累对鱼腥草造成的损伤，Ri的接种效果更优。
- Abstract:
- Taking Houttuynia cordata as experimental material,three distinct mycorrhizal inoculation treatments were established using the control variable method,the non-mycorrhizal group (non mycorrhiza，NM)，the Funneliformis mosseae (Fm) inoculation group，and the Rhizophagus intraradices (Ri) inoculation group.Under controlled greenhouse pot culture conditions，a cadmium (Cd) pollution stress of 50 mg·kg-1 was artificially introduced to investigate the symbiotic performance，physiological status，Cd uptake and accumulation，as well as antioxidant enzyme activity of Houttuynia cordata across the different mycorrhizal inoculation groups under Cd stress,in order to provide reference for the in-depth research and application of AMF-Houttuynia cordata combined remediation technology.The results showed that both AMF inoculants formed stable symbiotic associations with Houttuynia cordata under high Cd stress.Both Fm and Ri significantly enhanced the chlorophyll content and biomass of Houttuynia cordata，with the growth-promoting effect ranked as Fm>Ri>NM，although no significant difference was observed between Fm and Ri.Both AMF treatments increased the Cd concentration in the leaves，stems，and fibrous roots of Houttuynia cordata to varying degrees.The Cd bioaccumulation coefficient followed the order Ri>Fm>NM，with Ri showing a significantly higher Cd uptake compared to Fm.Analysis of antioxidant enzymes and malondialdehyde (MDA) levels in Houttuynia cordata leaves revealed that AMF inoculation did not significantly elevate MDA content but significantly enhanced the activity of SOD，POD，and CAT.In conclusion，AMF (Fm and Ri) promotes the growth，Cd uptake，and accumulation in Houttuynia cordata under Cd stress.AMF mitigates the adverse effects of high Cd accumulation by enhancing antioxidant enzyme activity，with Ri demonstrating a superior inoculation effect.
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备注/Memo
第一作者简介：张雁君（1995-），女，硕士，研究实习员，现主要从事菌根生物学等研究工作。E-mail:2190627807@qq.com.责任作者：高鹏（1982-），女，硕士，研究员，现主要从事环境污染治理等研究工作。E-mail:ppenggao@.163.com.基金项目：四川省省级科研院所基本科研业务资助项目（2024JDKY0003）。收稿日期：2025-02-06