LIU Dawei,ZHANG Ziru,ZHAO Juan,et al.Screening,Identification and Evaluation of Growth-promoting Effect of Biocontrol Bacteria Against Watermelon Root-knot Nematode Disease[J].Northern Horticulture,2025,(19):80-87.[doi:10.11937/bfyy.20250625]
西瓜根结线虫病生防细菌的筛选鉴定及促生效果评价
- Title:
- Screening,Identification and Evaluation of Growth-promoting Effect of Biocontrol Bacteria Against Watermelon Root-knot Nematode Disease
- 文章编号:
- 1001-0009(2025)19-0080-08
- 分类号:
- S 436.5
- 文献标志码:
- A
- 摘要:
- 以施加过黑曲霉Y61菌剂和哈茨木霉菌剂的西瓜根际土壤为试验样本,采用土壤稀释分离法获得杀南方根结线虫的生防细菌,研究了不同生防细菌对南方根结线虫2龄幼虫的致死率和对植物促生指标的影响,以期筛选出具有良好应用开发前景的菌株。结果表明:粘质沙雷氏菌(Serratia marcescens)对南方根结线虫2龄幼虫的致死率达到100%,盆栽防效为77%,对西瓜种子的发芽具有明显促进作用,能够利用葡萄糖、蔗糖、木糖、甘露醇、麦芽糖、山梨醇等,显示出良好的应用开发前景。
- Abstract:
- Taking the rhizosphere soil of watermelon treated with Aspergillus niger Y61 and Trichoderma harzianum as the experimental samples,the biocontrol bacteria that kill Meloidogyne incognita were obtained by soil dilution and separation method.The mortality rate of different biocontrol bacteria on the second-stage juvenile of M.incognita and their effects on the plant growth promotion indicators were studied,with the aim of screening out strains with good application and development prospects.The results showed that the mortality rate of Serratia marcescens against the second-stage juvenile of M.incognita reached 100%,the pot control effect was 77%,and it had a significant promoting effect on the germination of watermelon seeds.It could utilize glucose,sucrose,xylose,mannitol,maltose,sorbitol,etc.,this strain showed a good prospect of application development.
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备注/Memo
第一作者简介:刘大伟(1983-),男,博士,副教授,现主要从事植物病害生物防治等研究工作。E-mail:liudawei353@163.com.责任作者:刘霆(1975-),男,博士,副研究员,现主要从事植物线虫病害绿色防控等研究工作。E-mail:lting11@163.com.基金项目:北京市农林科学院科技创新资助项目(KJCX20240316,KJCX20230115)。收稿日期:2025-02-25