[1]丁美云,张巍,徐宜彬,等.不同低温环境对蓝靛果忍冬抗寒性生理代谢的影响[J].北方园艺,2025,(12):59-65.[doi:10.11937/bfyy.20250191]
　DING Meiyun,ZHANG Wei,XU Yibin,et al.Effects of Different Low Temperature Environments on the Cold Resistance Physiological Metabolism of Lonicera caerulea[J].Northern Horticulture,2025,(12):59-65.[doi:10.11937/bfyy.20250191]
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不同低温环境对蓝靛果忍冬抗寒性生理代谢的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年12 页码: 59-65 栏目: 出版日期: 2025-06-30
Title:
Effects of Different Low Temperature Environments on the Cold Resistance Physiological Metabolism of Lonicera caerulea
文章编号:
1001-0009(2025)12-0059-07
作者:
丁美云; 张巍; 徐宜彬; 李阳; 沈琦
（黑龙江省林业科学院 伊春分院，黑龙江 伊春 153000）
Author(s):
DING Meiyun; ZHANG Wei; XU Yibin; LI Yang; SHEN Qi
(Yichun Branch,Heilongjiang Academy of Forestry Sciences，Yichun，Heilongjiang 153000)
关键词:
蓝靛果忍冬; 低温胁迫; 抗氧化活性; 膜质损伤; 渗透调节; 生理代谢
Keywords:
Lonicera caerulea; cold stress; antioxidant activity; membrane damage; osmotic adjustment; physiological metabolism
分类号:
S 663
DOI:
10.11937/bfyy.20250191
文献标志码:
A
摘要:
以７个不同品种蓝靛果忍冬的一年生枝条为试材，采用低温胁迫4、-15、-20、-25、-30、-35、-40 ℃方法，研究了不同蓝靛果忍冬品种抗寒性对其抗氧化活性、膜损伤指标和渗透调节能力的影响，以期为低温调控蓝靛果忍冬抗寒性的研究提供参考依据。结果表明：各试验品种的抗寒性随着温度的变化而变化，超氧化物歧化酶（SOD）和过氧化物酶（POD）活性在-20~4 ℃时较高，并在-35 ℃时SOD和POD活性呈现上升的趋势。所有品种SOD和POD活性的变化趋势基本一致。相对电导率在-25～-15 ℃变化较小，MDA含量在-25～-20 ℃变化较小。可溶性糖含量在4 ℃时含量达到最高，随着温度的降低，可溶性糖含量呈现逐渐下降的趋势，但在温度达到-35 ℃时可溶性糖含量又出现明显上升。可溶性蛋白质含量在4 ℃时含量最高，随着温度的降低可溶性蛋白质含量呈现下降趋势，但在-20 ℃和-35 ℃时出现上升趋势。综上，不同品种蓝靛果忍冬抗寒能力‘蓓蕾’＞Y4＞‘乌蓝’＞‘蓝精灵’＞Y2＞Y3＞Y1。
Abstract:
Taking one-year-old branches of seven different varieties of Lonicera caerulea as experimental materials，the effects of cold stress at 4 ℃,-15 ℃,-20 ℃,-25 ℃,-30 ℃,-35 ℃，and -40 ℃ on the antioxidant activity，membrane damage indicators，and osmotic adjustment ability of different Lonicera caerulea varieties were studied,in order to provide a reference for the research on the regulation of cold resistance in Lonicera caerulea by low temperature.The results showed that,the cold resistance of each experimental variety changed with temperature，and the activities of SOD and POD were higher between -20 ℃ and 4 ℃，and showed an increasing trend at -35 ℃.The trend of SOD and POD activity changes were basically consistent among all varieties.The relative conductivity changed little between -25 ℃ and -15 ℃，and the MDA content changed little between -25 ℃ and -20 ℃.The soluble sugar content reached its maximum at 4 ℃，and gradually decreased with decreasing temperature.However，when the temperature reached -35 ℃，the soluble sugar content showed a significant increase again.The soluble protein content was the highest at 4 ℃，and showed a decreasing trend with decreasing temperature.However，there was an increasing trend at -35 ℃ and -20 ℃.In a comprehensive comparison, the cold resistance of different varieties of Lonicera caerulea was ranked as follows ‘Beilei’>Y4>‘Wulan’>‘Lanjingling’>Y2>Y3>Y1.

参考文献/References:

［1］刘彬昕,李晔男,关欣,等.蓝靛果忍冬产业发展的现状、问题及建议［J］.北方果树,2024(4):53-55.［2］HUO J W,YANG G H,SUI W,et al.Review of study on germ plasm resources of blue honeysuckle (Lonicera caerulea L.)(in Chinese)［J］.Journal of Horticulture,2005,32(1):159-164.［3］张富玮,张东亚,李建贵．低温胁迫对蓝靛果忍冬渗透调节物质的影响［J］．经济林研究,2017,35(2):127-131.［4］柴振宇,苏鑫,焦璇,等．响应面法优化蓝靛果无糖酸乳生产工艺及稳定性分析［J］．食品科技,2024,49(6):99-107.［5］AMARARATHNA M,HOSKIND W,VASANTHA R．Cyanidin-3-O-glucoside-rich haskap berry administration suppresses carcinogen-induced lung tumorigenesis in A/JCr mice［J］．Molecules,2020,25(17):3823.［6］GUO L,QIAO J,ZHANG L,et al．Critical review on anthocyanins in blue honeysuckle (Lonicera caerulea L．) and their function［J］．Plant Physiology and Biochemistry,2023,204:108090.［7］李忠贤,喻好好,李亚男,等.咖啡响应低温胁迫的研究进展［J］.热带农业科学,2024,44(10):148-154.［8］张昭．软枣猕猴桃抗寒性研究［D］．哈尔滨:东北农业大学,2019.［9］张志良,瞿伟菁,李小方．植物生理学实验指导［M］.4版．北京:高等教育出版社,2009.［10］李玲．植物生理学模块实验指导［M］．北京:科学出版社,2009.［11］王学奎．植物生理生化实验原理和技术［M］.2版．北京:高等教育出版社,2006.［12］许英,陈建华,朱爱国,等．低温胁迫下植物响应机理的研究进展［J］．中国麻业科学,2015,37(1):40-49.［13］CHINNUSAMY V,ZHU J,ZHU J K．Cold stress regulation of gene expression in plants［J］．Trends in Plant Science,2007,12(10):444-451.［14］徐卫平,蒋景龙,任绪明,等．低温胁迫对3种柑橘幼苗细胞膜及渗透调节的影响［J］．分子植物育种,2017,15(3):1104-1108.［15］孙世航．猕猴桃抗寒性评价体系的建立与应用［D］．北京:中国农业科学院,2018.［16］侯雷平,李梅兰．油菜素内酯(BR)促进植物生长机理研究进展［J］．植物学通报,2001,36(5):560-566.［17］徐松华．逆境条件下植物体内活性氧代谢研究进展［J］．安徽农学通报,2021,27(21):29-32.［18］ISHIKAWA M,ROBERTSON A J,GUSTA L V．Comparison of viability tests for assessing cross-adaptation to freezing,heat and salt stresses induced by abscisic acid in bromegrass (Bromus inermis Leyss) suspension cultured cells［J］．Plant Science,1995,107(1):83-93.［19］艾琳．鲜食葡萄抗寒性研究［D］．乌鲁木齐:新疆农业大学,2003.［20］LIANG Y,CHEN Q I N,LIU Q,et al．Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L．)［J］．Journal of Plant Physiology,2003,160(10):1157-1164.［21］张萱,徐萍,于梦雯,等.低温驯化对灯盏花幼苗抗寒性的影响［J］.北方园艺,2025(5):82-89.［22］JONES M M．Osmotic adjustment in leaves of Sorghum in response to water deficits［J］．Plant Physiology,1978,61(1):122-126.［23］齐安国,刘传冠,吕东阳,等.4个种源无患子幼苗对低温胁迫的生理响应及抗寒性评价［J］.中南林业科技大学学报,2024,44(10):46-54.［24］张小贝,祝志欣,南文卓,等.2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响［J］.安徽农业大学学报,2017,44(3):525-529.［25］SZABADOS L,SAVOURA．Proline:A multifunctional amino acid［J］．Trends in Plant Science,2010,15(2):89-97.

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备注/Memo

第一作者简介：丁美云（1998-），女，硕士，研究实习员，现主要从事森林特产和小浆果等研究工作。E-mail：1844527240@qq.com.责任作者：张巍（1978-），男，硕士，高级工程师,现主要从事森林特产及浆果资源等研究工作。E-mail：Zw81025259@163.com.基金项目：国家重点研发计划资助项目（2022YFD1600502-18）；黑龙江省省属科研院所科研业务费资助项目（CZKYF2024-F09）。收稿日期：2025-01-17

更新日期/Last Update: 2025-07-02