LIU Kun,LU Yilong,LIU Ying,et al.Identification of GRAS Gene Family Members in Panax ginseng and Expression Pattern Analysis[J].Northern Horticulture,2025,(15):21-33.[doi:10.11937/bfyy.20250099]
人参GRAS基因家族成员鉴定及表达模式分析
- Title:
- Identification of GRAS Gene Family Members in Panax ginseng and Expression Pattern Analysis
- 文章编号:
- 1001-0009(2025)15-0021-13
- Keywords:
- Panax ginseng; GRAS gene family; bioinformatics; transcriptome; gene expression
- 分类号:
- S 567.5+1
- 文献标志码:
- A
- 摘要:
- 以人参（Panax ginseng C.A.Meyer）为试材，采用基因组学和比较基因组学分析方法，鉴定和分析了人参GRAS基因家族成员，并对其蛋白理化性质、系统进化关系、基因结构、细胞亚定位和染色体分布情况以及启动子顺式作用元件等进行预测，同时，对该基因家族在人参不同器官和不同生长年限的表达模式进行初步解析，以期为改善人工栽培人参的品质、破解连作障碍、推动人参栽培产业的可持续发展提供参考依据。结果表明：人参基因组中共鉴定到GRAS转录因子基因家族成员119个，不均匀地分布在20条染色体上，分别重命名为Pgras 01~Pgras 119，蛋白序列大小349~799 aa，分子量38.94~87.12 kD。亚细胞定位结果显示，所有成员均位于细胞核。系统发育分析将其划分为13个亚家族，且同一亚家族成员具有相似的基因结构和保守基序。共线性分析发现，家族成员间存在11个串联重复事件，片段重复区域内存在113个GRAS同源基因对，推测片段重复可能是人参GRAS基因变异的主要途径。启动子顺式作用元件分析结果显示，人参GRAS家族成员中光响应元件最多，共有1 409个，其次为植物激素茉莉酸甲酯响应元件，共有248个。此外，人参GRAS基因的表达模式具有器官特异性和生长年限累积效应。
- Abstract:
- Taking Panax ginseng C.A.Meyer as the test material，genomic and comparative genomics analysis methods were used to identify and analyze the members of the ginseng GRAS gene family.The protein physicochemical properties，systematic evolutionary relationships，gene structure，cell sub localization，chromosome distribution，and promoter cis acting elements were predicted.At the same time，the expression patterns of this gene family in different organs and growth years of ginseng were preliminarily analyzed，in order to provide reference for improving the quality of artificially cultivated ginseng，breaking down continuous cropping barriers，and promoting the sustainable development of ginseng cultivation industry.The results showed that a total of 119 GRAS family members were identified from the P.ginseng genome，with uneven distribution across 22 chromosomes，designated as Pgras 1-Pgras 119.The molecular weights of the proteins ranged from 38.94 kD to 87.12 kD，while their protein lengths span from 349 aa to 799 aa.Subcellular localization results showed that all members were in the nucleus.The GRAS proteins were divided into 13 subfamilies by phylogenetic tree analysis，and members of the same subfamily had similar gene structures and conserved Motifs.Collinearity analysis showed that there were 11 tandem repeat events among the family members，and 113 GRAS homologous gene pairs existed in the fragment repeat regions.It was speculated that segmental duplication might be the main pathway of GRAS genes variation in ginseng.The results of promoter cis-acting element analysis showed that the GRAS family members had the highest number of light response elements with 1 409，followed by phytohormone methyl jasmonate response elements with 248.Furthermore，the expression pattern of ginseng GRAS genes had organ specificity and cumulative effect.
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备注/Memo
第一作者简介：刘坤（1986-），男，硕士，助理研究员，现主要从事药用植物病害综合防治等研究工作。E-mail：yuanteng1986@126.com.责任作者：孙文松（1973-），男，硕士，研究员，现主要从事中药材育种与栽培等研究工作。E-mail：sunwensong12@126.com.基金项目：国家中药材产业技术体系辽阳综合试验站资助项目（CARS-21-06）；辽宁省农业科学院学科建设资助项目（2019DD144221）；辽宁省应用基础研究计划（联合计划）资助项目（2022JH2/101300284）；辽宁省农业科学院协同创新“揭榜挂帅”专项资助项目（2022XTCX0503）。收稿日期：2025-01-09