PENG Yunsong,REN Hao,LIU Ting,et al.Effects of High Temperature Stress on the Physiological Characteristics of Edible Lilies[J].Northern Horticulture,2025,(12):66-73.[doi:10.11937/bfyy.20250076]
高温胁迫对食用百合生理特性的影响
- Title:
- Effects of High Temperature Stress on the Physiological Characteristics of Edible Lilies
- 文章编号:
- 1001-0009(2025)12-0066-08
- Keywords:
- high temperature stress; edible lily; physiological response
- 分类号:
- S 682.29
- 文献标志码:
- A
- 摘要:
- 以食用百合品种‘Lily 03’为试材，采用组织培养方法，研究了不同温度梯度（30、35 ℃和40 ℃）及处理时长（24、48 h和72 h）对其生理生化响应机制，以期为提升食用百合的耐热性提供参考依据。结果表明：随温度升高与胁迫时间延长，叶绿素含量持续下降，其中35 ℃处理48 h下降了70.4%；可溶性蛋白质与可溶性糖呈先升后降趋势，35 ℃处理48 h二者分别达峰值；游离脯氨酸含量显著降低，40 ℃处理72 h较对照（CK）下降了82.4%；丙二醛（MDA）含量呈增长趋势，40 ℃处理72 h为对照（CK）的2.13倍；超氧化物歧化酶（SOD）与过氧化物酶（POD）活性呈先增后降趋势，35 ℃处理48 h时活性最高。因此，35 ℃是维持植株正常代谢的临界温度，40 ℃胁迫超过48 h将引发细胞膜系统不可逆损伤，植株通过激活抗氧化酶系统和调控渗透调节物质共同应对高温胁迫，其中游离脯氨酸代谢与叶绿素稳定性是评价耐热性的关键指标。
- Abstract:
- Taking the edible lily variety ‘Lily 03’ as the test material and employing tissue culture methods to investigate the effects of different temperature gradients (30,35 ℃,and 40 ℃) and treatment durations (24,48 hours,and 72 hours) to high temperature stress on the physiological and biochemical response mechanisms,in order to provide reference for the enhancement of the heat tolerance of edible lilies.The results showed that with the increase of temperature and the prolongation of stress time,chlorophyll content continued to decline,of which 35 ℃ treatment 48 hours decreased by 70.4%;soluble protein and soluble sugar showed a trend of first rise and then decline,35 ℃ treatment 48 hours reached the peak,respectively;free proline content was significantly reduced,40 ℃ treatment 72 hours compared with the control (CK) decreased by 82.4%;MDA content showed an increasing trend,40 ℃ MDA content showed an increasing trend,40 ℃ treatment 72 hours for the control (CK) 2.13 times;SOD and POD activity showed a trend of first increase and then decrease,the activity is the highest under 35 ℃ treatment 48 hours.The study showed that 35 ℃ was the critical temperature to maintain the normal metabolism of the plant,40 ℃ stress over 48 hours will trigger irreversible damage to the cell membrane system,the plant through the activation of antioxidant enzyme systems and osmoregulation substances to cope with high temperature stress,of which the free proline metabolism and the stability of chlorophyll are the key indicators for evaluating the heat tolerance.
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备注/Memo
第一作者简介：彭云松(1998-)，男，硕士研究生，研究方向为观赏花卉栽培技术。E-mail：493296100@qq.com.责任作者：徐永艳（1976-），女，博士，副教授，现主要从事花卉栽培技术等研究工作。E-mail:xyy@163.com.基金项目：云南省高校服务重点产业科技资助项目（FWCY-ZNT2024021）。收稿日期：2025-01-08