WANG Weixu,LIU Qibao,LI Tinggang,et al.Identification of WAK/WAKL Gene Family in Grape and Expression Analysis Under White Rot Stress[J].Northern Horticulture,2025,(12):8-18.[doi:10.11937/bfyy.20245003]
葡萄WAK/WAKL基因家族鉴定及在白腐病胁迫下的表达分析
- Title:
- Identification of WAK/WAKL Gene Family in Grape and Expression Analysis Under White Rot Stress
- 文章编号:
- S 663.1
- 关键词:
- 葡萄; VvWAK/WAKL基因家族; 白腐病; 表达模式分析
- Keywords:
- grape; VvWAK/WAKL gene family; white rot; expression pattern
- 分类号:
- 1001-0009(2025)12-0008-11
- 文献标志码:
- A
- 摘要:
- 以葡萄为试材，基于已公布的葡萄基因组序列和注释信息，采用生物信息学方法对葡萄VvWAK/WAKL基因家族进行鉴定，研究了其染色体定位、理化性质、亚细胞定位、二级结构、系统进化、共线性、保守基序、基因结构、启动子顺式作用元件以及在白腐病病原菌侵染下的表达模式，以期为葡萄的遗传改良和抗逆育种提供参考依据。结果表明：葡萄VvWAK基因21个，VvWAKL基因26个，47个VvWAK/WAKL基因分布在10条染色体上，且10、17、18号染色体上数量最多。葡萄VvWAK/WAKL家族蛋白的氨基酸数量在353~1 048 aa，分子量和等电点分别在40.57~116.76 kD和8.07~8.87。葡萄VvWAK/WAKL基因家族亚细胞定位主要在质膜和液泡膜，主要的二级结构是α-螺旋和无规则卷曲。系统发育分析将VvWAK/WAKL基因家族划分为5组，各组间成员保守基序及基因结构相似。进化分析表明VvWAK/WAKL基因家族在葡萄内有22个串联重复基因，与拟南芥存在7对同源基因。在VvWAK/WAKL基因家族的启动子区域检测到大量与光响应有关的元件以及与脱落酸、水杨酸、赤霉素、茉莉酸甲酯、生长素等激素有关的响应元件。在白腐菌株侵染葡萄的转录组数据分析中发现，有18个家族成员在侵染6 h后被高度诱导表达。
- Abstract:
- Taking grape as material,based on the published grape genome sequence and annotation information，the grape VvWAK/WAKL gene family was identified by bioinformatics methods.The chromosome localization，physicochemical properties，subcellular localization，secondary structure，phylogenetic evolution，collinearity，conserved motifs，gene structure，promoter cis-acting elements and expression pattern under the infection of white rot pathogen were studied，in order to provide reference for genetic improvement and stress resistance breeding of grape.The results showed that there were 21 VvWAK genes，26 VvWAKL genes，and 47 VvWAK/WAKL genes were distributed on 10 chromosomes，with the largest number on chromosomes 10,17，and 18.The number of amino acids of VvWAK/WAKL family proteins ranged from 353 aa to 1 048 aa，the molecular weight and isoelectric point ranged from 40.57 kD to 116.76 kD and 8.07 to 8.87，respectively.The subcellular localization of VvWAK/WAKL gene family in Vitis vinifera was mainly in plasma membrane and tonoplast，and the main secondary structures were α-helix and random coil.Phylogenetic analysis divided the VvWAK/WAKL gene family into five groups，and the conserved motifs and gene structures of members in each group were similar.Phylogenetic analysis showed that the VvWAK/WAKL gene family had 22 tandem repeat genes in grapes and 7 pairs of homologous genes with Arabidopsis thaliana.A large number of light-responsive elements and hormone-responsive elements related to abscisic acid，salicylic acid，gibberellin，methyl jasmonate，and auxin were detected in the promoter region of the VvWAK/WAKL gene family.In the transcriptome data analysis of grapes infected by white rot strains，it was found that 18 family members were highly induced after 6 hours of infection.
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备注/Memo
第一作者简介：王伟旭（1999-），男，硕士研究生，研究方向为设施园艺病虫害综合防控。E-mail:879081945@qq.com.责任作者：李楠洋（1986-），男，博士，讲师，现主要从事植物抗病分子机理等研究工作。E-mail：linanyang130430@126.com.基金项目：山东省农业科学院农业科技创新工程资助项目(CXGC2024F16)。收稿日期：2024-12-18