ZHANG Youwei,ZHAO Le,WEN Hao,et al.Physiological Response of Exogenous Melatonin to Seed Germination and Seedling Growth of Bitter Bean Seedling Under Salt Stress[J].Northern Horticulture,2025,(11):56-66.[doi:10.11937/bfyy.20244845]
盐胁迫下外源褪黑素对苦豆子种子萌发及幼苗生长的影响
- Title:
- Physiological Response of Exogenous Melatonin to Seed Germination and Seedling Growth of Bitter Bean Seedling Under Salt Stress
- 文章编号:
- 1001-0009(2025)11-0056-11
- Keywords:
- Sophora alopecuroides; melatonin; NaCl stress; seed germination; physiological characteristics
- 分类号:
- S 853.75
- 文献标志码:
- A
- 摘要:
- 以苦豆子（Sophora alopecuroides）为试材，采用种子浸泡的方法，研究了不同浓度外源MT(50、100、150、200、250 μmol·L-1)对盐胁迫下种子萌发及幼苗生长的影响，以期为苦豆子在盐胁迫逆境条件下的种植和改良土壤理化性质提供参考依据。结果表明：盐胁迫下（T0）苦豆子的种子萌发和幼苗生长发育明显受到抑制作用（P<0.05），与CK相比发芽率、发芽势、发芽指数、活力指数分别降低了32.08%、43.90%、46.93%、59.82%；幼苗株高、根长、叶长、鲜质量、干质量、根系活力分别降低了26.35%、11.52%、13.76%、27.30%、18.64%、53.78%；过氧化物酶(POD)和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性以及相对叶绿素（SPAD）含量受到不同程度的抑制。以上指标在T0~T5处理组中，均呈现先增加后降低的趋势。而T0处理下的丙二醛(MDA)含量、相对电导率以及休眠率与CK相比显著增加（P<0.05），在T0~T5处理组中，呈现先降低后增加的趋势。测定的各项数据运用相关性分析、聚类分析、主成分分析和隶属函数综合评价分析，发现100 μmol·L-1的MT效果最为显著（P<0.05），50 μmol·L-1的MT次之，200 μmol·L-1的MT最差。
- Abstract:
- Taking Sophora alopecuroides as the test material，the effects of different concentrations of exogenous MT (50，100，150，200，250 μmol·L-1) on seed germination and seedling growth under salt stress were studied by soaking the seeds，in order to provide a reference for the cultivation and improvement of soil physicochemical properties of bitter pea under salt stress adversity conditions.The results showed that under salt stress (T0)，the seed germination and seedling growth and development of Sophora alopecuroides were significantly inhibited (P<0.05).Compared with CK，the germination rate，germination potential，germination index，and vitality index decreased by 32.08%，43.90%，46.93%，and 59.82%，respectively.The height，root length，leaf length，fresh weight，dry weight，and root vitality of the seedlings decreased by 26.35%，11.52%，13.76%，27.30%，18.64%，and 53.78%，respectively；The activities of peroxidase (POD)，catalase (CAT)，superoxide dismutase (SOD)，and relative chlorophyll (SPAD) are inhibited to varying degrees.All the above indexes showed a tendency of increasing and then decreasing in the five groups of treatments from T0-T5.While malondialdehyde (MDA) content，relative conductivity，and dormancy rate were significantly increased (P<0.05) under T0 treatment compared with CK，they showed a trend of decreasing and then increasing in the five groups of treatments from T0-T5.The comprehensive evaluation and analysis of the measured data using correlation analysis，cluster analysis，principal component analysis and affiliation function showed that the effect of 100 μmol·L-1 MT was the most significant (P<0.05)，followed by 50 μmol·L-1MT，and 200 μmol·L-1MT was the worst.
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备注/Memo
第一作者简介：张有为（1998-），男，硕士研究生，研究方向为园林植物与人居环境。E-mail:2258855398@qq.com.责任作者：王雷（1972-），男，博士，教授，现主要从事草坪生态学与园林植物等研究工作。E-mail:14747405563@163.com.基金项目：内蒙古自治区应用技术研究与开发资金资助项目（2021GG0075）;乌海市全域矿山排土场植被生态恢复战略规划资助项目（WHZCS-G-F-230038）。收稿日期：2024-12-07