WANG Xiaomin,ZHANG Qinyu,CHEN Bo,et al.Effects of Five Heavy Metals Stress on Mycelia of Morchella sextelata[J].Northern Horticulture,2025,(10):78-85.[doi:10.11937/bfyy.20243892]
五种重金属胁迫对六妹羊肚菌菌丝体的影响
- Title:
- Effects of Five Heavy Metals Stress on Mycelia of Morchella sextelata
- 文章编号:
- 1001-0009(2025)10-0078-08
- Keywords:
- Morchella sextelata; heavy metal; accumulation; tolerance
- 分类号:
- S 646.7
- 文献标志码:
- A
- 摘要:
- 以六妹羊肚菌菌株MS630为试材，采用不同浓度的5种重金属离子培养基对羊肚菌菌株进行培养，研究了不同重金属处理对羊肚菌菌丝体生长发育过程中的菌丝生长速度、菌落形态、抑菌率、菌丝体生物量、菌丝重金属富集特性规律的影响，以期为后续羊肚菌耐重金属胁迫菌株选育提供参考依据。结果表明：同一重金属处理下，随着重金属浓度的增高，总体趋势是菌丝生长速度不断降低，抑菌率不断上升，菌丝密度下降，气生菌丝变弱，菌核密度下降。羊肚菌液体种在重金属胁迫下，随着重金属浓度的不断上升，菌丝生物量不断下降，抑菌率也不断增加，其中砷和汞对液体种菌丝影响较大，抑菌率最高。随着重金属浓度增高，羊肚菌菌丝重金属含量均不断增加，其中铅、镉和汞胁迫下，菌丝重金属富集程度较高，砷和铬处理下的重金属富集含量较低。因此，为确保羊肚菌绿色安全生产，人工种植MS630菌株的情况下，应该避免在铅、镉和汞污染严重的土地上栽培羊肚菌，以免其富集较多重金属，MS630在高浓度砷和铬胁迫下依然可以保持重金属低富集。
- Abstract:
- Taking Morchella sextelata strain MS630 as the test strain，five kinds of heavy metal ion culture media were used to culture Morchella strains.The effects of different heavy metal treatments on mycelial growth rate，colony morphology，antibacterial rate，mycelial biomass，and mycelial heavy metal enrichment characteristics during the growth and development of M.sextelata mycelium were studied,in order to provide reference for the subsequent breeding of M.sextelata strains resistant to heavy metal stress.The results showed that under the same heavy metal treatment，with the increase of the concentration of heavy metals，the overall trend was that the growth rate of mycelium decreased，the inhibition rate increased,the mycelium density decreased，the aerial hypha weakened，and the sclerotia density decreased.Under the stress of heavy metals，with the increasing concentration of heavy metals，the mycelial biomass of Morchella sextelata decreased，and the antibacterial rate also increased.Arsenic and mercury had a greater effect on the mycelia of liquid species，and the antibacterial rate was the highest.With the increase of the concentration of heavy metals，the content of heavy metals in M.sextelata mycelia increased continuously.Under the stress of lead，cadmium and mercury，the concentration of heavy metals in mycelia was higher，while the concentration of heavy metals in arsenic and chromium treatment was lower.Therefore，in order to ensure the green and safe production of Morchella，the cultivation of Morchella sextelata on the land with serious pollution of lead，cadmium and mercury should be avoided under the condition of artificial cultivation of MS630 strain，so as to prevent it from accumulating more heavy metals.MS630 could still maintain a low concentration of heavy metals under the stress of high concentrations of arsenic and chromium.
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备注/Memo
第一作者简介：王晓敏（1993-），男，硕士，助理研究员，现主要从事食用菌栽培育种等研究工作。E-mail：2512816249@qq.com.责任作者：张邦喜（1983-），男，博士，研究员，现主要从事食用菌栽培机理等研究工作。E-mail：zbx@cau.edu.cn.基金项目：国家重点研发计划资助项目（2022YFD1601704）；贵州省科技支撑计划资助项目（黔科合支撑［2022］一般083，黔科合支撑［2023］一般054）；国家食用菌产业技术体系资助项目（CARS-20）；贵州省食用菌产业技术体系资助项目（GZMARS-SYJ-2024-2026）；贵州省科研机构创新能力建设专项资金资助项目（黔科合服企［2021］1号）。收稿日期：2024-10-14