[1]阎君,梁荣,黄志午,等.不同温度对芹菜种子萌发的影响及促萌机理探究[J].北方园艺,2025,(4):31-37.[doi:10.11937/bfyy.20242978]
　YAN Jun,LIANG Rong,HUANG Zhiwu,et al.Effects of Different Temperature on Germination of Celery Seeds and Mechanism of Promoting Germination[J].Northern Horticulture,2025,(4):31-37.[doi:10.11937/bfyy.20242978]

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不同温度对芹菜种子萌发的影响及促萌机理探究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年4 页码: 31-37 栏目: 出版日期: 2025-02-28

Title:: Effects of Different Temperature on Germination of Celery Seeds and Mechanism of Promoting Germination

文章编号:: 1001-0009(2025)04-0031-07

作者:: 阎君1; 梁荣2; 黄志午1; 孟恒宇1; 何莉芳2; 朱为民1; （1.上海农业科学院设施园艺研究所，上海 201403；2.上海市松江区泖港镇农业农村服务中心，上海 201607）

Author(s):: YAN Jun1; LIANG Rong2; HUANG Zhiwu1; MENG Hengyu1; HE Lifang2; ZHU Weimin1; (1.Horticultural Research Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201403；2.Agricultural and Rural Service Center of Maogang Town，Songjiang District，Shanghai 201607)

关键词:: 芹菜种子; 赤霉素; 脱落酸; 休眠; 萌发

Keywords:: celery seeds; gibberellin; abscisic acid; dormancy; germination

分类号:: S 636.2

DOI:: 10.11937/bfyy.20242978

文献标志码:: A

摘要:: 以“上海黄心芹”为试材，设置5、10、15、20、25、30、35 ℃共7个温度处理，研究了不同温度处理下芹菜种子萌发过程中形态变化、生理变化和相关激素代谢基因的变化，以期为探究温度影响芹菜种子形态休眠的促萌机理提供参考依据。结果表明：20 ℃能显著促进芹菜种子萌发，萌发率达88%，发芽指数显著大于其他温度；5、10 ℃和35 ℃在相同时间内没有种子萌发。20 ℃能显著促进萌发期内芹菜种子胚的生长，萌发日胚∶种子比例达0.72。20 ℃提高了种子的抗氧化能力，并促进了萌发第1天赤霉素（GA）合成基因和脱落酸（ABA）降解基因的表达，推测可能在短时间内合成了较多的GA。25 ℃尽管多个指标与20 ℃相似，但最终萌发率仅有60%。研究还发现5 ℃缓慢促进种子胚生长，第10天胚∶种子为0.63，但35 ℃明显延缓了胚的生长，并且35 ℃可能通过抑制GA合成基因和ABA降解基因的表达使芹菜种子发生热休眠。说明20 ℃是芹菜种子发芽的最佳温度，低温延缓种子发芽，而高温会引发种子热休眠。

Abstract:: Taking ‘Shanghai Huang Yellow Heart’ as the experimental material，and 7 temperature treatments included 5，10，15，20，25，30 ℃ and 35 ℃ were set to study the morphological changes，physiological changes，and related hormone metabolism genes during celery seed germination under different temperature treatments，in order to provide reference for exploring explore the mechanism of temperature affecting celery seed morphological dormancy and promote germination.The results showed that 20 ℃ could significantly enhance the germination capacity of celery seeds，the germination rate reached 88%，and the germination index was significantly higher than other temperatures.No seeds germinated at 5 ℃,10 ℃，and 35 ℃ for the same time.Subsequent studies on the mechanism of germination at 20 ℃ showed that 20 ℃ could significantly promote the growth of celery seed embryos during germination，and the proportion of embryo,seed reached 0.72 on germination day.20 ℃ also increased the antioxidant capacity of seeds and promoted the expression of GA synthesis genes and ABA degradation genes on the first day of germination.It was speculated that more GA was synthesized in a short period of time.Although many parameters were similar to those of 20 ℃，the final germination rate was only 60% at 25 ℃.It was also found that 5 ℃ slowly promoted the growth of seed embryos，and the embryo,seed reached 0.63 on the tenth day，but 35 ℃ significantly delayed the growth of embryos，and 35 ℃ may cause thermal dormancy of celery seeds by inhibiting the expression of GA synthesis genes and ABA degradation genes.These results showed that 20 ℃ was the optimal temperature for celery seed germination.Low temperature delays seed germination，while high temperature could cause seed thermal dormancy.

参考文献/References:

［1］朱倩楠，张丽娟，曲继松．中部干旱带日光温室秋冬茬西芹栽培适应性比较［J］．黑龙江农业科学，2018(10)：95-97，107.［2］李涵，朱为民，阎君，等．不同红蓝光比例对芹菜幼苗生长和物质分配的影响［J］．北方园艺，2022(8)：41-45.［3］陈磊，陆信仁，王世忠，等．几种除草剂在直播芹菜田的应用技术研究［J］．长江蔬菜，2013(24)：62-64.［4］THRIAULT R K，PERREAULT M L．Hormonal regulation of circuit function：Sex，systems and depression［J］．Biology of Sex Differences，2019，10(1)：12.［5］ALI S，KHAN N，XIE L．Molecular and hormonal regulation of leaf morphogenesis in Arabidopsis［J］．International Journal of Molecular Sciences，2020，21(14)：5132.［6］WALKER M，PREZ M，STEINBRECHER T，et al．Molecular mechanisms and hormonal regulation underpinning morphological dormancy：A case study using Apium graveolens (Apiaceae)［J］．The Plant Journal，2021，108(4)：1020-1036.［7］JACOBSEN J V，PRESSMAN E．A structural study of germination in celery (Apium graveolens L．) seed with emphasis on endosperm breakdown［J］．Planta，1979，144(3)：241-248.［8］NONOGAKI H．Seed germination and dormancy：The classic story，new puzzles，and evolution［J］．Journal of Integrative Plant Biology，2019，61(5)：541-563.［9］王晓龙，杨曌，李红，等.6个苜蓿品种种子萌发对低温的响应［J］．中国草地学报，2022，44(7)：79-86.［10］ARC E，GALLAND M，GODIN B，et al．Nitric oxide implication in the control of seed dormancy and germination［J］．Frontiers in Plant Science，2013(4)：346.［11］KLUPCZYN〖JX-+0.8mm〗〖KG-*2〗＇SKA E A，PAWOWSKI T A．Regulation of seed dormancy and germination mechanisms in a changing environment［J］．International Journal of Molecular Sciences，2021，22(3)：1357.［12］YANG L，LIU S，LIN R．The role of light in regulating seed dormancy and germination［J］．Journal of Integrative Plant Biology，2020，62(9)：1310-1326.［13］邵力鑫．温度对芹菜种子发芽的影响及其生理效应［J］．河南科技，1991，10(6)：14-15.［14］古吉，马文广．不同萌发温度下红花大金元种子引发温度的筛选研究［J］．种子，2017，36(3)：16-20.［15］YAMAUCHI Y，OGAWA M，KUWAHARA A，et al．Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds［J］．The Plant Cell，2004，16(2)：367-378.［16］郭聪聪，沈永宝，史锋厚．白皮松种子萌发过程中呼吸代谢和内源激素对温度变化的响应［J］．中南林业科技大学学报，2021，41(3)：25-36.［17］高婷婷，毛培春，郭强，等．温度对10个白三叶品种种子萌发特性的影响［J］．山西农业大学学报(自然科学版)，2014，34(1)：59-64.［18］蔡子文，王宏霞，蔡子平，等.引发处理对不同温度下送蓝种子萌发与幼苗生长的生态效应［J］.北方园艺，2023(20)：102-109.［19］WANG H，ZHANG Y，XIAO N，et al．Rice GERMIN-LIKE PROTEIN 2-1 functions in seed dormancy under the control of abscisic acid and gibberellic acid signaling pathways［J］．Plant Physiology，2020，183(3)：1157-1170.［20］FINCH-SAVAGE W E，LEUBNER-METZGER G．Seed dormancy and the control of germination［J］．New Phytologist，2006，171(3)：501-523.［21］卢艳，闫月，崔程程，等．初生休眠解除状态和干燥处理对水曲柳种子萌发的影响［J］．植物研究，2020，40(4)：490-495.［22］宋顺华，宋松泉，吴萍，等．白菜种子萌发的热抑制现象及其与细胞壁降解酶的关系［J］．园艺学报，2014，41(6)：1115-1124.［23］张勇，薛林贵，高天鹏，等．荒漠植物种子萌发研究进展［J］．中国沙漠，2005，25(1)：106-112.［24］HE H，DE SOUZA VIDIGAL D，SNOEK L B，et al．Interaction between parental environment and genotype affects plant and seed performance in Arabidopsis［J］．Journal of Experimental Botany，2014，65(22)：6603-6615.［25］URANO K，MARUYAMA K，JIKUMARU Y，et al．Analysis of plant hormone profiles in response to moderate dehydration stress［J］．The Plant Journal，2017，90(1)：17-36.

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备注/Memo

第一作者简介：阎君(1983-)，女，博士，副研究员，现主要从事芹菜栽培与遗传育种等研究工作。E-mail：ylgw_07@126.com.责任作者：朱为民(1969-)，男，博士，研究员，现主要从事蔬菜栽培与遗传育种等研究工作。E-mail：wmzhu69@126.com.基金项目：上海市科委农业领域资助项目(23N71900200)；上海市农业科技创新项目技术攻关资助项目（2023-02-08-00-12-F04620）。收稿日期：2022-08-05

更新日期/Last Update: 2025-02-28