QIAN Lijuan,XU Chengxuan,JIANG Shengjuan,et al.Study on the Enrichment of Selenium in Hericium erinaceus Mycelium[J].Northern Horticulture,2024,(16):109-115.[doi:10.11937/bfyy.20240473]
猴头菇菌丝体富集硒的研究
- Title:
- Study on the Enrichment of Selenium in Hericium erinaceus Mycelium
- 文章编号:
- 1001-0009(2024)16-0109-07
- Keywords:
- Hericium erinaceus; selenium-enriched fermentation; active substances; antioxidant activity
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以4种猴头菇为出发菌株，采用平板和液体发酵培养方式，研究了亚硒酸钠和酵母硒对4种猴头菇菌丝生长的影响，比较了不同硒浓度培养的菌丝体生长速率、硒含量及富硒菌丝体水提物的蛋白质、多糖、总酚含量及抗氧化活性，以期为富硒猴头菇功能食品的开发及应用提供参考依据。结果表明：“猴头911”“猴头920”“猴头王”“猴头3008” 4种菌株中，“猴头911”菌株富硒能力较强，且在酵母硒培养中硒含量最高。硒能够提高猴头菇菌丝体蛋白质、多糖、总酚等主要活性物质含量。当“猴头911”富硒菌丝体水提物质量浓度为2 mg·mL-1时，羟基自由基、超氧阴离子自由基和1,1-二苯基-2-苦味基肼(DPPH)自由基的最高清除率分别为67.53%、39.27%、74.05%，均高于对照。
- Abstract:
- Four Hericium erinaceus was used as the starting strain,and plate and liquid fermentation culture methods were used.The effects of sodium selenite and selenium yeast on the growth of four species of Hericium erinaceus mycelium,compared the growth rate,selenium content,protein level,polysaccharide concentration,total phenol content,and antioxidant activity of the aqueous extract enriched with selenium-enriched mycelium with different concentrations of selenium,in order to provide reference for the development and application of selenium enrichment functional food of Hericium erinaceus.The results showed that among the four strains of ‘Hericium erinaceus 911’‘Hericium erinaceus 920’‘Hericium erinaceus king’‘Hericium erinaceus3008’‘Hericium erinaceus 911’ had stronger selenium-enriched ability and the highest selenium content in yeast selenium culture.Selenium could increase the content of protein,polysaccharide,total phenol and other main active substances in mycelium of Hericium erinaceus.The highest scavenging rates of hydroxyl free radical,superoxide anion free radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical were 67.53%,39.27% and 74.05%,respectively,when the concentration of the water extract of selenium-rich mycelium of ‘Hericium erinaceus 911’ was 2 mg·mL-1,which were higher than those of control.
参考文献/References:
［1］杜颜宇,雷雨霞,潘尚丽,等.甘蔗渣不同配方栽培猴头菇试验［J］.北方园艺,2015(23):150-152.［2］杨杰,赵淇,张乐,等.猴头菇多糖保健功效的研究进展［J］.农产品加工,2021(12):79-80,85.［3］李浩男,谢晓宇,王立平.硒营养与人体健康［J］.食品工业,2022,43(3):325-330.［4］ZHOU Q,ZHANG B,CHEN X,et al.Association of serum selenium with anemia-related indicators and risk of anemia［J］.Food Science & Nutrition,2021,9(6):3039-3047.［5］ZHANG D,ZHANG D,YANG X,et al.The role of selenium-mediated notch/Hes1 signaling pathway in kashin-beck disease patients and cartilage injury models［J］.Biological Trace Element Research,2023,201(6):2765-2774.［6］宋丽,王跃,付丹,等.硒在糖尿病中抗氧化的作用及研究进展［J］.生理科学进展,2023,54(1):76-80.［7］荀黎红,吴丛雅.硒与癌症关系的研究现状［J］.国外医学(医学地理分册),2006,27(3):100-102.［8］兰凉英.酵母硒对秀珍菇产量与主要营养成分含量的影响［J］.食用菌,2020,42(5):34-36.［9］牛建峰,王广策,曾呈奎,等.带形蜈蚣藻多糖和可溶性蛋白含量测定及藻红蛋白分析鉴定［J］.海洋科学,2006,30(8):50-53.［10］李红英,陈菲菲,孙举志,等.硒对香菇品质的影响［J］.北方园艺,2022(12):106-113.［11］李春兰,李佳欢,胡开辉,等.高抗氧化活性黑皮鸡枞优良菌株筛选［J］.南方农业,2023,17(15):29-33.［12］卢彩会,郭晓萌,牟德华.黑皮鸡枞菌水提物和醇提物的主要成分及抗氧化性研究［J］.食品工程,2016(3):35-40,57.［13］王华,刘虹,刘欣,等.富硒黄白侧耳子实体蛋白质和多糖的研究［J］.中国食用菌,2018,37(3):59-63.［14］DONG Z,XIAO Y,WU H.Selenium accumulation,speciation,and its effect on nutritive value of Flammulina velutipes (Golden needle mushroom)［J］.Food Chemistry,2021,350:128667.［15］徐联鹏,吴萍,陆晨晖,等.富硒秀珍菇的培育及其营养品质评价［J］.南京晓庄学院学报,2021,37(6):53-58.［16］金鑫,熊川,黄文丽,等.灵芝菌丝体富硒条件优化及其硒多糖抗氧化活性研究［J］.天然产物研究与开发,2020,32(1):1-10.［17］HELENO S A,BARROS L,MARTINS A,et al.Chemical composition,antioxidant activity and bioaccessibility studies in phenolic extracts of two Hericium wild edible species［J］.LWT-Food Science and Technology,2015,63(1):475-481.［18］MALINOWSKA E,KRZYCZKOWSKI W,HEROLD F,et al.Biosynthesis of selenium-containing polysaccharides with antioxidant activity in liquid culture of Hericium erinaceum［J］.Enzyme and Microbial Technology,2009,44(5):334-343.［19］冮洁,麦海美,解彬,等.羊肚菌菌丝体富硒条件优化及其硒多糖抗氧化活性研究［J］.食品与发酵工业,2016,42(9):120-125.［20］冮洁,张文静,季旭颖.美味牛肝菌富硒培养条件优化及其多糖抗氧化性研究［J］.中国食品学报,2015,15(12):91-98.［21］陆晓民,桂艳华,陈义伟.微量元素硒在猴头菇栽培中的应用研究初探［J］.中国林副特产,2006(2):23-24.［22］李好.富硒猴头栽培及其药理活性初探［D］.合肥:安徽农业大学,2010.［23］张彩妮,邓百万,柏秋月,等.富硒条件下蜜环菌菌株硒耐受性及胞外酶生物活性的研究［J］.微生物学报,2022,62(2):640-649.［24］陈雪凤,刘增亮,吴圣进,等.秀珍菇富硒菌株筛选及其子实体富硒规律研究［J］.中国食用菌,2023,42(4):34-40.
相似文献/References:
[1]李凤美,周永斌,张志军,等.不同出菇方式对袋栽猴头菇的影响[J].北方园艺,2013,37(22):149.
LI Feng-mei,ZHOU Yong-bin,ZHANG Zhi-jun,et al.Effect of Different Fruiting Ways on Bag Cultured Hericium erinaceus Pers.[J].Northern Horticulture,2013,37(16):149.
[2]吴清山.猴头菇高效栽培关键技术[J].北方园艺,2014,38(18):161.
WU Qing-shan.Key Technologies of Efficient Cultivation of Hericium erinaceus[J].Northern Horticulture,2014,38(16):161.
[3]邓政东,程爱芳,郑廷金.猴头菇凝集素的提取工艺研究[J].北方园艺,2014,38(24):133.
DENG Zheng-dong,CHENG Ai-fang,ZHENG Ting-jin.Study on Extraction Technology of Lectin from Hericium erinaceus[J].Northern Horticulture,2014,38(16):133.
[4]倪淑君,王延锋,张海峰,等.寒地猴头菇菌种筛选试验[J].北方园艺,2015,39(02):127.[doi:10.11937/bfyy.201502036]
NI Shu-jun,WANG Yan-feng,ZHANG Hai-feng,et al.Screening Test of Hericium rinaceus Strains in Cold Regions[J].Northern Horticulture,2015,39(16):127.[doi:10.11937/bfyy.201502036]
[5]倪淑君,张海峰,王延锋,等.不同配方袋栽猴头菇试验[J].北方园艺,2015,39(11):138.[doi:10.11937/bfyy.201511034]
NI Shu-jun,ZHANG Hai-feng,WANG Yan-feng,et al.Study on Different Formula Bag Cultivation on the Influence of Hericium[J].Northern Horticulture,2015,39(16):138.[doi:10.11937/bfyy.201511034]
[6]潘 漫,盛 园 园,汤 玉 亮,等.棉籽壳菠萝皮混合培养基栽培猴头菇试验初探[J].北方园艺,2010,34(17):0.[doi:10.11937/bfyy.201017083]
,,et al.Ｎ ｅ ｗ ｌ ｙ Ｔ ｅ ｓ ｔ ｏ ｎ Ｃ ｕ ｌ ｔ ｉ ｖ ａ ｔ ｉ ｎ ｇ Ｈ ｅ ｒ ｉ ｃ ｉ ｕ ｍ ｅ ｒ ｉ ｎ ａ ｃ ｅ ｕ ｓ ｗ ｉ ｔ ｈ ｔ ｈ ｅ Ｍ ｉ ｘ ｅ ｄＭ ｅ ｄ ｉ ｕ ｍ ｏ ｆ Ｃ ｏ ｔ ｔ ｏ ｎ Ｓ ｅ ｅ ｄ Ｈ ｕ ｌ ｌ ｓ ａ ｎ ｄ Ｐ ｉ ｎ ｅ ａ ｐ ｐ ｌ ｅ Ｐ ｅ ｅ ｌ[J].Northern Horticulture,2010,34(16):0.[doi:10.11937/bfyy.201017083]
[7]杜颜宇,雷雨霞,潘尚丽,等.甘蔗渣不同配方栽培猴头菇试验[J].北方园艺,2015,39(23):150.[doi:10.11937/bfyy.201523042]
DU Yanyu,LEI Yuxia,PAN Shangli,et al.Study on Different Bagasse Formulas on Cultivation of Hericium erinacius[J].Northern Horticulture,2015,39(16):150.[doi:10.11937/bfyy.201523042]
[8]王 玉,李 政,班 立 桐,等.猴头菇液体菌种培养基配方的研究[J].北方园艺,2011,35(09):0.[doi:10.11937/bfyy.201109074]
W ANG Yu,LI Zheng,BAN Li -tong,et al.Optimization of Fermentation Medium for Improving Mycelium of Hericiumerinaceus ( Bull. ) Per .[J].Northern Horticulture,2011,35(16):0.[doi:10.11937/bfyy.201109074]
[9]郝 涤 非.猴 头 菇 含 氮 物 质 测 定[J].北方园艺,2011,35(23):0.[doi:10.11937/bfyy.2011023055]
Ｄ ｅ ｔ ｅ ｒ ｍ ｉ ｎ ａ ｔ ｉ ｏ ｎ ｏ ｆ Ｎ ｉ ｔ ｒ ｏ ｇ ｅ ｎ － ｃ ｏ ｎ ｔ ａ ｉ ｎ ｉ ｎ ｇ Ｓ ｕ ｂ ｓ ｔ ａ ｎ ｃ ｅ ｓ ｏ ｆ Ｈ ｅ ｒ ｉ ｃ ｉ ｕ ｍ[J].Northern Horticulture,2011,35(16):0.[doi:10.11937/bfyy.2011023055]
[10]魏雅冬,李艳芳,王广慧,等.家庭环境培养灵芝、猴头菇、榆黄蘑盆景的初步研究[J].北方园艺,2018,42(02):177.[doi:10.11937/bfyy.20172077]
WEI Yadong,LI Yanfang,WANG Guanghui,et al.Preliminary Study on Bonsai Cultivation of Ganoderma lucidum, Hericium erinaceus and Pleurotus citrinopileatus in Home Environment[J].Northern Horticulture,2018,42(16):177.[doi:10.11937/bfyy.20172077]
备注/Memo
第一作者简介：钱丽娟（1998-），女，硕士研究生，研究方向为猴头菇富硒栽培及主要活性物质的提取。E-mail：qlj2560@163.com.责任作者：孙玉军（1975-），男，博士，教授，现主要从事富硒食用菌栽培及活性成分分析等研究工作。E-mail：sunyujun208@163.com.基金项目：安徽省高校协同创新资助项目（GXXT-2023-054）；安徽省高校自然科学研究重大资助项目（KJ2021ZD0107）；安徽科技学院科研发展基金资助项目（FZ220105）。收稿日期：2024-01-29