CHEN Jia,YANG Cairong,QI Weiliang,et al.Effects of PGPR Bacteria on Physiological Characteristics of Ryegrass Plants Under Salt Stress[J].Northern Horticulture,2024,(10):55-60.[doi:10.11937/bfyy.20233451]
盐胁迫下PGPR菌剂对黑麦草植株生理特性的影响
- Title:
- Effects of PGPR Bacteria on Physiological Characteristics of Ryegrass Plants Under Salt Stress
- 文章编号:
- 1001-0009(2024)10-0055-06
- Keywords:
- salt stress; physiological index; ryegrass
- 分类号:
- S 543+.6
- 文献标志码:
- A
- 摘要:
- 以黑麦草为试材，采用盆栽试验法，研究了不同浓度NaCl处理下接种植物根际促生菌芽孢杆菌CP5(T1)、LM2(T2)、T3（混合菌剂，T1∶T2=1∶1）对黑麦草生理特性的影响，以期为PGPR菌剂的应用提供参考依据。结果表明：1）接种T1菌剂能在较高浓度（500、700 mmol·L-1）盐胁迫下降低丙二醛（MDA）含量、提高可溶性糖含量及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性；2）接种T2菌剂后在较高盐浓度（500、700 mmol·L-1）胁迫下降低黑麦草MDA含量，提高可溶性糖含量及过氧化物酶（POD）、SOD、CAT活性；3）不同浓度盐分胁迫下，接种T3菌剂均可提高可溶性糖含量、脯氨酸（Pro）含量、POD、SOD、CAT活性，降低MDA含量。尤其是在300 mmol·L-1盐胁迫下，接种T3植株较未接种菌剂组SOD活性显著上升44.42%，500 mmol·L-1盐胁迫下，接种T3植株较未接种菌剂组Pro含量、可溶性糖含量、CAT活性分别显著上升17.42%、100.74%、91.60%，700 mmol·L-1盐胁迫下，接种T3菌剂植株较未接种菌剂组MDA含量显著下降43.83%、POD活性显著上升69.29%（P<0.05）。综上所述，接种根际促生菌芽孢杆菌有助于缓解植物生长时受到的盐胁迫，且混合菌剂缓解盐胁迫的效果显著大于单个菌剂，具有开发应用为盐胁迫环境植物生长菌剂潜能。
- Abstract:
- Taking ryegrass as the test material,a pot experiment was conducted to study the effects of inoculating rhizosphere growth promoting bacteria Bacillus subtilis CP5 (T1),LM2 (T2),T3 (mixed bacterial agent,T1∶T2=1∶1) on the physiological characteristics of ryegrass under different concentrations of NaCl treatments,in order to provide reference for PGPR application.The results showed that,1) inoculation with T1 bacterial agent could reduce MDA content,increase soluble sugar content,CAT activity,and SOD activity under relatively high concentration (500,700 mmol·L-1 NaCl) salt stress.2) After inoculation with T2 bacterial agent,the MDA content of ryegrass was reduced and the soluble sugar content,POD,SOD,and CAT activities were increased under relatively high concentration (500,700 mmol·L-1 NaCl) salt stress.3) Under different concentrations of salt stress,inoculation with T3 bacterial agent could increase soluble sugar content,proline (Pro) content,peroxidase (POD),superoxide dismutase (SOD),catalase (CAT) activity,and reduce malondialdehyde (MDA) content.Especially under 300 mmol·L-1 salt stress,the SOD activity of T3 inoculated plants significantly increased by 44.42% compared to the non inoculated group.Under 500 mmol·L-1 salt stress,the Pro content,soluble sugar content,and CAT activity of T3 inoculated plants significantly increased by 17.42%,100.74%,and 91.60% compared to the non inoculated group,respectively.Under 700 mmol·L-1 salt stress,the MDA content of T3 inoculated plants significantly decreased by 43.83% and POD activity significantly increased by 69.29% compared to the non inoculated group (P<0.05).In conclusion,this study indicated that inoculation with rhizosphere growth promoting bacteria Bacillus subtilis could help alleviate salt stress on plant growth,and the effect of mixed microbial agents on alleviating salt stress was significantly greater than that of single microbial agents,indicating the potential for development and application as a growth microbial agent for plants under salt stress.
参考文献/References:
［1］温宏伟,杨斌,王东胜.植物根际促生菌促进小麦生长及提高其抗旱性的研究进展［J］.核农学报,2021,35(9):2194-2203.［2］李琬,刘淼,张必弦,等.植物根际促生菌的研究进展及其应用现状［J］.中国农学通报,2014,30(24):1-5.［3］胡亚杰,韦建玉,卢健,等.枯草芽孢杆菌在农作物生产上的应用研究进展［J］.作物研究,2019,33(2):167-172.［4］孙鹏波,王志军,格根图，等.紫花苜蓿耐盐碱胁迫与缓减措施的研究进展［J］.北方园艺,2023(21):131-137.［5］TIRRY N,KOUCHOU A,LAGHMARI G,et al.Improved salinity tolerance of Medicago sativa and soil enzyme activities by PGPR［J］.Biocatalysis and Agricultural Biotechnology,2021（31）:101914.［6］王华笑.解淀粉芽孢杆菌YM6对盐胁迫下玉米促生作用及机理研究［D］.银川:北方民族大学,2020.［7］胡玉婕,朱秀玲,丁延芹,等.芽孢杆菌的耐盐促生机制研究进展［J］.生物技术通报,2020,36(9):64-74.［8］穆文强,康慎敏,李平兰.根际促生菌对植物的生长促进作用及机制研究进展［J］.生命科学,2022,34(2):118-127.［9］陈可,胡南,陈威,等.两种植物根际促生菌对博落回抗干旱及富集铀性能的增强作用研究［J］.环境科学学报,2018,38(10):4142-4149.［10］MANJUNATHA B S,NIVETHA N,KRISHNA G K,et al.Plant growth-promoting rhizobacteria Shewanellaputrefaciens and Cronobacter dublinensis enhance drought tolerance of pearl millet by modulating hormones and stress-responsive genes［J］.Physiologia Plantarum,2022,174(2):e13676.［11］陈苏,谢建坤,黄文新,等.根际促生细菌对干旱胁迫下水稻生理特性的影响［J］.中国水稻科学,2018,32(5):485-492.［12］苏迅帆.枯草芽孢杆菌对拟南芥的干旱和盐胁迫耐受性的影响［J］.分子植物育种,2022,20(2):536-543.［13］CHAHAL G K,KAUR A,GHAI N.Mitigation of salt stress with Azospirillium and Azotobacter inoculation in maize (Zea maysL.)［J］.Cereal Research Communications,2022,50(4):915-927.［14］张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法［J］.植物生理学通讯,1990,26(4):62-65.［15］丁雪梅,张晓君,赵云,等.蒽酮比色法测定可溶性糖含量的试验方法改进［J］.黑龙江畜牧兽医,2014(23):230-233.［16］张清航,张永涛.植物体内丙二醛(MDA)含量对干旱的响应［J］.林业勘查设计,2019(1):110-112.［17］ 冯晓硕.根际促生菌筛选鉴定及其对连作大豆的促生作用［D］.大庆:黑龙江八一农垦大学,2021.［18］梁杰,杨鹭生.黄连木叶过氧化物酶活性及其同工酶酶谱分析［J］.广州化工,2012,40(14):57-59,82.［19］吴志明,钟敏,鹿承建,等.新型农抗N2粗提物对水稻纹枯病菌细胞膜和抗氧化功能的影响［J］.核农学报,2018,32(4):700-707.［20］THAPA G,DEY M,SAHOO L,et al.An insight into the drought stress induced alterations in plants［J］.Biologia Plantarum,2011,55(4):603.［21］JIANG M,ZHANG J.Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves［J］.Journal of Experimental Botany,2002,53(379):2401-2410.［22］NOREEN Z,ASHRAF M,AKRAM N A.Salt-induced regulation of some key antioxidant enzymes and physio-biochemical phenomena in five diverse cultivars of turnip (Brassica rapaL.)［J］.Journal of Agronomy and Crop Science,2010,196(4):273-285.［23］AYUSO-CALLES M,GARCA-ESTVEZ I,JIMNEZ-GMEZ A,et al.Rhizobium laguerreae improves productivity and phenolic compound content of lettuce (Lactuca sativa L.) under saline stress conditions［J］.Foods,2020,9(9):1166.［24］兰汝佳.根际耐盐促生菌的筛选及其对番茄耐盐性的调控研究［D］.南京:南京农业大学,2019.［25］LEE D G,LEE J M,CHOI C G,et al.Effect of plant growth-promoting rhizobacterial treatment on growth and physiological characteristics of Triticum aestivum L.under salt stress［J］.Applied Biological Chemistry,2021,64(1):89.［26］HAHM M S,SON J S,HWANG Y J,et al.Alleviation of salt stress in pepper (Capsicum annum L.) plants by plant growth-promoting rhizobacteria［J］.Journal of Microbiology and Biotechnology,2017,27(10):1790.［27］辛树权,郭菊卉,赵骥民.NaCl胁迫下促生菌对黄瓜幼苗生长的促生效应［J］.北方园艺,2009(12):46-49.［28］邓勋,宋小双,尹大川,等.盐胁迫下深色有隔内生真菌D575对樟子松生长及耐盐性的影响［J］.森林工程,2015,31(6):5-10,17.［29］程田.堆肥微生物菌剂对草坪植物及土壤生态的影响［D］.天津:天津师范大学,2012.［30］陈英华,严重玲,李裕红,等.盐胁迫下红海榄脯氨酸与活性氧代谢特征研究［J］.厦门大学学报(自然科学版),2004,43(3):402-405.
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备注/Memo
第一作者简介：陈佳（2001-），女，硕士研究生，研究方向为植物重金属胁迫生理。E-mail：1615425285@qq.com.责任作者：杨财容(1988-)，女，博士，副教授，现主要从事植物资源研究与利用、植物系统演化等研究工作。E-mail:762778683@qq.com.基金项目：四川省大学生创新创业训练计划资助项目（S202214389141）；成都师范学院高级别科研项目培育专项资助项目（CS22XMPY0106）。收稿日期：2023-10-11