YANG Fenfen,GuliziyeHALIKE,AilijiangMAIMAITI,et al.Effects of Exogenous Trehalose on Photosynthesis of Hami Melon Seedlings Under Chilling Stress[J].Northern Horticulture,2023,(05):27-38.[doi:10.11937/bfyy.20222972]
外源海藻糖对低温胁迫下哈密瓜幼苗光合作用的影响
- Title:
- Effects of Exogenous Trehalose on Photosynthesis of Hami Melon Seedlings Under Chilling Stress
- 文献标志码:
- A
- 摘要:
- 以“西州密25号”幼苗为试材，采用0、5、10、20 mmol?L-1外源海藻糖预处理3 d，在昼/夜温度分别为15 ℃/10 ℃、10 ℃/5 ℃、5 ℃/1 ℃低温胁迫1 d和3 d时测定幼苗叶片的光合色素含量、净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）和胞间CO2浓度（Ci），研究了在低温条件下外源海藻糖对哈密瓜 (Cucumis melon var.saccharins) 幼苗光合作用的影响，明确其耐寒调控机理，以期为缓解倒春寒气候对哈密瓜幼苗造成的伤害提供参考依据。结果表明：在未喷施外源海藻糖情况下，低温胁迫显著抑制了叶片的光合能力，且叶绿素b（Chl b）含量显著增加，随着温度的降低，其它光合色素含量与Pn、Gs、Ci和Tr均显著下降；与未喷施外源海藻糖相比，外源海藻糖可对低温胁迫下哈密瓜幼苗的一系列光合指标产生影响，适宜浓度的海藻糖可明显提高光合色素含量，增强哈密瓜叶片Pn、Gs和Tr。通过隶属函数综合评价可知，低温胁迫下5、10 mmol?L-1外源海藻糖明显促进了叶绿素a（Chl a）、Chl b、类胡萝卜素（Car）的积累，并显著提高了光合作用能力，对幼苗光合特性的综合缓解效果最好，而外源海藻糖浓度过高，其缓解幼苗低温胁迫的效应降低。
- Abstract:
- The seedlings of ‘Xizhoumi No.25’ were used as experimental material,0,5,10,20 mmol?L-1 exogenous trehalose were pretreated for 3 days.The photosynthetic pigment content,net photosynthetic rate (Pn),transpiration rate (Tr),stomatal conductance (Gs) and intercellular CO2 concentration (Ci) of seedling leaves were measured under low temperature stress of 15 ℃/10 ℃，10 ℃/5 ℃，5 ℃/1 ℃ for 1 day and 3 days.The effects of exogenous trehalose on photosynthesis of hami melon (Cucumis melon var.saccharins) seedlings under low temperature conditions were studied,and the mechanism of cold tolerance regulation was clarified,in order to provide reference for alleviating the damage of hami melon seedlings caused by late spring cold climate.The results showed that chilling stress markedly inhibited the photosynthesis ability of leaves compared to those of non-trehalose sprayed plants,moreover,the content of chlorophyll b (Chl b) increase significantly,the content of other photosynthetic pigments and Pn,Gs,Ci and Tr were decreased significantly as the temperature decreases;however,exogenously applied trehalose markedly alleviated the chilling-induced drastic effects on gas exchange attributes of hami melon seedlings under chilling stress.Appropriate concentration of trehalose could significantly increase the content of photosynthetic pigment and enhance the net photosynthetic rate,stomatal conductance and transpiration rate of melon leaves compared to non-trehalose-treated seedlings.Of the trehalose concentrations,the comprehensive evaluation of membership function method results indicate that the highest chilling tolerance was obtained with 5 mmol?L-1 and 10 mmol?L-1 trehalose pre-treatment.These results suggested that increased chilling tolerance might be occurred through trehalose significantly promoted the accumulation of chlorophyll a (Chl a),Chl b and carotenoids,and improved the photosynthetic capacity under chilling stress.However,the high concentration of exogenous trehalose will reduce the mitigation effect of chilling stress.
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备注/Memo
第一作者简介：杨芬芬（1998-），女，硕士研究生，研究方向为园艺作物栽培与生理。E-mail:1983299913@qq.com.责任作者：艾力江?麦麦提（1981-），男，博士，讲师，现主要从事植物逆境生理生态等研究工作。E-mail:ailijanmmt@126.com.基金项目：新疆维吾尔自治区自然科学基金计划-特培资助项目（2019D03016）；新疆维吾尔自治区人力资源与社会保障厅高层次人才引进工程资助项目（2017-2020）；新疆农业大学大学生创新训练计划资助项目（dxscx2020329）。收稿日期：2022-07-19