[1]王宏霞,李文麒.蒙古韭叶绿体微卫星特征分析[J].北方园艺,2023,(03):1-7.[doi:10.11937/bfyy.20222346]
　WANG Hongxia,LI Wenqi.Characterization Analysis of Microsatellite in Chloroplast of Allium mongolicum[J].Northern Horticulture,2023,(03):1-7.[doi:10.11937/bfyy.20222346]
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蒙古韭叶绿体微卫星特征分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年03 页码: 1-7 栏目: 出版日期: 2023-02-15
Title:
Characterization Analysis of Microsatellite in Chloroplast of Allium mongolicum
作者:
王宏霞1; 2; 3; 李文麒1
（1.青海民族大学 生态环境与资源学院，青海 西宁 810000；2.青海省特色经济植物高值化利用重点实验室，青海 西宁 810000；3.生物技术与分析测试实验室，青海 西宁 810000）
Author(s):
WANG Hongxia1; 2; 3; LI Wenqi1
(1.College of Ecological Environment and Resources,Qinghai University for Nationalities,Xining,Qinghai 810000;2.Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants,Xining,Qinghai 810000;3.Laboratory of Biotechnology and Analytical Testing,Xining,Qinghai 810000)
关键词:
蒙古韭; 叶绿体; 微卫星
Keywords:
Allium mongolicum; chloroplast; microsatellite
DOI:
10.11937/bfyy.20222346
文献标志码:
A
摘要:
以蒙古韭为试材，采用Illumina NovaSep 高通量测序技术对叶绿体基因组进行测序，MISA v1.0 软件对叶绿体基因组微卫星进行搜索，研究了蒙古韭叶绿体微卫星特征，以期为蒙古韭的鉴定、居群遗传学研究和系统发育研究提供参考依据。结果表明：在蒙古韭153 376 bp的叶绿体全基因组序列上共识别出260个SSR位点，平均每590 bp出现一个，主要分布于大拷贝区。cpSSR重复序列共有5种类型，其中单碱基重复序列最多，为181个，约占重复序列总量的66.79%，其次是三碱基重复序列，共65个，约占重复序列总量的23.99%，五碱基最少，仅占0.37%。cpSSR长度区间为8~29 bp，从整体来看，主要集中在8~10 bp，在该范围内共有197个，占SSR总数的75.77%；长度≥20 bp的有9个，结果表明蒙古韭叶绿体基因组上的微卫星位点大多数具有多态性的潜能。
Abstract:
Taking Allium mongolicum as test material,the chloroplast genome was sequenced by Illumina NovaSep high-throughput sequencing technology,and the chloroplast genome microsatellites were searched and analyzed by using MISA v1.0 software,in order to provide reference for the species identification,population genetics and phylogenetic studies of A.mongolicum.The results showed that a total of 260 SSR sites were detected on the 153 376 bp chloroplast genome sequence of A.mongolicum.One SSR locus occurred per 590 bp on average,mainly distributed in the large copy region.There were 5 repeat types,of which the largest number of single nucleotide repeat type accounted for 66.79%,followed by trinucleotide (23.99%) and the least number of pentanucleotide,accounting for only 0.37%.The cpSSR was 8-29 bp,mainly in the range of 8-10 bp (75.77%),of which 9 cpSSR were 20 bp and above in length,indicating that the cpSSR on the chloroplast genome of A.mongolicum have a high polymorphic potential.

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备注/Memo

第一作者简介：王宏霞(1986-)，女，博士，副教授，现主要从事蔬菜分子植物育种等研究工作。E-mail:zj511102365@163.com.基金项目：青海省自然科学基金资助项目(2019-ZJ-984Q)。收稿日期：2022-06-07

更新日期/Last Update: 2023-02-21